Team:UC Davis

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<div style="text-align: center;"><span
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style="color: rgb(255, 255, 153);">&nbsp;
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</span><b style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></b><span
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style="color: rgb(255, 255, 153);"><img src="" alt=""
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style="width: 151px; height: 161px;"></span><b
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style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></b><b
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style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></b><a
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href="https://2009.igem.org/Team:UC_Davis/About_Us"><b
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style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></b></a><b
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style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></b><a
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href="https://2009.igem.org/Team:UC_Davis/About_Us"><b
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style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></b></a><b
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style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></b><b
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style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big>&nbsp;
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</big></big></big></big></span></b><b style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><a
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href="https://2009.igem.org/Team:UC_Davis/About_Us"><img alt=""
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src="https://static.igem.org/mediawiki/2009/d/d1/UCDAVIS_PIC4.png"
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style="border: 0px solid ; width: 92px; height: 46px;"></a> </big></big></big></big></span></b><b
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style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><a
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href="https://2009.igem.org/Team:UC_Davis/Project"><img alt=""
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src="https://static.igem.org/mediawiki/2009/b/b9/UCDAVIS_PIC8.png"
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style="border: 0px solid ; width: 92px; height: 46px;"></a> </big></big></big></big></span></b><b
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style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><a
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href="https://2009.igem.org/Team:UC_Davis/Notebook"><img alt=""
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src="https://static.igem.org/mediawiki/2009/2/2f/UCDAVIS_PIC5.png"
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style="border: 0px solid ; width: 92px; height: 46px;"></a> </big></big></big></big></span></b><b
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style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></b><b
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style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><a
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href="https://2009.igem.org/Team:UC_Davis/Parts"><img alt=""
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src="https://static.igem.org/mediawiki/2009/a/a6/UCDAVIS_PIC6.png"
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style="border: 0px solid ; width: 92px; height: 46px;"></a>&nbsp;<a
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href="https://2009.igem.org/Team:UC_Davis/Contacts_References"><img
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alt="" src="https://static.igem.org/mediawiki/2009/1/1d/UCDAVIS_PIC7.png"
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style="border: 0px solid ; width: 92px; height: 46px;"></a></big></big></big></big></span></b><b
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style="color: rgb(0, 0, 0);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><small>
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<img style="width: 191px; height: 147px;" alt="" src=""><br>
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</small></big></big></big></big></span></b></div>
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<hr style="width: 100%; height: 2px;"><b style="color: rgb(0, 0, 0);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></b><big><span
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style="color: rgb(0, 0, 0);"></span></big><b
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style="color: rgb(0, 0, 0);"><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><span
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style="font-family: Symbol;"></span><span
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style="font-family: Arial Narrow; font-weight: bold;"></span></span></b><span
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style="color: rgb(0, 0, 0); font-family: Arial Narrow; font-weight: bold;"><span
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style="font-size: 13pt; line-height: 115%;"></span></span>
<p class="MsoNormal"
<p class="MsoNormal"
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style="margin-bottom: 0.0001pt; line-height: normal; color: rgb(255, 255, 255); text-align: left;"><b><span
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style="margin-bottom: 0.0001pt; line-height: normal;"><b><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><br>
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">&nbsp;&nbsp;&nbsp;
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</span></b></p>
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&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;<big> </big></span></b><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black; font-weight: bold;"><big>&nbsp;
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</big><big>It
 +
is estimated that about one in
 +
every 133 Americans suffer from an autoimmune disorder called Celiac
 +
Disease
 +
(14, 11). Celiac Disease is a condition in which the small intestines of
 +
an
 +
affected individual cannot properly digest gliadin, a protein present
 +
in gluten.
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When
 +
consumed, gliadin induces immune system responses, the
 +
side-effects of which are,
 +
but not limited to: abdominal pain, chronic diarrhea, and vomiting (14).
 +
Currently, the only accepted treatment for this disorder is adhering to
 +
a
 +
gluten-free diet (14, 12). This can be very difficult as great
 +
varieties of
 +
food contain gluten. This inspired us to imagine an alternative
 +
treatment based on a probiotic organism
 +
that can
 +
survive and take residence in the stomach, where it would secrete an
 +
enzyme that
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degrades gliadin.</big></span><big style="font-weight: bold;"><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><br>
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&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp;&nbsp; Then the design
 +
elements of this organism should satisfy at least two main
 +
criteria.
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First, it
 +
should be able to survive only in the stomach; second, it should secrete an
 +
enzyme
 +
that degrades gliadin<i>.</i> We envision that these parts would
 +
not
 +
only be useful for our project, but also to other future projects.</span><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span><br>
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</big><span style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big
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style="font-weight: bold;">&nbsp;&nbsp;&nbsp;
 +
&nbsp; &nbsp;
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&nbsp;&nbsp; We decided to focus our summer project on building the
 +
first of the two key elements
 +
required for
 +
our system:<o:p></o:p><br>
 +
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;
 +
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; 1. <a
 +
href="https://2009.igem.org/Team:UC_Davis/secretion">Produce an
 +
inducible secretion
 +
system</a><br>
 +
</big>
 +
<big style="font-weight: bold;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
2. <a href="https://2009.igem.org/Team:UC_Davis/ph_sensor1">Construct a
 +
biological pH sensor limiting this secretion system to
 +
the
 +
stomach</a></big></span><a
 +
href="https://2009.igem.org/Team:UC_Davis/ph_sensor1"><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><span
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style="text-decoration: underline;"></span></span></a><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big
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style="font-weight: bold;"><br>
 +
</big></span></p>
<p class="MsoNormal"
<p class="MsoNormal"
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style="margin-bottom: 0.0001pt; line-height: normal; color: rgb(255, 255, 255); text-align: center;"><b><span
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style="margin-bottom: 0.0001pt; line-height: normal;"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><br>
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span><a
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<big>Project and team descriptions:</big></span></b><span
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href="https://2009.igem.org/Team:UC_Davis/ph_sensor1"><b
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style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
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style="color: rgb(0, 0, 0);"><span
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<div style="text-align: center;"></div>
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></b></a><span
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<div style="text-align: center;"></div>
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;
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&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;
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&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;
