Team:UCSF/Notebook

(Difference between revisions)

Revision as of 17:02, 12 October 2009

tuning receptor sensitivity - Forced localization of effector proteins to GPCRs

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Engineering brakes and accelerators:

workflow:

1. PCR parts: catalytic and localization domains
2. Create combinations of localization domains and catalytic domains
3. Generate Dictyostelium strains expressing feedback elements
4. Measure motility parameters (speed, directionality)
3. Analyze data. Create histograms and compare if our plasmid had any effect to dicty.Do they cause them to move faster or slower?

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Allen Cai's notebook:
Alex Smith's notebook:
Edna Miao's notebook: Transformed new constructs into Dicty, analyzed data, and worked with PTEN null dicty.
Ethan Chan's notebook: Constructed Dicty cells with new parts, analyzed data, and worked with pTen mutated dicty cells.
Ryan Quan's notebook:

@@ Line 13: / Line 13: @@
 == '''Dictyostelium Group''' ==
-* Main Goals:
+* '''Main Goals:'''
-*1. Design brakes, accelerators, steering wheels for Dictyostelium by modulating Pip3 in the cell.
+''Engineering brakes and accelerators:''
-*2. PCR new parts including catalytic and localization domains
+* ''modulating PIP3 polarity by building synthetic protein based feedback loops''
-*3. Create combinations of localization domains and catalytic domains
+workflow:
-*4. Analyze data. Create histograms and compare if our plasmid had any effect to dicty. Ex. cause it to move faster or slower.
+*1. PCR parts: catalytic and localization domains
+*2. Create combinations of localization domains and catalytic domains
+*3. Generate Dictyostelium strains expressing feedback elements
+*4. Measure motility parameters (speed, directionality)
+*3. Analyze data. Create histograms and compare if our plasmid had any effect to dicty.Do they cause them to move faster or slower?
+*
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