"
Team:UCSF/Notebook
From 2009.igem.org
(Difference between revisions)
(→Dictyostelium Group) |
(→Dictyostelium Group) |
||
| Line 27: | Line 27: | ||
*[[Allen Cai's notebook]]: | *[[Allen Cai's notebook]]: | ||
*[[Alex Smith's notebook]]: | *[[Alex Smith's notebook]]: | ||
| - | *[[Edna Miao's notebook]]: Transformed new constructs into Dicty, analyzed data, and worked with PTEN null dicty. | + | *[[Edna Miao's notebook]]: Transformed new constructs into Dicty, analyzed data, and worked with wildtype and PTEN null dicty. |
| - | *[[Ethan Chan's notebook]]: Constructed Dicty cells with new parts, analyzed data, and worked with | + | *[[Ethan Chan's notebook]]: Constructed Dicty cells with new parts, analyzed data, and worked with wildtype and PTEN null dicty cells. |
*[[Ryan Quan's notebook]]: | *[[Ryan Quan's notebook]]: | ||
Revision as of 17:04, 12 October 2009
HL60 Group
- Main Goals:
- Engineering Navigation:
- sending cells to new targets - GPCR Screening
- tuning receptor sensitivity - Forced localization of effector proteins to GPCRs
>
Dictyostelium Group
- Main Goals:
- Engineering brakes and accelerators:
- modulating PIP3 polarity by building synthetic protein based feedback loops
- workflow:
- 1. PCR parts: catalytic and localization domains
- 2. Create combinations of localization domains and catalytic domains
- 3. Generate Dictyostelium strains expressing feedback elements
- 4. Measure motility parameters (speed, directionality)
- 3. Analyze data. Create histograms and compare if our plasmid had any effect to dicty.Do they cause them to move faster or slower?
>
- Allen Cai's notebook:
- Alex Smith's notebook:
- Edna Miao's notebook: Transformed new constructs into Dicty, analyzed data, and worked with wildtype and PTEN null dicty.
- Ethan Chan's notebook: Constructed Dicty cells with new parts, analyzed data, and worked with wildtype and PTEN null dicty cells.
- Ryan Quan's notebook:
