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Team:UCL London/From the lab/Results
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[[Image:Irongraph.png|500px]] | [[Image:Irongraph.png|500px]] | ||
| - | =Growth Cruves Experiments= | + | =Growth Cruves Experiments I= |
[[Team:UCL_London/From_the_lab/Expts#A_Series_of_Growth_Curve_Experiments_.28OD.26Fluorescent_without_stress.29|'''Link back to Experiment Page''']] | [[Team:UCL_London/From_the_lab/Expts#A_Series_of_Growth_Curve_Experiments_.28OD.26Fluorescent_without_stress.29|'''Link back to Experiment Page''']] | ||
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[[Image:UCL TetR GC.png|700px]]Fig.2b | [[Image:UCL TetR GC.png|700px]]Fig.2b | ||
| + | ===Results=== | ||
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| + | Fig.2a illustrated the growth curves of control (E.coli without any modified plasmids), RPU device ([http://partsregistry.org/Part:BBa_I20260 BBa_I20260]), degP+GFP device ([http://partsregistry.org/Part:BBa_BBa_K239015 BBa_BBa_K239015]), and spy device ([http://partsregistry.org/Part:BBa_BBa_K239009 BBa_K239009]). Fig.2b showed the growth curve of tetR+GFP device ([http://partsregistry.org/Part:BBa_K239012 BBa_K239012]) | ||
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| + | =Growth Cruves Experiments II= | ||
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| + | ===Graphs=== | ||
[[Image:UCL Fluorescent Bar Chart.png|700px]] | [[Image:UCL Fluorescent Bar Chart.png|700px]] | ||
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[[Image:UCL OD&F degP.png|700px]] | [[Image:UCL OD&F degP.png|700px]] | ||
[[Image:UCL OD&F spy.png|700px]] | [[Image:UCL OD&F spy.png|700px]] | ||
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| - | + | ===Results=== | |
</div> | </div> | ||
{{Template:UCL_London_footer}} | {{Template:UCL_London_footer}} | ||
Revision as of 23:08, 21 October 2009
Contents |
Growth Cruves Experiments
Note on Copper
Copper is an essential trace metal for all living organisms. Nevertheless, free intracellular copper is very toxic and has a negative impact on most cells even at very low concentrations. Even though the system for the control of copper levels not is fully understood in E.coli two systems, CueR and CusR, have been identified as regulatory systems for copper homeostasis. According to some researchers, increased levels of extra cellular copper may have the effect of denaturing some membrane proteins. (Yamamoto and Ishihama 2005) This has however not yet been proved so there is still a risk that the CpxAR system could be activated by some additional copper mediated mechanism independent of the presence of misfolded proteins in the periplasm.
Graphs
Growth Cruves Experiments I
Graphs
Fig.2a
Fig.2b
Results
Fig.2a illustrated the growth curves of control (E.coli without any modified plasmids), RPU device (BBa_I20260), degP+GFP device (BBa_BBa_K239015), and spy device (BBa_K239009). Fig.2b showed the growth curve of tetR+GFP device (BBa_K239012)
Growth Cruves Experiments II
Graphs
Results
