Team:UC Davis/Notebook

From 2009.igem.org

(Difference between revisions)
Line 45: Line 45:
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <a href="#august">August</a>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <a href="#august">August</a>
&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp; <a
&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp; <a
-
href="#september">September</a><br>
+
href="#september">September</a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
<a href="#Results">Results</a><br>
<br>
<br>
<img style="width: 452px; height: 236px;" alt=""
<img style="width: 452px; height: 236px;" alt=""
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<p style="text-align: left;"><font face="Cambria" size="3"><big><big><span
<p style="text-align: left;"><font face="Cambria" size="3"><big><big><span
style="font-weight: bold;"><a name="july"></a>July:</span></big></big><br>
style="font-weight: bold;"><a name="july"></a>July:</span></big></big><br>
-
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; Before beginning the wet lab
+
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;<span style="font-family: Calibri;">
 +
Before beginning the wet lab
portion of our project, we have read numerous articles and journals
portion of our project, we have read numerous articles and journals
relevant to our proposed project, and have utilized this information to
relevant to our proposed project, and have utilized this information to
guide our part designs.&nbsp; After significant research, design and
guide our part designs.&nbsp; After significant research, design and
redesign, we have settled on the final design for our two key parts,
redesign, we have settled on the final design for our two key parts,
-
and plan for their construction.</font>&nbsp;<br>
+
and plan for their construction.</span></font><span
-
<font face="Cambria" size="3">&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; We
+
style="font-family: Calibri;">&nbsp;</span><br
 +
style="font-family: Calibri;">
 +
<font style="font-family: Calibri;" face="Cambria" size="3">&nbsp;&nbsp;&nbsp;
 +
&nbsp;&nbsp;&nbsp; We
have begun ordering the necessary reagents for our work.&nbsp; This
have begun ordering the necessary reagents for our work.&nbsp; This
includes, selecting the parts required for our project from those
includes, selecting the parts required for our project from those
Line 65: Line 70:
extracting them, and for the synthesis of novel parts via Polymerase
extracting them, and for the synthesis of novel parts via Polymerase
Chain Reaction, PCR, and the DNA synthesis of several novel parts from
Chain Reaction, PCR, and the DNA synthesis of several novel parts from
-
GENEART.</font> <font face="Cambria" size="3"><br>
+
GENEART.</font><span style="font-family: Calibri;"> </span><font
 +
style="font-family: Calibri;" face="Cambria" size="3"><br>
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; We have recently started the
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; We have recently started the
synthesis and assembly of the initial elements required for the
synthesis and assembly of the initial elements required for the
-
secretion system.</font>&nbsp;<br>
+
secretion system.</font><span style="font-family: Calibri;">&nbsp;</span><br>
</p>
</p>
<hr style="width: 100%; height: 2px;">
<hr style="width: 100%; height: 2px;">
Line 74: Line 80:
style="font-weight: bold;"><big><a name="august"></a>August:</big></big><font
style="font-weight: bold;"><big><a name="august"></a>August:</big></big><font
face="Cambria" size="3"><br>
face="Cambria" size="3"><br>
-
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; After reading the numerous
+
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; <span
 +
style="font-family: Calibri;">After reading the numerous
articles we dug up in regards to our project, we have designed our
articles we dug up in regards to our project, we have designed our
project; in blue print form of structural wet lab experimentation, as
project; in blue print form of structural wet lab experimentation, as
well as the techniques, materials, equipment, and time required to
well as the techniques, materials, equipment, and time required to
-
complete our project.</font> <font face="Cambria" size="3"><br>
+
complete our project.</span></font><span style="font-family: Calibri;">
 +
</span><font style="font-family: Calibri;" face="Cambria" size="3"><br>
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; The parts and a majority of the
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; The parts and a majority of the
reagents we have ordered necessary for our work have arrived, and we
reagents we have ordered necessary for our work have arrived, and we
Line 85: Line 93:
as well as other miscellaneous techniques that we truly have the parts
as well as other miscellaneous techniques that we truly have the parts
we want in our constructs.&nbsp; So the progress for the month of
we want in our constructs.&nbsp; So the progress for the month of
-
August varies greatly on almost a daily basis.</font>&nbsp;<br>
+
August varies greatly on almost a daily basis.</font><span
 +
style="font-family: Calibri;">&nbsp;</span><br
 +
style="font-family: Calibri;">
</div>
</div>
-
<div style="text-align: left;"><font face="Cambria" size="3">&nbsp;&nbsp;&nbsp;
+
<div style="text-align: left;"><font style="font-family: Calibri;"
 +
face="Cambria" size="3">&nbsp;&nbsp;&nbsp;
&nbsp;&nbsp;&nbsp; Apart from the wet lab portion of our project, we
&nbsp;&nbsp;&nbsp; Apart from the wet lab portion of our project, we
have also begun the initial stages of building our wiki.&nbsp; We have
have also begun the initial stages of building our wiki.&nbsp; We have
begun to revise our information regarding the team, biomedical
begun to revise our information regarding the team, biomedical
engineering, and how it is UC Davis’s inauguration year into the iGEM
engineering, and how it is UC Davis’s inauguration year into the iGEM
-
competition.</font> <br>
+
competition.</font><span style="font-family: Calibri;"> </span><br>
<hr style="width: 100%; height: 2px;"><big><big><span
<hr style="width: 100%; height: 2px;"><big><big><span
-
style="font-weight: bold;"><a name="september"></a>September:</span></big></big><br>
+
style="font-weight: bold;"><a name="september"></a>September:<br>
 +
</span></big></big>
 +
<p><font face="Times New Roman" size="3">&nbsp;&nbsp;&nbsp; <span
 +
style="font-family: Calibri;">We are putting together the final pieces
 +
of our intended construct</span></font><font
 +
style="font-family: Calibri;" face="Times New Roman" size="3"> and
 +
have designed the tiny details of our experiments’ proper controls.The
 +
design itself is useless without making it as a physical part, so we
 +
are already building these simultaneously with our experimental
 +
constructs.</font><span style="font-family: Calibri;"> </span><br
 +
style="font-family: Calibri;">
 +
<span style="font-family: Calibri;">&nbsp;&nbsp;&nbsp; </span><font
 +
style="font-family: Calibri;" face="Times New Roman" size="3">The wiki
 +
is now up on the iGEM website and is being reviewed countless times to
 +
ensure accuracy and appeal.</font><font style="font-family: Calibri;"
 +
face="Times New Roman" size="3"><br>
 +
&nbsp;&nbsp;&nbsp; We have also designed team t-shirts, which are a
 +
tradition of iGEM, and are currently in the process of making them into
 +
print.</font><span style="font-family: Calibri;"></span><font
 +
style="font-family: Calibri;" face="Times New Roman" size="3">We are
 +
also making the presentation power point for presentation at the
 +
competition.</font><span style="font-family: Calibri;"> </span><font
 +
style="font-family: Calibri;" face="Times New Roman" size="3">We are
 +
also designed the poster for presentation at the competition.</font><span
 +
style="font-family: Calibri;"><br>
 +
</span></p>
 +
<hr style="width: 100%; height: 2px;">
 +
<div style="text-align: center;"><big><big><span
 +
style="font-weight: bold;"><a name="Results"></a>Results:<br>
 +
<br>
 +
</span></big></big></div>
 +
<p><span style="font-family: Calibri;"><br>
 +
</span></p>
<br>
<br>
<br>
<br>

