Team:UCSF/Notebook

From 2009.igem.org

(Difference between revisions)
(HL60 Group)
(Dictyostelium Group)
Line 14: Line 14:
== '''Dictyostelium Group''' ==
== '''Dictyostelium Group''' ==
-
* '''Main Goals:'''  
+
* '''Goals:'''  
-
* ''Engineering brakes and accelerators:''  
+
* '''''Engineering brakes and accelerators:'''''  
* ''modulating PIP3 polarity by building synthetic protein based feedback loops''
* ''modulating PIP3 polarity by building synthetic protein based feedback loops''
* workflow:
* workflow:

Revision as of 17:08, 12 October 2009

HL60 Group

  • Goals:
  • Engineering Navigation:
  • sending cells to new targets - GPCR Screening
  • tuning receptor sensitivity - Forced localization of effector proteins to GPCRs
  • Payload:
  • can we make our cells carry stuff?

>

Dictyostelium Group

  • Goals:
  • Engineering brakes and accelerators:
  • modulating PIP3 polarity by building synthetic protein based feedback loops
  • workflow:
  • 1. PCR parts: catalytic and localization domains
  • 2. Create combinations of localization domains and catalytic domains
  • 3. Generate Dictyostelium strains expressing feedback elements
  • 4. Measure motility parameters (speed, directionality)
  • 3. Analyze data. Create histograms and compare if our plasmid had any effect to dicty.Do they cause them to move faster or slower?

>