Team:UC Davis/Approach

From 2009.igem.org

(Difference between revisions)
(New page: =Our Approach= We are going to break down gluten inside the stomach (before it arrives to the small intestine), because gliadin does not prompt an immune system response until it is absorb...)
(→Induced Secretion)
 
(7 intermediate revisions not shown)
Line 1: Line 1:
=Our Approach=
=Our Approach=
-
We are going to break down gluten inside the stomach (before it arrives to the small intestine), because gliadin does not prompt an immune system response until it is absorbed in the small intestine. Another worry is a spread of our synthetic bug, so we engineered a ‘safety-switch’ via induction of cell death through a pH sensor system. Due to the change of pH from the stomach to the small intestine, our pH system is induced; cell death, thus limiting our bug to the stomach.
+
Gliadin induces an immune system response when it is absorbed into the blood stream through the small intestine. We plan to take advantage of this by breaking down gliadin in the stomach before it reaches the small intestine. We have developed a secretion system to release an enzyme that can break down gliadin. Also, in order to prevent our delivery system from taking residence anywhere outside of the stomach, we plan to incorporate the difference in pH between the stomach and small intestine with a pH-inducible apoptosis system. By adopting these two systems, we will create a pH-moderated secretion system.
-
The advantages of our model are, that unlike other suggested treatments which require consuming capsules or tablets rapidly, by using ''Escherichia coli'' as our model chassis for this project: we can lower the number of times which this ‘cure’ needs to be consumed thus making it less troublesome, costly, and more convenient.
+
Unlike other suggested treatments requiring the consumption of capsules or tablets, our method of using ''E. coli'' as our delivery system is more cost- and time-efficient.
 +
 
 +
==Secretion==
 +
   
 +
*Gene sequence for secretion system
 +
*Adding Secretion
 +
*Assay for measuring protein localization and activity
 +
 
 +
==Sensing pH and Inducing Cell Death==
 +
   
 +
*Finding an appropriate biological pH sensor
 +
*Wiring the pH sensor into our system
 +
*Assay for measuring the behavior of the pH sensor
 +
 
 +
 
 +
 
 +
| [https://2009.igem.org/Team:UC_Davis Home] | [https://2009.igem.org/Team:UC_Davis/About_Us About Us] | [https://2009.igem.org/Team:UC_Davis/Celiac Celiac Disease] | [https://2009.igem.org/Team:UC_Davis/Treatments Current Treatment] | [https://2009.igem.org/Team:UC_Davis/Approach Our Approach] | [https://2009.igem.org/Team:UC_Davis/pH_Sensor pH Sensor] | [https://2009.igem.org/Team:UC_Davis/pH_Sensor/parts pH Sensor Parts] | [https://2009.igem.org/Team:UC_Davis/pH_Sensor/promoters pH Promoters] | [https://2009.igem.org/Team:UC_Davis/Secretion/parts Secretion Parts] |

Latest revision as of 22:41, 23 September 2009

Our Approach

Gliadin induces an immune system response when it is absorbed into the blood stream through the small intestine. We plan to take advantage of this by breaking down gliadin in the stomach before it reaches the small intestine. We have developed a secretion system to release an enzyme that can break down gliadin. Also, in order to prevent our delivery system from taking residence anywhere outside of the stomach, we plan to incorporate the difference in pH between the stomach and small intestine with a pH-inducible apoptosis system. By adopting these two systems, we will create a pH-moderated secretion system.

Unlike other suggested treatments requiring the consumption of capsules or tablets, our method of using E. coli as our delivery system is more cost- and time-efficient.

Secretion

  • Gene sequence for secretion system
  • Adding Secretion
  • Assay for measuring protein localization and activity

Sensing pH and Inducing Cell Death

  • Finding an appropriate biological pH sensor
  • Wiring the pH sensor into our system
  • Assay for measuring the behavior of the pH sensor


| Home | About Us | Celiac Disease | Current Treatment | Our Approach | pH Sensor | pH Sensor Parts | pH Promoters | Secretion Parts |

Retrieved from "http://2009.igem.org/Team:UC_Davis/Approach"