Team:UC Davis/Approach

From 2009.igem.org

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We are going to break down gluten inside the stomach, because gliadin does not prompt an immune system response until absorption in the small intestines.  Another worry is our delivery system taking residence somewhere outside the stomach, so we designed a pH inducible apoptosis system.  This is achieved through sensing the change of pH from the stomach to the small intestine, a difference of about four to five.
We are going to break down gluten inside the stomach, because gliadin does not prompt an immune system response until absorption in the small intestines.  Another worry is our delivery system taking residence somewhere outside the stomach, so we designed a pH inducible apoptosis system.  This is achieved through sensing the change of pH from the stomach to the small intestine, a difference of about four to five.
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The advantages of our model are that unlike other suggested treatments requiring the consumption of capsules or tablets, by using ''Escherichia coli'' as our model chassis for this project, we can lower cost and time.
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Unlike other suggested treatments requiring the consumption of capsules or tablets, our method of using ''Escherichia coli'' is more cost- and time-efficient.

Revision as of 22:19, 22 September 2009

Our Approach

We are going to break down gluten inside the stomach, because gliadin does not prompt an immune system response until absorption in the small intestines. Another worry is our delivery system taking residence somewhere outside the stomach, so we designed a pH inducible apoptosis system. This is achieved through sensing the change of pH from the stomach to the small intestine, a difference of about four to five.

Unlike other suggested treatments requiring the consumption of capsules or tablets, our method of using Escherichia coli is more cost- and time-efficient.


Induced Secretion

  • Gene sequence for secretion system
  • Adding Secretion
  • Assay for measuring protein localization and activity

Sensing pH and Inducing Cell Death

  • Finding an appropriate biological pH sensor
  • Wiring the pH sensor into our system
  • Assay for measuring the behavior of the pH sensor
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