Team:Tsinghua/Result3
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[[Image:j43.png|300px|center|j43|thumb|pET-28a+Targeted Biobrick]] | [[Image:j43.png|300px|center|j43|thumb|pET-28a+Targeted Biobrick]] | ||
- | [[Image:j44.png|600px|center|j44|thumb|Identification of the Targeted Biobrick positive clones. Desired bands are at | + | [[Image:j44.png|600px|center|j44|thumb|Identification of the Targeted Biobrick positive clones. Desired bands are at 1260 bp]] |
The expression vector was used for Targeted Biobrick characterization, while the standardized Targeted Biobrick has been submitted to iGEM headquarters for parts registry. We have successfully gained the desired Targeted Biobrick protein of 44184.58 Daltons. | The expression vector was used for Targeted Biobrick characterization, while the standardized Targeted Biobrick has been submitted to iGEM headquarters for parts registry. We have successfully gained the desired Targeted Biobrick protein of 44184.58 Daltons. |
Revision as of 14:40, 19 October 2009
Targeted Biobrick Construction
In this section, we were contructing two contructs in paralell. First, standardized biobrick for parts registry, where Targeted Biobrick can be served for further use by other teams or researchers. Second, incorporate Targeted Biobrick into an expression vector pET-28a to test its expression. Targeted Biobrick was contructed via two rounds of PCR.
The expression vector was used for Targeted Biobrick characterization, while the standardized Targeted Biobrick has been submitted to iGEM headquarters for parts registry. We have successfully gained the desired Targeted Biobrick protein of 44184.58 Daltons.