Team:UC Davis/Parts 1

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<div style="text-align: center;"><b style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></b><b
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style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><a
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href="https://2009.igem.org/Team:UC_Davis/homepage2"><img alt=""
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src="https://static.igem.org/mediawiki/2009/a/a4/UCDAVIS_PIC3.png"
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style="border: 0px solid ; width: 83px; height: 36px;"></a> </big></big></big></big></span></b><a
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href="https://2009.igem.org/Team:UC_Davis/About_Us1"><b
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style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><img
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alt="" src="https://static.igem.org/mediawiki/2009/d/d1/UCDAVIS_PIC4.png"
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style="border: 0px solid ; width: 82px; height: 36px;"></big></big></big></big></span></b></a><b
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style="color: rgb(255, 255, 153);"><span
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style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big>
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</big></big></big></big></span></b><b style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><a
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href="https://2009.igem.org/Team:UC_Davis/Project1"><img alt=""
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src="https://static.igem.org/mediawiki/2009/b/b9/UCDAVIS_PIC8.png"
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style="border: 0px solid ; width: 78px; height: 36px;"></a> </big></big></big></big></span></b><b
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style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><a
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href="https://2009.igem.org/Team:UC_Davis/Notebook1"><img alt=""
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src="https://static.igem.org/mediawiki/2009/2/2f/UCDAVIS_PIC5.png"
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style="border: 0px solid ; width: 81px; height: 36px;"></a> </big></big></big></big></span></b><b
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style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></b><b
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style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><a
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href="https://2009.igem.org/Team:UC_Davis/Part1"><img alt=""
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src="https://static.igem.org/mediawiki/2009/a/a6/UCDAVIS_PIC6.png"
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style="border: 0px solid ; width: 78px; height: 37px;"></a>&nbsp;<a
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href="https://2009.igem.org/Team:UC_Davis/Contact1"><img alt=""
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src="https://static.igem.org/mediawiki/2009/1/1d/UCDAVIS_PIC7.png"
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style="border: 0px solid ; width: 83px; height: 37px;"></a></big></big></big></big></span></b><b
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style="color: rgb(0, 0, 0);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><small>
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</small></big></big></big></big></span></b>
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<hr style="width: 100%; height: 2px;">
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<div style="text-align: left;"><big><big><b
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style="color: rgb(0, 0, 0); text-decoration: underline;">Parts:</b></big></big><b
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style="color: rgb(0, 0, 0);">&nbsp;&nbsp; <br>
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<br>
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Parts related to
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secretion:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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Parts related to pH sensor:<br>
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</b>
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<table
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style="text-align: left; width: 100%; background-color: rgb(255, 255, 0);"
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border="2" cellpadding="2" cellspacing="2">
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<tbody>
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<tr>
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<td style="vertical-align: top;">Proteins:<br>
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</td>
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<td style="vertical-align: top;">Promoters:<br>
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</td>
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<td style="vertical-align: top;">Others:<br>
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</td>
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<td style="vertical-align: top;">Proteins:<br>
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</td>
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<td style="vertical-align: top;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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Promoters:<br>
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</td>
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</tr>
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<tr>
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<td style="vertical-align: top;">
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<ul>
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<li><a href="#INPNC">INPNC</a></li>
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<li><a href="#OmpA">OmpA</a></li>
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<li><a href="#Luciferase">Luciferase</a></li>
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<li><a href="#GFP">GFP</a></li>
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</ul>
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</td>
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<td style="vertical-align: top;">
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<ul>
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<li><a href="#LacI">LacI</a></li>
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</ul>
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</td>
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<td style="vertical-align: top;">
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<ul>
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<li><a href="#SS">SS:signal sequence</a></li>
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<li><a href="#His">6-His-Tag</a></li>
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<li><a href="#RBS">RBS</a></li>
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<li><a href="#Terminator">Terminator</a></li>
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</ul>
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</td>
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<td style="vertical-align: top;">
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<ul>
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<li>ChvI</li>
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<li>ChvG</li>
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</ul>
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</td>
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<td style="vertical-align: top;">
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<ul>
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<li><a href="#ChvI_promoter">ChvI promoter</a></li>
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<li><a href="#katA">KatA promoter</a></li>
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<li><a href="#aopB">AopB promoter</a></li>
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<li><a href="#PhoA">PhoA
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promoter</a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp;&nbsp; </li>
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<li>I<a href="#impA">mpA promoter</a></li>
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</ul>
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</td>
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</tr>
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</tbody>
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</table>
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<b style="color: rgb(0, 0, 0);">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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</b><br>
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<hr style="width: 100%; height: 2px;">
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<p class="MsoNormal" style=""><b><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><a
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name="INPNC"></a>INPNC</span></b><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">:
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Ice-nucleation protein (INP) from Pseudomonas Syringae was
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suggested to be used for display of foreign proteins on the surface of <i>E.coli</i>(7).Furthermore,
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researches have shown that an INP derivative constituting the N-and
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C-terminal
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domains can and has been used to display foreign proteins on the
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surface of <i>E.coli</i>(9).