Revision as of 20:58, 6 October 2009

 

July                August              September          Results



July:
        Before beginning the wet lab portion of our project, we have read numerous articles and journals relevant to our proposed project, and have utilized this information to guide our part designs.  After significant research, design and redesign, we have settled on the final design for our two key parts, and plan for their construction.
 
        We have begun ordering the necessary reagents for our work.  This includes, selecting the parts required for our project from those provided by the iGEM parts distribution, oligonucleotides for extracting them, and for the synthesis of novel parts via Polymerase Chain Reaction, PCR, and the DNA synthesis of several novel parts from GENEART.
        We have recently started the synthesis and assembly of the initial elements required for the secretion system.
 


August:
        After reading the numerous articles we dug up in regards to our project, we have designed our project; in blue print form of structural wet lab experimentation, as well as the techniques, materials, equipment, and time required to complete our project.

        The parts and a majority of the reagents we have ordered necessary for our work have arrived, and we are in the stages of conducting experiments to construct our parts.  We have to construct, test, and prove through sequencing as well as other miscellaneous techniques that we truly have the parts we want in our constructs.  So the progress for the month of August varies greatly on almost a daily basis.
 
        Apart from the wet lab portion of our project, we have also begun the initial stages of building our wiki.  We have begun to revise our information regarding the team, biomedical engineering, and how it is UC Davis’s inauguration year into the iGEM competition.

September:

    We are putting together the final pieces of our intended construct and have designed the tiny details of our experiments’ proper controls.The design itself is useless without making it as a physical part, so we are already building these simultaneously with our experimental constructs.
    The wiki is now up on the iGEM website and is being reviewed countless times to ensure accuracy and appeal.
    We have also designed team t-shirts, which are a tradition of iGEM, and are currently in the process of making them into print.
We are also making the presentation power point for presentation at the competition. We are also designed the poster for presentation at the competition.


Results: