Team:UCSF/Notebook

From 2009.igem.org

Revision as of 17:21, 12 October 2009 by OHoeller (Talk | contribs)

HL60 Group

  • Goals:
  • Engineering Navigation:
  • sending cells to new targets - GPCR Screening
  • tuning receptor sensitivity - Forced localization of effector proteins to GPCRs
  • Payload:
  • can we make our cells carry stuff? - attach beads to cells

>

Dictyostelium Group

  • Goals:
  • Engineering brakes and accelerators:
  • modulating PIP3 polarity by building synthetic protein based feedback loops
  • workflow:
  • 1. PCR parts: catalytic and localization domains
  • 2. Create combinations of localization domains and catalytic domains
  • 3. Generate Dictyostelium strains expressing feedback elements
  • 4. Measure motility parameters (speed, directionality)
  • 3. Analyze data. Create histograms and compare if our plasmid had any effect to dicty.Do they cause them to move faster or slower?

>