Team:UCSF/Notebook

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HL60 Group

  • Main Goals:
  • Engineering Navigation:
  • sending cells to new targets - GPCR Screening
  • tuning receptor sensitivity - Forced localization of effector proteins to GPCRs

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Dictyostelium Group

  • Main Goals:
  • Engineering brakes and accelerators:
  • modulating PIP3 polarity by building synthetic protein based feedback loops
  • workflow:
  • 1. PCR parts: catalytic and localization domains
  • 2. Create combinations of localization domains and catalytic domains
  • 3. Generate Dictyostelium strains expressing feedback elements
  • 4. Measure motility parameters (speed, directionality)
  • 3. Analyze data. Create histograms and compare if our plasmid had any effect to dicty.Do they cause them to move faster or slower?

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