Team:Tsinghua/Result3
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GuoQiangChen (Talk | contribs) (→Targeted Biobrick Characterization by Fusing with GFP) |
GuoQiangChen (Talk | contribs) (→Targeted Biobrick Characterization by Fusing with GFP) |
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=Targeted Biobrick Characterization by Fusing with GFP= | =Targeted Biobrick Characterization by Fusing with GFP= | ||
+ | ==Targeted Biobrick Production System== | ||
[[Image:transfection.png|900px|center|thumb|Tranfection of pEGFP+Targeted Biobrick into HeLa Cells. A.tranfected HeLa cells by pEGFP+Targeted Biobrick at bright field; B.tranfected HeLa cells by pEGFP+Targeted Biobrick under fluorescent microscopy at the same field; C.control HeLa cells at bright field; D.control HeLa cells under fluorescent microscopy at the same field. 10X10 magnification]] | [[Image:transfection.png|900px|center|thumb|Tranfection of pEGFP+Targeted Biobrick into HeLa Cells. A.tranfected HeLa cells by pEGFP+Targeted Biobrick at bright field; B.tranfected HeLa cells by pEGFP+Targeted Biobrick under fluorescent microscopy at the same field; C.control HeLa cells at bright field; D.control HeLa cells under fluorescent microscopy at the same field. 10X10 magnification]] | ||
[[Image:transfection2.png|900px|center|thumb|Tranfection of pEGFP+Targeted Biobrick or pEGFP empty vector into HeLa Cells. A.tranfected HeLa cells by pEGFP+Targeted Biobrick under fluorescent microscopy; B.tranfected HeLa cells by pEGFP empty vector under fluorescent microscopy. 10X10 magnification]] | [[Image:transfection2.png|900px|center|thumb|Tranfection of pEGFP+Targeted Biobrick or pEGFP empty vector into HeLa Cells. A.tranfected HeLa cells by pEGFP+Targeted Biobrick under fluorescent microscopy; B.tranfected HeLa cells by pEGFP empty vector under fluorescent microscopy. 10X10 magnification]] | ||
+ | ==Targeted Biobrick Characterization System== | ||
[[Image:transfection3.png|900px|center|thumb|Selected Cells for Specificity Testing before co-incubation. A.MRC-5 human diploid fibroblast cells. B.Hela cells. 10X10 magnification]] | [[Image:transfection3.png|900px|center|thumb|Selected Cells for Specificity Testing before co-incubation. A.MRC-5 human diploid fibroblast cells. B.Hela cells. 10X10 magnification]] | ||
Revision as of 15:46, 19 October 2009
Contents |
Targeted Biobrick Construction
In this section, we were contructing two contructs in paralell. First, to contruct a standardized Targeted Biobrick for parts registry, where Targeted Biobrick can be served for further use by other teams or researchers. Second, to incorporate Targeted Biobrick into an expression vector pET-28a to test its expression. Targeted Biobrick was contructed via two rounds of PCR.
The expression vector was used for Targeted Biobrick characterization, while the standardized Targeted Biobrick has been submitted to iGEM headquarters for parts registry. We have successfully gained the desired Targeted Biobrick protein of 44184.58 Daltons.
In conclusion, we have successfully contructed Targeted Biobrick for both part registry and expression (most of the results have not been showed here to make our wiki concise). Of course, the next step should be the characterization of its targeted function.