Team:UCSF

From 2009.igem.org

(Difference between revisions)
 
(46 intermediate revisions not shown)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+
<!--- The Mission, Experiments --->
-
<html>
+
<html xmlns="http://www.w3.org/1999/xhtml">
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
<head>
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8" />
-
This is a template page. READ THESE INSTRUCTIONS.
+
<title>Untitled Document</title>
-
</div>
+
<style type="text/css">
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
<!--
-
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
+
body {
-
</div>
+
margin-left: 20px;
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
+
margin-right: 20px;
-
You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebookPLEASE keep all of your pages within your teams namespace. 
+
width: 900px;
-
</div>
+
}
-
</div>
+
.style2 {
-
</html>
+
font-size: 30px;
 +
font-weight: bold;
 +
}
 +
-->
 +
</style></head>
 +
<body>
 +
<p align="center"><img src="https://static.igem.org/mediawiki/2009/7/7e/Wiki_2009CellBots.jpg" width="276" height="258" align="middle" /></p>
 +
<br>
 +
<br>
 +
<p  align="center" class="style2">Engineering Motile Cellular Robots</p>
 +
<blockquote>
 +
  <br>
 +
      <h2 align="left">Abstract</h2>
 +
      <p align="left">Some eukaryotic cells, such as white blood cells, have the amazing ability to sense specific external chemical signals and move toward those signals.  This behavior, known as chemotaxis, is a fundamental biological process crucial to such diverse functions as development, wound healing and immune responseIn our project, we used a synthetic biology approach to manipulate signaling pathways that mediate chemotaxis in two model organisms:<br> HL-60 (neutrophil-like) cells and the slime mold, Dictyostelium discoideum. </p>
-
<!-- *** End of the alert box *** -->
+
<p align="left">In doing so, <strong>we have demonstrated that we can regulate both the navigation and speed of our cells, as well as harness their ability to carry a payload.</strong></p>
-
[[Image:Igembanner2 copy.jpg|center]]
+
<p align="left">Through our manipulations, we hope to better understand how these systems work, and eventually to build or reprogram cells that can perform useful tasks. Imagine, for example, therapeutic nanorobots that could home to a directed site in the body and execute complex, user-defined functions (e.g., kill tumors, deliver drugs, guide stem cell migration and differentiation).  Alternatively, imagine bioremediation nanorobots that could find and retrieve toxic substances.  Such cellular robots could be revolutionary biotechnological tools.</p>
 +
    <p align="right"><a href="https://2009.igem.org/Team:UCSF/Project">More...</a></p>
 +
      <p align="right">&nbsp;</p>
 +
      <table width="870" border="0" cellpadding="3">
 +
        <tr>
 +
          <td width="202" height="208"><img src="https://static.igem.org/mediawiki/2009/0/02/Wiki_2009project.jpg" alt="" width="240" height="224" /></td>
 +
          <td width="650"><h3>&nbsp;</h3>
 +
            <blockquote>
 +
              <h3>BUILDING CELL-BOTS</h3>
 +
              <blockquote>
 +
                <h4><a href="https://2009.igem.org/Team:UCSF/Project">Introduction</a></h4>
 +
                <h4><a href="https://2009.igem.org/Team:UCSF/Navigation">Step 1 - Engineering NAVIGATION</a></h4>
 +
                <ul>
 +
                  <ul>
 +
                    <li><a href="https://2009.igem.org/Team:UCSF/Navigation">Inserting New Sensors</a></li>
 +
                    <li><a href="https://2009.igem.org/Team:UCSF/NavigationPart2">Tuning Sensor Sensitivity</a></li>
 +
                  </ul>
 +
                </ul>
 +
                <h4><a href="https://2009.igem.org/Team:UCSF/SPEED">Step 2 - Engineering SPEED</a></h4>
 +
                <h4><a href="https://2009.igem.org/Team:UCSF/PAYLOAD">Step 3 - Carrying a PAYLOAD</a></h4>
 +
                <h4><a href="https://2009.igem.org/Team:UCSF/Future Applications">Our Vision for the Future</a></h4>
 +
              </blockquote>
 +
            </blockquote>
 +
          <p>&nbsp;</p></td>
 +
        </tr>
 +
      </table>
 +
      <table width="870" border="0" cellpadding="3">
 +
        <tr>
 +
          <td width="202" height="208"><img src="https://static.igem.org/mediawiki/2009/d/db/Wiki_2009Team.jpg" alt="" width="240" height="224" /></td>
 +
          <td width="650"><h3>&nbsp;</h3>
 +
              <blockquote>
 +
                <h3>OUR TEAM</h3>
 +
                <blockquote>
 +
                  <h4><a href="https://2009.igem.org/Team:UCSF/Team">Team Members</a></h4>
 +
                  <h4><a href="https://2009.igem.org/Team:UCSF/Notebook">Notebooks</a></h4>
 +
                  <h4><a href="https://2009.igem.org/Team:UCSF/Our_summer_experience">Summer Experience</a></h4>
 +
                  <h4><a href="https://2009.igem.org/Team:UCSF/Human Practices">Human Practices</a></h4>
 +
                  <h4><a href="http://dspace.mit.edu/handle/1721.1/46721">NEW BIOBRICK Standard RFC28 - Aar1 Cloning System</a></h4>
 +
                  <h4><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=UCSF">Parts submitted to the Registry</a></h4>
 +
                  <h4><a href="https://2009.igem.org/Team:UCSF/Gold Medal Requisites">GOLD MEDAL Requisites</a></h4>
 +
                </blockquote>
 +
              </blockquote>
 +
          <p>&nbsp;</p></td>
 +
        </tr>
 +
      </table>
 +
<br><br></br></br>
 +
  </blockquote>
 +
</body>
 +
</html>
-
{|align="justify"
 
