Team:UC Davis/Adding secretion

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href="https://2009.igem.org/Team:UC_Davis/homepage2"><img alt="" src="" style="border: 0px solid ; width: 83px; height: 36px;"></a> <a href="https://2009.igem.org/Team:UC_Davis/About_Us1"><img alt="" src="" style="border: 0px solid ; width: 82px; height: 36px;"></a> <a href="https://2009.igem.org/Team:UC_Davis/Project1"><img alt="" src="" style="border: 0px solid ; width: 78px; height: 36px;"></a> <a href="https://2009.igem.org/Team:UC_Davis/Notebook1"><img alt="" src="" style="border: 0px solid ; width: 81px; height: 36px;"></a> <a href="https://2009.igem.org/Team:UC_Davis/Part1"><img alt="" src="" style="border: 0px solid ; width: 78px; height: 37px;"></a> <a href="https://2009.igem.org/Team:UC_Davis/Contact1"><img alt="" src=""

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Adding Secretion:

<a

href="#general_model">General model for secretion</a> <a href="#secretion_model">Secretion Models</a> <a href="#different_genes">Why test different genes</a>

<o:p></o:p>

<a

name="general_model"></a>General model for secretion system:
<img style="width: 242px; height: 58px; background-color: rgb(255, 255, 255);" alt="" src=""><img style="width: 89px; height: 81px; background-color: rgb(255, 255, 255);" alt="" src=""><img style="width: 106px; height: 56px; background-color: rgb(255, 255, 255);" alt="" src=""><img style="width: 94px; height: 69px; background-color: rgb(255, 255, 255);" alt="" src=""><img style="width: 106px; height: 59px; background-color: rgb(255, 255, 255);" alt="" src=""><img style="width: 134px; height: 66px; background-color: rgb(255, 255, 255);" alt="" src=""><img style="width: 94px; height: 83px; background-color: rgb(255, 255, 255);" alt="" src="">

Advantages of having several models:<o:p></o:p>

In our secretion system, we are using two genes with different sizes as our target secretion genes, GFP being short in length and Luciferase being comparably long would test our secretion system and its ability to secrete small and large proteins.
Testing multiple different combinations; Signal Sequence alone, Signal Sequence plus ompA/INPNC and ompA/INPNC alone, would help us find the best combination for our secretion system based on their secretion ability and rank them from strongest to weakest in strength (in this specific system).

<a

name="secretion_model"></a>Secretion Models:
Click on a specific model for more information:

<a

href="https://2009.igem.org/Team:UC_Davis/Adding_secretion/model_1"><img alt="" src="" style="border: 0px solid ; width: 95px; height: 63px;"></a><a href="UCDAVIS_Model%202.html"><img alt="" src="" style="border: 0px solid ; width: 95px; height: 63px;"></a><a href="UCDAVIS_Model%203.html"><img alt="" src="" style="border: 0px solid ; width: 95px; height: 63px;"></a><a href="UCDAVIS_Model%204.html"><img alt="" src="" style="border: 0px solid ; width: 95px; height: 63px;"></a><a href="UCDAVIS_Model%205.html"><img alt="" src="" style="border: 0px solid ; width: 95px; height: 63px;"></a>

<o:p></o:p>

<a
name="different_genes"></a>Why
test different genes in our secretion system?<o:p></o:p>

There is wide range of genes present in our environment. Therefore, it is important to measure our secretion system’s ability to secrete genes of various sizes.

Molecular data on some proteins<o:p></o:p>

<o:p> </o:p>	Molecular Weight<o:p></o:p>	Number of residues<o:p></o:p>	Number of polypeptide<o:p></o:p>
Cytochrome c (human)<o:p></o:p>	13,000<o:p></o:p>	104<o:p></o:p>	1<o:p></o:p>
Ribonuclease A(bovine pancreas)<o:p></o:p>	13,700<o:p></o:p>	124<o:p></o:p>	1<o:p></o:p>
Serum albumin (human)<o:p></o:p>	68,500<o:p></o:p>	609<o:p></o:p>	1<o:p></o:p>

(Page 128, third edition “Lehninger Principles of Biochemistry” by David L. Nelson and Michael M. Cox)<o:p>
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