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</span><span style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
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<p class="MsoNormal" style=""><i><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">Note:
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A study has shown that "Ice- nucleation Protein (INP), an outer
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membrane
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protein from Pseudomonas syringae, is able to catalyze the ice crystal
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formation of supercooled water.</span></i><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">In
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our project we are intending to harness and make use of this feature by
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fusing
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a specific protein to it. <i>We have modified this protein to Biobrick
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standard, Tom Knights Standard.</i></span><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
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<div class="MsoNormal" style="text-align: center;" align="center"><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">
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<hr align="center" size="2" width="100%"></span></div>
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<p class="MsoNormal" style=""><a name="OmpA"></a><b><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">OmpA</span></b><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">:
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OmpA is one of the proteins on the outer membrane of <i>E.coli</i>
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(13). OmpA has been found to be useful as utilizable fusion part that
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can fuse
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our protein to and display on the surface of <i>E.coli</i>. This part
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has
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already been documented on the parts registry; however, it has not been
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tested
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via fusion with a target protein linked with a cleavable signal
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sequence.<u1:p></u1:p></span><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><br>
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</span><i><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">We
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have modified this protein to Biobrick standard, Tom Knights
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Standard. </span></i><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><u1:p></u1:p><o:p></o:p></span></p>
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<p class="MsoNormal" style=""><i><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">Note:</span></i><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">
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“<i>It has
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remained essentially unknown how proteins of Escherichia coli outer
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membrane
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are sorted and incorporated into this membrane” (10)<br>
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<u1:p></u1:p>For more information go to:</i> <a
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href="http://partsregistry.org/wiki/index.php?title=Part:BBa_J36836"><i><span
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style="">http://partsregistry.org/wiki/index.php?title=Part:BBa_J36836</span></i></a></span><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
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<div class="MsoNormal" style="text-align: center;" align="center"><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">
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<hr align="center" size="2" width="100%"></span></div>
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<p class="MsoNormal" style=""><a name="RBS"></a><b><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><u1:p></u1:p>RBS</span></b><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">:
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Ribosome Binding site number 32 (BBa_J61132) from the registry is
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being used in our secretion system.<u1:p></u1:p></span><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><br>
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</span><i><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">For
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more information go to:</span></i><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">
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<a href="http://partsregistry.org/wiki/index.php/Part:BBa_J61132"><i><span
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style="">http://partsregistry.org/wiki/index.php/Part:BBa_J61132</span></i></a></span><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
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<div class="MsoNormal" style="text-align: center;" align="center"><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">
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<hr align="center" size="2" width="100%"></span></div>
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<p class="MsoNormal" style=""><a name="Terminator"></a><b><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><u1:p></u1:p>Terminator</span></b><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">:
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We are using BBa_B0015, a double terminator, as our terminator in
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both our secretion and pH system.<u1:p></u1:p></span><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><br>
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</span><i><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">For
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more information go to:</span></i><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015"><i><span
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style="">http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015</span></i></a></span><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
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<div class="MsoNormal" style="text-align: center;" align="center"><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">
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<hr align="center" size="2" width="100%"></span></div>
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<p class="MsoNormal" style=""><a name="GFP"></a><b><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><u1:p></u1:p>GFP</span></b><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">:
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We are using Green Fluorescent Protein as a reporter that also
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serves as a small protein in testing our secretion system.</span><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
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<div class="MsoNormal" style="text-align: center;" align="center"><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">
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<hr align="center" size="2" width="100%"></span></div>
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<p class="MsoNormal" style=""><a name="Luciferase"></a><b><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><u1:p></u1:p>Luciferase</span></b><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">:
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Luciferase is a firefly protein that also fluoresces, so it
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serves as a reporter as well as a testable large protein.<i><br>
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More can be found in:</i></span><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
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<div class="MsoNormal" style="text-align: center;" align="center"><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">
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<hr align="center" size="2" width="100%"></span></div>
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<p class="MsoNormal" style=""><a name="LacI"></a><b><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">LacI</span></b><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">:
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One inducible Promoter which was found in the part registry.<i><br>
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More can be found in: </i><a
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href="http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010"><i><span
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style="">http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010</span></i></a></span><span
+
-
style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
+
-
<div class="MsoNormal" style="text-align: center;" align="center"><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">
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<hr align="center" size="2" width="100%"></span></div>
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<p class="MsoNormal"><a name="SS"></a><b><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">SS</span></b><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">:
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This signal sequence, when placed between INPNC, contains a
+
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cleavable site that allows the target fusion protein to ‘secrete’ from
+
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INPNC.&nbsp; We will do the same with OmpA.&nbsp; <u1:p></u1:p></span><span
+
-
style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
+
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<p class="MsoNormal" style=""><i><span
+
-
style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">We
+
-
have modified this protein to Biobrick standard, Tom Knights
+
-
Standard.</span></i><span
+
-
style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
+
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<div class="MsoNormal" style="text-align: center;" align="center"><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">
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<hr align="center" size="2" width="100%"></span></div>
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<p class="MsoNormal" style=""><a name="His"></a><b><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">6-His
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Tag</span></b><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">:
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The 6-Histidine Tag serves as a tag for Western Blotting if our
+
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fluorescent reporters are not expressed as highly as we would like.<u1:p></u1:p></span><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><br>
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</span><i><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">Note:
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We are using this tag, just in case if the GFP or Luciferase
+
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does not work under a plate reader.</span></i><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
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<div class="MsoNormal" style="text-align: center;" align="center"><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">
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<hr align="center" size="2" width="100%"></span></div>
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<p class="MsoNormal" style=""><a name="ChvI_promoter"></a><b><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">ChvI
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promoter</span></b><span
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-
style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">:
+
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Gene fusion studies confirmed that ChvI gene was induced by
+
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acidic conditions (1). Also, it has been known to implicate in
+
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virulence (1).
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This gene is one of the candidates to be use in our biological pH
+
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sensor as a
+
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promoter.</span><span style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
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<div class="MsoNormal" style="text-align: center;" align="center"><span
+
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">
+
-
<hr align="center" size="2" width="100%"></span></div>
+
-
<p class="MsoNormal" style=""><b><span
+
-
style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><a
+
-
name="katA"></a>KatA promoter</span></b><span
+
-
style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">
+
-
:This
+
-
Chromosomal gene is located on the linear chromosome (2) and it seems
+
-
to be
+
-
induced under an acidic environment as well as being involved in the <i>Agrobacterium
+
-
tumorigenesis</i> (2).Research has suggested that ChvG is needed for
+
-
"responsiveness of&nbsp; gene expression to low pH "(2). This gene
+
-
has become a candidate to complete our pH sensor device from this
+
-
evidence.</span><span style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
+
-
<div class="MsoNormal" style="text-align: center;" align="center"><span
+
-
style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">
+
-
<hr align="center" size="2" width="100%"></span></div>
+
-
<p class="MsoNormal" style=""><a name="aopB"></a><b><span
+
-
style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">AopB
+
-
promoter</span></b><span
+
-
style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">:
+
-
This Chromosomal gene located on the circular chromosome (2)
+
-
encodes an outer member protein exposed on the bacterial cell surface
+
-
(2).
+
-
Also, ChvG was shown to be absolutely required for this gene expression
+
-
(2)It
+
-
seems to get induced under an acidic environment as well as being
+
-
involved in
+
-
the <i>Agrobacterium</i> <i>tumorigenesis </i>(2). Therefore, we
+
-
have chosen
+
-
this gene to be one of our candidates to complete our pH sensor device.</span><span
+
-
style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
+
-
<div class="MsoNormal" style="text-align: center;" align="center"><span
+
-
style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">
+
-
<hr align="center" size="2" width="100%"></span></div>
+
-
<p class="MsoNormal" style=""><a name="PhoA"></a><b><span
+
-
style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><u1:p></u1:p>PhoA
+
-
promoter</span></b><span
+
-
style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">:
+
-
There has been a suggestion that ChvI can activate AP activity by
+
-
activating transcription of this gene, PhoA (3). Therefore, this gene
+
-
has
+
-
become one of our candidates to complete our pH sensor device.</span><span
+
-
style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
+
-
<div class="MsoNormal" style="text-align: center;" align="center"><span
+
-
style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">
+
-
<hr align="center" size="2" width="100%"></span></div>
+
-
<p class="MsoNormal"><a name="impA"></a><b><span
+
-
style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><u1:p></u1:p>ImpA
+
-
promoter: </span></b><span
+
-
style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">Gene
+
-
fusion studies confirmed that
+
-
impA genes was induced by acidic conditions (1), therefore, this is one
+
-
of our
+
-
candidates to complete our pH sensor device.</span><b><u><span
+
-
style="font-size: 12pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></u></b></p>
+
-
<p class="MsoNormal"><span
+
-
style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p>&nbsp;</o:p></span></p>
+
-
<hr style="width: 100%; height: 2px;"><small style="font-weight: bold;"><span
+
-
style="font-size: 18pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></small></div>
+
-
</div>
+
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</body>
+
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</html>
+

Latest revision as of 21:40, 25 September 2009

Retrieved from "http://2009.igem.org/Team:UC_Davis/Parts_1"