Team:UC Davis/Adding secretion

From 2009.igem.org

(Difference between revisions)

Revision as of 01:30, 25 September 2009

Adding Secretion

Adding Secretion:

General model for secretion Secretion Models Why test different genes

General model for secretion system:

Advantages of having several models:

In our secretion system, we are using two genes with different sizes as our target secretion genes, GFP being short in length and Luciferase being comparably long would test our secretion system and its ability to secrete small and large proteins.
Testing multiple different combinations; Signal Sequence alone, Signal Sequence plus ompA/INPNC and ompA/INPNC alone, would help us find the best combination for our secretion system based on their secretion ability and rank them from strongest to weakest in strength (in this specific system).

Secretion Models:
Click on a specific model for more information:

Why test different genes in our secretion system?

There is wide range of genes present in our environment. Therefore, it is important to measure our secretion system’s ability to secrete genes of various sizes.

Molecular data on some proteins

	Molecular Weight	Number of residues	Number of polypeptide
Cytochrome c (human)	13,000	104	1
Ribonuclease A(bovine pancreas)	13,700	124	1
Serum albumin (human)	68,500	609	1

(Page 128, third edition “Lehninger Principles of Biochemistry” by David L. Nelson and Michael M. Cox)

@@ Line 123: / Line 123: @@
 alt="" src="https://static.igem.org/mediawiki/2009/b/ba/UCDAVIS_PIC12.png"
 style="border: 0px solid ; width: 95px; height: 63px;"></a><a
-href="UCDAVIS_Model%202.html"><img alt=""
+href="https://2009.igem.org/Team:UC_Davis/Adding_secretion/model_2"><img
-src="https://static.igem.org/mediawiki/2009/a/a4/UCDAVIS_PIC13.png"
+alt="" src="https://static.igem.org/mediawiki/2009/a/a4/UCDAVIS_PIC13.png"
 style="border: 0px solid ; width: 95px; height: 63px;"></a><a
-href="UCDAVIS_Model%203.html"><img alt=""
+href="https://2009.igem.org/Team:UC_Davis/Adding_secretion/model_3"><img
-src="https://static.igem.org/mediawiki/2009/9/9a/UCDAVIS_PIC14.png"
+alt="" src="https://static.igem.org/mediawiki/2009/9/9a/UCDAVIS_PIC14.png"
 style="border: 0px solid ; width: 95px; height: 63px;"></a><a
-href="UCDAVIS_Model%204.html"><img alt=""
+href="https://2009.igem.org/Team:UC_Davis/Adding_secretion/model_4"><img
-src="https://static.igem.org/mediawiki/2009/b/ba/UCDAVIS_PIC15.png"
+alt="" src="https://static.igem.org/mediawiki/2009/b/ba/UCDAVIS_PIC15.png"
 style="border: 0px solid ; width: 95px; height: 63px;"></a><a
-href="UCDAVIS_Model%205.html"><img alt=""
+href="https://2009.igem.org/Team:UC_Davis/Adding_secretion/model_5"><img
-src="https://static.igem.org/mediawiki/2009/5/5d/UCDAVIS_PIC16.png"
+alt="" src="https://static.igem.org/mediawiki/2009/5/5d/UCDAVIS_PIC16.png"
 style="border: 0px solid ; width: 95px; height: 63px;"></a>&nbsp;&nbsp;&nbsp;
 </span><span style="font-style: italic;"><big><span
@@ Line 167: / Line 167: @@
 style="text-decoration: underline;">Molecular data on some proteins</span></big><o:p></o:p></span></b></div>
 <table class="MsoNormalTable"
-style="border: medium none ; border-collapse: collapse; text-align: left; margin-left: auto; margin-right: auto; width: 597px; height: 264px;"
+style="border: medium none ; border-collapse: collapse; text-align: left; margin-left: auto; margin-right: auto; width: 405px; height: 264px;"
 border="1" cellpadding="0" cellspacing="0">
 <tbody>
@@ Line 182: / Line 182: @@
 valign="top" width="157"><small><small> </small></small>
 <p class="MsoNormal"
-style="margin-bottom: 0.0001pt; line-height: normal;"><small><small><b><span
+style="margin-bottom: 0.0001pt; line-height: normal;"><small><small><small><small><b><span
 style="font-size: 14pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">Molecular
-Weight<o:p></o:p></span></b></small></small></p>
+Weight</span></b></small></small></small></small></p>
 <small><small> </small></small></td>
 <td
@@ Line 191: / Line 191: @@
 <p class="MsoNormal"
 style="margin-bottom: 0.0001pt; line-height: normal;"><small><b><span
-style="font-size: 14pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">Number
+style="font-size: 14pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><small>Number
-of residues<o:p></o:p></span></b></small></p>
+of residues</small><o:p></o:p></span></b></small></p>
 <small> </small></td>
 <td
@@ Line 199: / Line 199: @@
 <p class="MsoNormal"
 style="margin-bottom: 0.0001pt; line-height: normal;"><b><span
-style="font-size: 14pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">Number
+style="font-size: 14pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><small><br>
-of polypeptide<o:p></o:p></span></b></p>
+Number
+of polypeptide</small><o:p></o:p></span></b></p>
 </td>
 </tr>
@@ Line 209: / Line 210: @@
 <p class="MsoNormal"
 style="margin-bottom: 0.0001pt; line-height: normal;"><b><span
-style="font-size: 14pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">Cytochrome
+style="font-size: 14pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><small>Cytochrome
-<i>c </i>(human)<o:p></o:p></span></b></p>
+<i>c </i>(human)</small><o:p></o:p></span></b></p>
 </td>
 <td
@@ Line 240: / Line 241: @@
 <p class="MsoNormal"
 style="margin-bottom: 0.0001pt; line-height: normal;"><b><span
-style="font-size: 14pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">Ribonuclease
+style="font-size: 14pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><small>Ribonuclease
-A(bovine pancreas)<o:p></o:p></span></b></p>
+A(bovine pancreas)</small><o:p></o:p></span></b></p>
 </td>
 <td
@@ Line 271: / Line 272: @@
 <p class="MsoNormal"
 style="margin-bottom: 0.0001pt; line-height: normal;"><b><span
-style="font-size: 14pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">Serum
+style="font-size: 14pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><small>Serum
-albumin (human)<o:p></o:p></span></b></p>
+albumin (human)</small><o:p></o:p></span></b></p>
 </td>
 <td