-
|'''Engineering the Movement of Cellular Robots'''
 
-
Some eukaryotic cells, such as white blood cells, have the amazing
+
'''UCSF iGEM 2009 is sponsored by...'''
-
ability to sense specific external chemical signals, and move toward
+
-
those signals.  This behavior, known as chemotaxis, is a fundamental
+
-
biological process crucial to such diverse functions as development,
+
-
wound healing and immune response. Our project focuses on using a
+
-
synthetic biology approach to manipulate signaling pathways that mediate
+
-
chemotaxis in two model organisms: HL-60 (neutrophil-like) cells and the
+
-
slime mold, Dictyostelium discoideum. We are attempting to reprogram
+
-
the movements that the cells undergo by altering the guidance and
+
-
movement machinery of these cells in a modular way. For example, can we
+
-
make cells move faster?  Slower?  Can we steer them to migrate toward
+
-
new signals?
+
-
Through our manipulations, we hope to better understand how these
 
-
systems work, and eventually to build or reprogram cells that can
 
-
perform  useful tasks. Imagine, for example, therapeutic nanorobots that
 
-
could home to a directed site in the body and execute complex,
 
-
user-defined functions (e.g., kill tumors, deliver drugs, guide stem
 
-
cell migration and differentiation).  Alternatively, imagine
 
-
bioremediation nanorobots that could find and retrieve toxic
 
-
substances.  Such cellular robots could be revolutionary
 
-
biotechnological tools.
 
-
|[[Image:ucsf3_copy.jpg|200px|right|frame|iGEM Team 2009]]
 
-
|-
 
-
|
 
-
''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
-
|[[Image:Team.png|right|frame|Your team picture]]
 
-
|-
 
-
|
 
-
|align="center"|[[Team:UCSF | Team Example]]
 
-
|}
 
-
<!--- The Mission, Experiments --->
 
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
<html>
-
!align="center"|[[Team:UCSF|Home]]
+
<p align="center"><img src="https://static.igem.org/mediawiki/2009/8/85/Wiki_2009Sponsors.jpg" width="690" height="419" align="middle" /></p></html>
-
!align="center"|[[Team:UCSF/Team|The Team]]
+
-
!align="center"|[[Team:UCSF/Project|The Project]]
+
-
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:UCSF/Modeling|Our summer experience]]
+
-
!align="center"|[[Team:UCSF/Notebook|Notebook]]
+
-
|}
+
-
(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
+

Latest revision as of 01:42, 22 October 2009


Untitled Document



Engineering Motile Cellular Robots


Abstract

Some eukaryotic cells, such as white blood cells, have the amazing ability to sense specific external chemical signals and move toward those signals. This behavior, known as chemotaxis, is a fundamental biological process crucial to such diverse functions as development, wound healing and immune response. In our project, we used a synthetic biology approach to manipulate signaling pathways that mediate chemotaxis in two model organisms:
HL-60 (neutrophil-like) cells and the slime mold, Dictyostelium discoideum.

In doing so, we have demonstrated that we can regulate both the navigation and speed of our cells, as well as harness their ability to carry a payload.

Through our manipulations, we hope to better understand how these systems work, and eventually to build or reprogram cells that can perform useful tasks. Imagine, for example, therapeutic nanorobots that could home to a directed site in the body and execute complex, user-defined functions (e.g., kill tumors, deliver drugs, guide stem cell migration and differentiation). Alternatively, imagine bioremediation nanorobots that could find and retrieve toxic substances. Such cellular robots could be revolutionary biotechnological tools.

More...

 

 

BUILDING CELL-BOTS

Introduction

Step 1 - Engineering NAVIGATION

Step 2 - Engineering SPEED

Step 3 - Carrying a PAYLOAD

Our Vision for the Future

 

 

OUR TEAM

Team Members

Notebooks

Summer Experience

Human Practices

NEW BIOBRICK Standard RFC28 - Aar1 Cloning System

Parts submitted to the Registry

GOLD MEDAL Requisites

 






UCSF iGEM 2009 is sponsored by...