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&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;
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&nbsp; &nbsp; &nbsp; &nbsp; &nbsp;
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp;</span><a href="https://2009.igem.org/Team:UC_Davis/ph_sensor1"><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><span
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style="text-decoration: underline;"></span></span></a><a
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href="https://2009.igem.org/Team:UC_Davis/Project_Summary"><img alt=""
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src="https://static.igem.org/mediawiki/2009/a/ac/UCDAVIS_summary.png"
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style="border: 0px solid ; width: 63px; height: 50px;"></a><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<span style="text-decoration: underline;"><br>
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</span> </span><a href="https://2009.igem.org/Team:UC_Davis/ph_sensor1"><b
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style="color: rgb(0, 0, 0);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></b></a><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span><b
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style="color: rgb(0, 0, 0);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></b></p>
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<hr style="width: 100%; height: 2px;">
<p class="MsoNormal"
<p class="MsoNormal"
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style="margin-bottom: 0.0001pt; line-height: normal; color: rgb(255, 255, 255); text-align: center;"><b><span
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style="margin-bottom: 0.0001pt; line-height: normal; color: rgb(0, 0, 0); text-align: center; background-color: rgb(255, 255, 255);"><b><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">&nbsp;</span></b><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></b><b><span
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style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span><br>
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></b><b><span
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<b><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></b><b><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></b></p>
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1 in 133 Americans are suffering
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<ul style="background-color: rgb(255, 255, 255); color: rgb(0, 0, 0);">
-
from a form of autoimmune disorder called Celiac Disease. Celiac
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</ul>
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Disease is an
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<div style="text-align: center;"><big> </big></div>
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condition where the small intestines of an affected individual cannot
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<div style="text-align: center;">
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digest
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<div style="text-align: left;">
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gliadin, a protein present in gluten. If consumed, the body kick starts
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<div style="text-align: center;"><big><span style="font-weight: bold;"><big>&nbsp;&nbsp;
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its
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&nbsp;&nbsp;<small>
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immune system response. The effects of the immune response include, but
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UC
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are not
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Davis iGEM team is sponsored by:</small></big></span></big><br>
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limited to abdominal pain and vomiting.</span></b><span
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<big><span style="font-weight: bold;"></span></big></div>
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style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
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<div style="text-align: center;">&nbsp;&nbsp; &nbsp;&nbsp; <img
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<div style="text-align: center;"></div>
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src="https://static.igem.org/mediawiki/2009/8/88/UCDAVIS_Biomedical_engineering.jpg"
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alt="" style="width: 83px; height: 109px;"><img
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style="width: 105px; height: 109px;" alt=""
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src="https://static.igem.org/mediawiki/2009/4/43/UCDAVIS_college_of_engineering.png"><img
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style="width: 135px; height: 99px;" alt=""
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src="https://static.igem.org/mediawiki/2009/8/8e/UCDAVIS_Accutek1.jpg"><img
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style="width: 121px; height: 109px;" alt=""
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src="https://static.igem.org/mediawiki/2009/6/6b/UCDAVIS_mrgen.gif"><img
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style="width: 182px; height: 108px;" alt=""
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src="https://static.igem.org/mediawiki/2009/c/c6/UCDAVIS_genome_center.jpg"><br>
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<font size="-1">We want to thank <span style="font-weight: bold;">Marc</span>
 +
<span style="font-weight: bold;">Facciotti</span> for his helpful
 +
advice.<br>
 +
Special thanks to <span style="font-weight: bold;">Suzy
 +
Fenton</span>,<span style="font-weight: bold;">Wayne Fenton, Jeanne
 +
Joe-Fenton </span>and<span style="font-weight: bold;"> Wayne Fenton Jr.</span>
 +
<br>
 +
Also we thank <span style="font-size: 10pt; line-height: 115%;"><b>Charles
 +
H. <span class="il">Halsted </span></b><span class="il">for reviewing
 +
our material regarding celiac disease.</span></span></font><br>
 +
<br>
 +
</div>
 +
</div>
 +
</div>
 +
<ul style="background-color: rgb(255, 255, 255); color: rgb(0, 0, 0);">
 +
<span style="font-weight: bold;"></span>
 +
</ul>
<p class="MsoNormal"
<p class="MsoNormal"
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style="margin-bottom: 0.0001pt; line-height: normal; color: rgb(255, 255, 255); text-align: center;"><b><span
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style="margin-bottom: 0.0001pt; line-height: normal; color: rgb(0, 0, 0); text-align: left; background-color: rgb(255, 255, 255);"><b><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><br>
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; One of our teammate's friends is
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</span></b></p>
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currently suffering from this illness, which
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<span style="color: rgb(0, 0, 0);"></span>
-
prompted us to look into Celiac Disease as our main focus. What we
+
-
learned that currently, no cure has been found and the only treatment
+
-
is a gluton free diet.
+
-
This treatment is very difficult as an overwhelming majority of food
+
-
contain
+
-
gluten and gliadin.</span></b><span
+
-
style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
+
-
<div style="text-align: center;"></div>
+
-
<p class="MsoNormal"
+
-
style="margin-bottom: 0.0001pt; line-height: normal; color: rgb(255, 255, 255); text-align: center;"><b><span
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-
style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">&nbsp;</span></b><span
+
-
style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
+
-
<div style="text-align: center;"></div>
+
-
<p class="MsoNormal"
+
-
style="margin-bottom: 0.0001pt; line-height: normal; color: rgb(255, 255, 255); text-align: center;"><b><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+
-
This year the University of
+
-
California: Davis entered the 2009 iGEM competition a focus on creating
+
-
a new
+
-
way to treat Celiac. Our project has been split into two tasks. The
+
-
first is to
+
-
produce an inducible secretion system; the second is to construct a
+
-
biological
+
-
pH sensor to compartmentalize this secretion system in the stomach.
+
-
Also, this
+
-
pH system will act as a kill switch if this system were to ever leave
+
-
the
+
-
stomach by inducing cell death via pH change. After weeks of reading
+
-
numerous
+
-
articles, we are confident that with the correct parts and timing, our
+
-
two
+
-
systems can be built in time to present to the iGEM community.</span></b><span
+
-
style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
+
-
<div style="text-align: center;"></div>
+
-
<div style="text-align: center;"></div>
+
-
<p class="MsoNormal"
+
-
style="margin-bottom: 0.0001pt; line-height: normal; color: rgb(255, 255, 255); text-align: center;"><span
+
-
style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p>&nbsp;</o:p></span><br>
+
-
<b><span
+
-
style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">We
+
-
have recently moved into the synthesis
+
-
and assembly stage of our two systems.</span></b><span
+
-
style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
+
-
<div style="text-align: center;"></div>
+
-
<div style="text-align: center;"></div>
+
-
<p class="MsoNormal"
+
-
style="margin-bottom: 5pt; line-height: normal; color: rgb(255, 255, 255); text-align: center;"><span
+
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style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p>&nbsp;</o:p></span><b><span
+
-
style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">Though
+
-
this is our first year in the competition, we feel
+
-
that we are moving at a good pace, working out kinks along the way.</span></b><span
+
-
style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
+
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<div style="text-align: center;"></div>
+
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<p style="color: rgb(255, 255, 255); text-align: center;"
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Latest revision as of 18:07, 21 October 2009

