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Team:UC Davis/Adding secretion
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style="border: 0px solid ; width: 83px; height: 37px;"></a>href="#general_model">General model for secretion</a>
<a href="#secretion_model">Secretion Models</a>
<a href="#different_genes">Why test different genes</a>
<o:p></o:p>
name="general_model"></a>General
model for secretion system:
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Advantages of having several models:<o:p></o:p>
In
our secretion system, we are using two genes with different sizes as
our target
secretion genes, GFP being short in length and Luciferase being
comparably long
would test our secretion system and its ability to secrete small and
large
proteins.
Testing multiple different combinations;
Signal
Sequence alone, Signal Sequence plus ompA/INPNC and ompA/INPNC alone,
would
help us find the best combination for our secretion system based on
their
secretion ability and rank them from strongest to weakest in strength
(in this
specific system).
<a
name="secretion_model"></a>Secretion Models:
Click on a specific model for more information:
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<o:p></o:p>
name="different_genes"></a>Why
test different genes in our secretion system?<o:p></o:p> There
is wide range of genes present in our environment. Therefore, it is
important
to measure our secretion system’s ability to secrete genes of various
sizes.
|
<o:p> </o:p> |
Molecular Weight<o:p></o:p> |
Number of residues<o:p></o:p> |
Number of polypeptide<o:p></o:p> |
|
Cytochrome c (human)<o:p></o:p> |
13,000<o:p></o:p> |
104<o:p></o:p> |
1<o:p></o:p> |
|
Ribonuclease A(bovine pancreas)<o:p></o:p> |
13,700<o:p></o:p> |
124<o:p></o:p> |
1<o:p></o:p> |
|
Serum albumin (human)<o:p></o:p> |
68,500<o:p></o:p> |
609<o:p></o:p> |
1<o:p></o:p> |
(Page
128, third edition “Lehninger
Principles of Biochemistry” by David L. Nelson and Michael M. Cox)<o:p>
</o:p>
<o:p></o:p>
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