Team:Tsinghua/Result3
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GuoQiangChen (Talk | contribs) (→Targeted Biobrick Construction) |
GuoQiangChen (Talk | contribs) (→Targeted Biobrick Characterization by Fusing with GFP) |
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=Targeted Biobrick Characterization by Fusing with GFP= | =Targeted Biobrick Characterization by Fusing with GFP= | ||
- | [[Image:transfection.png|800px|center|transfection]] | + | [[Image:transfection.png|800px|center|thumb|Tranfection of pEGFP+Targeted Biobrick into HeLa Cells. A.tranfected HeLa cells by pEGFP+Targeted Biobrick at bright field; B.tranfected HeLa cells by pEGFP+Targeted Biobrick under fluorescent microscopy at the same field; C.tranfected HeLa cells by pEGFP empty vector at bright field; D.tranfected HeLa cells by pEGFP empty vector under fluorescent microscopy at the same field]] |
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+ | [[Image:transfection2.png|800px|center|transfection]] |
Revision as of 14:52, 19 October 2009
Targeted Biobrick Construction
In this section, we were contructing two contructs in paralell. First, standardized biobrick for parts registry, where Targeted Biobrick can be served for further use by other teams or researchers. Second, incorporate Targeted Biobrick into an expression vector pET-28a to test its expression. Targeted Biobrick was contructed via two rounds of PCR.
The expression vector was used for Targeted Biobrick characterization, while the standardized Targeted Biobrick has been submitted to iGEM headquarters for parts registry. We have successfully gained the desired Targeted Biobrick protein of 44184.58 Daltons.