Homepage_UCdavis

     

              It is estimated that about one in every 133 Americans suffer from an autoimmune disorder called Celiac Disease (14, 11). Celiac Disease is a condition in which the small intestines of an affected individual cannot properly digest gliadin, a protein present in gluten. When consumed, gliadin induces immune system responses, the side-effects of which are, but not limited to: abdominal pain, chronic diarrhea, and vomiting (14). Currently, the only accepted treatment for this disorder is adhering to a gluten-free diet (14, 12). This can be very difficult as great varieties of food contain gluten. This inspired us to imagine an alternative treatment based on a probiotic organism that can survive and take residence in the stomach, where it would secrete an enzyme that degrades gliadin.
           Then the design elements of this organism should satisfy at least two main criteria. First, it should be able to survive only in the stomach; second, it should secrete an enzyme that degrades gliadin. We envision that these parts would not only be useful for our project, but also to other future projects.
           We decided to focus our summer project on building the first of the two key elements required for our system:
                1. Produce an inducible secretion system
                 2. Construct a biological pH sensor limiting this secretion system to the stomach

                                                                                                                                                                                                                                                                                                                                  

      UC Davis iGEM team is sponsored by:
     
We want to thank Marc Facciotti for his helpful advice.
Special thanks to Suzy Fenton,Wayne Fenton, Jeanne Joe-Fenton and Wayne Fenton Jr.
Also we thank Charles H. Halsted for reviewing our material regarding celiac disease.

                                         

Retrieved from "http://2009.igem.org/Team:UC_Davis"