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Team:Tsinghua/Result3
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GuoQiangChen (Talk | contribs) (→Targeted Biobrick Characterization by Fusing with GFP) |
GuoQiangChen (Talk | contribs) (→Targeted Biobrick Construction) |
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[[Image:j44.png|600px|center|j44|thumb|Identification of the Targeted Biobrick positive clones. Desired bands are at 1200-1300 bp]] | [[Image:j44.png|600px|center|j44|thumb|Identification of the Targeted Biobrick positive clones. Desired bands are at 1200-1300 bp]] | ||
| - | [[Image:SDS-PAGE of TB.png|600px|center|thumb|SDS-PAGE of the purified Targeted Biobrick]] | + | The expression vector was used for Targeted Biobrick characterization, while the standardized Targeted Biobrick has been submitted to iGEM headquarters for parts registry. We have successfully gained the desired Targeted Biobrick protein of 44184.58 Daltons. |
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| + | [[Image:SDS-PAGE of TB.png|600px|center|thumb|SDS-PAGE of the purified Targeted Biobrick. The desired Targeted Biobrick protein is 44.2 KD. The left most lane is the marker, where there are bands of 14.4 KD, 20 KD, 30 KD, 43KD, 66 KD and 97 KD respectively]] | ||
=C-Targeted Biobrick Fusion= | =C-Targeted Biobrick Fusion= | ||
Revision as of 14:39, 19 October 2009
Targeted Biobrick Construction
In this section, we were contructing two contructs in paralell. First, standardized biobrick for parts registry, where Targeted Biobrick can be served for further use by other teams or researchers. Second, incorporate Targeted Biobrick into an expression vector pET-28a to test its expression. Targeted Biobrick was contructed via two rounds of PCR.
The expression vector was used for Targeted Biobrick characterization, while the standardized Targeted Biobrick has been submitted to iGEM headquarters for parts registry. We have successfully gained the desired Targeted Biobrick protein of 44184.58 Daltons.
C-Targeted Biobrick Fusion
Targeted Biobrick Characterization by Fusing with GFP
