Team:UC Davis

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every 133 Americans suffer from an autoimmune disorder called Celiac
every 133 Americans suffer from an autoimmune disorder called Celiac
Disease
Disease
-
(14, 11). Celiac Disease is a condition where the small intestines of
+
(14, 11). Celiac Disease is a condition in which the small intestines of
an
an
-
affected individual cannot properly digest gliadin, a protein present in gluten.
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affected individual cannot properly digest gliadin, a protein present
 +
in gluten.
When
When
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consumed, gliadin induces immune system responses, whose
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consumed, gliadin induces immune system responses, the
-
side-effects are,
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side-effects of which are,
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but not limited to abdominal pain, chronic diarrhea and vomiting (14).
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but not limited to: abdominal pain, chronic diarrhea, and vomiting (14).
-
 
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Currently, the only accepted treatment for this disorder is adhering to
Currently, the only accepted treatment for this disorder is adhering to
a
a
gluten-free diet (14, 12). This can be very difficult as great
gluten-free diet (14, 12). This can be very difficult as great
varieties of
varieties of
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food contain gluten. This inspired us to imagine an alternatives
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food contain gluten. This inspired us to imagine an alternative
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treatment base on a probiotic organism
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treatment based on a probiotic organism
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that could
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that can
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survive and take residence in the stomach where it would secrete an
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survive and take residence in the stomach, where it would secrete an
enzyme that
enzyme that
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can degrade gliadin.</big></span><big style="font-weight: bold;"><span
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degrades gliadin.</big></span><big style="font-weight: bold;"><span
style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><br>
style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><br>
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp;&nbsp; Then the design
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp;&nbsp; Then the design
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elements of this organism should be able to satisfy at least two main
+
elements of this organism should satisfy at least two main
criteria.
criteria.
First, it
First, it
-
should be able to survive ONLY in the stomach and, secondly, secrete an
+
should be able to survive only in the stomach; second, it should secrete an
enzyme
enzyme
-
that can degrade gliadin<i>.</i> We envision that these parts would not
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that degrades gliadin<i>.</i> We envision that these parts would
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only be useful for our purpose probiotic, but could be useful to number
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not
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of other projects.</span><span
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only be useful for our project, but also to other future projects.</span><span
style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span><br>
style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span><br>
</big><span style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big
</big><span style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big
style="font-weight: bold;">&nbsp;&nbsp;&nbsp;
style="font-weight: bold;">&nbsp;&nbsp;&nbsp;
&nbsp; &nbsp;
&nbsp; &nbsp;
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&nbsp;&nbsp; We have
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&nbsp;&nbsp; We decided to focus our summer project on building the
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decided to focus our summer project on building two key elements
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first of the two key elements
required for
required for
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our “dream bug”. Therefore, our project has been split into two tasks:<o:p></o:p><br>
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our system:<o:p></o:p><br>
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; 1. <a
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; 1. <a
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biological pH sensor limiting this secretion system to
biological pH sensor limiting this secretion system to
the
the
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stomach</a><br>
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stomach</a></big></span><a
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href="https://2009.igem.org/Team:UC_Davis/ph_sensor1"><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><span
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style="text-decoration: underline;"></span></span></a><span
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&nbsp;</span><a href="https://2009.igem.org/Team:UC_Davis/ph_sensor1"><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><span
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style="text-decoration: underline;"></span></span></a><a
href="https://2009.igem.org/Team:UC_Davis/Project_Summary"><img alt=""
href="https://2009.igem.org/Team:UC_Davis/Project_Summary"><img alt=""
src="https://static.igem.org/mediawiki/2009/a/ac/UCDAVIS_summary.png"
src="https://static.igem.org/mediawiki/2009/a/ac/UCDAVIS_summary.png"
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</span> </span><a href="https://2009.igem.org/Team:UC_Davis/ph_sensor1"><b
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style="width: 182px; height: 108px;" alt=""
src="https://static.igem.org/mediawiki/2009/c/c6/UCDAVIS_genome_center.jpg"><br>
src="https://static.igem.org/mediawiki/2009/c/c6/UCDAVIS_genome_center.jpg"><br>
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<font size="-1">Special thanks to <span style="font-weight: bold;">Suzy
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<font size="-1">We want to thank <span style="font-weight: bold;">Marc</span>
 +
<span style="font-weight: bold;">Facciotti</span> for his helpful
 +
advice.<br>
 +
Special thanks to <span style="font-weight: bold;">Suzy
Fenton</span>,<span style="font-weight: bold;">Wayne Fenton, Jeanne
Fenton</span>,<span style="font-weight: bold;">Wayne Fenton, Jeanne
Joe-Fenton </span>and<span style="font-weight: bold;"> Wayne Fenton Jr.</span>
Joe-Fenton </span>and<span style="font-weight: bold;"> Wayne Fenton Jr.</span>

Latest revision as of 18:07, 21 October 2009

Homepage_UCdavis

     

              It is estimated that about one in every 133 Americans suffer from an autoimmune disorder called Celiac Disease (14, 11). Celiac Disease is a condition in which the small intestines of an affected individual cannot properly digest gliadin, a protein present in gluten. When consumed, gliadin induces immune system responses, the side-effects of which are, but not limited to: abdominal pain, chronic diarrhea, and vomiting (14). Currently, the only accepted treatment for this disorder is adhering to a gluten-free diet (14, 12). This can be very difficult as great varieties of food contain gluten. This inspired us to imagine an alternative treatment based on a probiotic organism that can survive and take residence in the stomach, where it would secrete an enzyme that degrades gliadin.
           Then the design elements of this organism should satisfy at least two main criteria. First, it should be able to survive only in the stomach; second, it should secrete an enzyme that degrades gliadin. We envision that these parts would not only be useful for our project, but also to other future projects.

           We decided to focus our summer project on building the first of the two key elements required for our system:
                1. Produce an inducible secretion system
                2. Construct a biological pH sensor limiting this secretion system to the stomach

                                                                                                                                                                                                                                                                                                                                  


      UC Davis iGEM team is sponsored by:
     
We want to thank Marc Facciotti for his helpful advice.
Special thanks to Suzy Fenton,Wayne Fenton, Jeanne Joe-Fenton and Wayne Fenton Jr.
Also we thank Charles H. Halsted for reviewing our material regarding celiac disease.