Team:UC Davis/Adding secretion

From 2009.igem.org

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of having several models:</span></b><b><span
of having several models:</span></b><b><span
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<div style="text-align: left;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; The
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style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">&nbsp;&nbsp;&nbsp;
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purpose of the secretion system is to introduce a method
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&nbsp;&nbsp; In
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of excreting target proteins we wish to synthesize in the marvelous
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our secretion system, we are using two genes with different sizes as
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host: E.coli
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our target
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HB101.&nbsp; Through this approach of being able to secrete specific
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secretion genes, GFP being short in length and Luciferase being
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target
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comparably long
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proteins, we hope to serve as a chassis to the rest of the biological
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would test our secretion system and its ability to secrete small and
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world.<br>
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large
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&nbsp;&nbsp;&nbsp; &nbsp;&nbsp; In our secretion system, we are using
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proteins. <br>
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two
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Testing multiple different combinations;
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genes with different sizes as our target secretion genes, GFP being
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Signal
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short in
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Sequence alone, Signal Sequence plus ompA/INPNC and ompA/INPNC alone,
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length and Luciferase being comparably long would test our secretion
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would
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system and
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help us find the best combination for our secretion system based on
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its ability to secrete small and large proteins. <br>
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their
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&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; Testing multiple different
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secretion ability and rank them from strongest to weakest in strength
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combinations; Signal Sequence alone, Signal Sequence plus ompA/INPNC
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(in this
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and
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specific system).</span></p>
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ompA/INPNC alone, would help us find the best combination for our
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<big><big><big><span style="font-weight: bold;"><small><span
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secretion
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system based on their secretion ability and rank them from strongest to
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weakest
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in strength (in this specific system).</div>
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<div style="text-align: left;"><big><big><big><span
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style="font-weight: bold;"><small><span
style="text-decoration: underline; background-color: rgb(255, 255, 0);"></span></small></span></big></big></big><br>
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</div>
<div style="text-align: left;">
<div style="text-align: left;">
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<hr style="width: 100%; height: 2px;"><big><small><big
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style="font-size: 14pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><small><small>Number
style="font-size: 14pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><small><small>Number
of polypeptide</small></small><o:p></o:p></span></b></small></p>
of polypeptide</small></small><o:p></o:p></span></b></small></p>
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style="margin-bottom: 0.0001pt; line-height: normal;"><b><span
style="margin-bottom: 0.0001pt; line-height: normal;"><b><span
style="font-size: 14pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><small><small>Cytochrome
style="font-size: 14pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><small><small>Cytochrome
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<i>c </i>(human</small></small></span></b></p>
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<i>c </i>(human )<br>
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</small></small></span></b></p>
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style="font-size: 14pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><small>13,000</small><o:p></o:p></span></b></small></small></p>
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style="font-size: 14pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><small>13,000</small></span></b></small></small></p>
<small><small> </small></small></td>
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style="margin-bottom: 0.0001pt; line-height: normal;"><small><b><span
style="font-size: 14pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><small>104</small><o:p></o:p></span></b></small></p>
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&nbsp; 1</small><o:p></o:p></span></b></p>
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&nbsp; 1</small><o:p></o:p></span></b></p>
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609</small><o:p></o:p></span></b></p>
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&nbsp; 1</small><o:p></o:p></span></b></p>
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Revision as of 03:19, 13 October 2009

Adding Secretion

 
Adding Secretion:
General model for secretion     Secretion Models     Why test different genes

General model for secretion system:

Advantages of having several models:

      The purpose of the secretion system is to introduce a method of excreting target proteins we wish to synthesize in the marvelous host: E.coli HB101.  Through this approach of being able to secrete specific target proteins, we hope to serve as a chassis to the rest of the biological world.
       In our secretion system, we are using two genes with different sizes as our target secretion genes, GFP being short in length and Luciferase being comparably long would test our secretion system and its ability to secrete small and large proteins.
        Testing multiple different combinations; Signal Sequence alone, Signal Sequence plus ompA/INPNC and ompA/INPNC alone, would help us find the best combination for our secretion system based on their secretion ability and rank them from strongest to weakest in strength (in this specific system).

Secretion Models:
Click on a specific model for more information:
   

Why test different genes in our secretion system?

     There is wide range of genes present in our environment. Therefore, it is important to measure our secretion system’s ability to secrete genes of various sizes.

Molecular data on some proteins

 

Name

Molecular
Weight

Number of residues

Number of polypeptide

Cytochrome c (human )

13,000

104

 
  1

Ribonuclease A(bovine pancreas)


13,700


124


  1

Serum albumin (human)


68,500


609

 
  1

(Page 128, third edition “Lehninger Principles of Biochemistry” by David L. Nelson and Michael M. Cox)




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