Team:UC Davis/PhoR ChvI1

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<br>
<br>
</span></big></big>&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;
</span></big></big>&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;
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&nbsp;&nbsp;&nbsp; It has been hypothesized
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&nbsp;&nbsp;&nbsp; </span></b><span
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style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">It
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has been hypothesized
that due to the amino acid similarity of ChvI to PhoB, ChvI is able to
that due to the amino acid similarity of ChvI to PhoB, ChvI is able to
act as a
act as a
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phosphate regulatory in <i>E.coli</i>, as a PhoB substitute (3).</span></b><span
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phosphate regulatory in <i>E.coli</i>, as a PhoB substitute (3).</span><span
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<span style="">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>Since
<span style="">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>Since
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limitation, it
limitation, it
cannot serve as the primary phosphate regulator in <i>Agrobacterium</i>
cannot serve as the primary phosphate regulator in <i>Agrobacterium</i>
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(3).<o:p></o:p></span></b></p>
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(3).<o:p></o:p></span></p>
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in Agrobacterium Tumefaciens (3), but it might be involved in phosphate
in Agrobacterium Tumefaciens (3), but it might be involved in phosphate
regulation in Agrobacterium Tumefaciens in some way (3). Therefore, the
regulation in Agrobacterium Tumefaciens in some way (3). Therefore, the
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possibility of crosstalk still exists</i><o:p></o:p></span></b></p>
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possibility of crosstalk still exists</i><o:p></o:p></span></p>
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style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">&nbsp;&nbsp;<span
style="">&nbsp;&nbsp; </span>&nbsp;Due to the structural similarity
style="">&nbsp;&nbsp; </span>&nbsp;Due to the structural similarity
of
of
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ChvI and PhoB, the possibility of crosstalk exists (3).<o:p></o:p></span></b></p>
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ChvI and PhoB, the possibility of crosstalk exists (3).<o:p></o:p></span></p>
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<p class="MsoNormal"><b><i><span
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<p class="MsoNormal"><i><span
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Note: Since
Note: Since
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had to make sure that our proteins will keep their characteristics of
had to make sure that our proteins will keep their characteristics of
our
our
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interest after being placed inside the Escherichia coli.</span></i></b><b><span
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interest after being placed inside the Escherichia coli.</span></i><span
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<i>For example, VirG gene (promoter 1) after being introduced
<i>For example, VirG gene (promoter 1) after being introduced
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was due to the fact that virG promoter 1 has two hexamer blocks which
was due to the fact that virG promoter 1 has two hexamer blocks which
have the
have the
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same positions as the pho genes (6).</i> <o:p></o:p></span></b></p>
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same positions as the pho genes (6).</i> <o:p></o:p></span></p>
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</span>Studies have shown that ChvI probably activated Alkaline
</span>Studies have shown that ChvI probably activated Alkaline
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Phosphatase</span></b><em><span
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Phosphatase</span><em><span
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style="font-size: 13pt;">(AP)</span></b></em><b><span
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style="font-size: 13pt;">(AP)</span></em><span
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activity by activating transcription of PhoA (PhoA also gets activated
activity by activating transcription of PhoA (PhoA also gets activated
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that since PhoR mediates PhoB phosphorylation and dephosphorylation, it
that since PhoR mediates PhoB phosphorylation and dephosphorylation, it
may do
may do
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the same to ChvI(3).<o:p></o:p></span></b></p>
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the same to ChvI(3).<o:p></o:p></span></p>
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Therefore, the best option would be to find a promoter
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</span>Therefore, the best option would be to find a promoter
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from <i>Agrobacterium</i> that gets activated by ChvG/ChvI, a
from <i>Agrobacterium</i> that gets activated by ChvG/ChvI, a
two-component
two-component
regulatory system only; and does not interact with any <i>E. coli</i>
regulatory system only; and does not interact with any <i>E. coli</i>
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system.<br>
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system.</span><b><span
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</span></b></p>
</span></b></p>
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Revision as of 20:12, 25 September 2009

PhoR to ChvI

 

PhoR-->ChvI

            It has been hypothesized that due to the amino acid similarity of ChvI to PhoB, ChvI is able to act as a phosphate regulatory in E.coli, as a PhoB substitute (3).

            Since ChvI showed no abnormal activity inside Agrobacterium Tumefaciens under phosphate limitation, it cannot serve as the primary phosphate regulator in Agrobacterium (3).

            Note: ChvI was not required for phosphate regulation in Agrobacterium Tumefaciens (3), but it might be involved in phosphate regulation in Agrobacterium Tumefaciens in some way (3). Therefore, the possibility of crosstalk still exists

      Due to the structural similarity of ChvI and PhoB, the possibility of crosstalk exists (3).

    Note: Since we are going to use Escherichia coli as our model chassis for this project; we had to make sure that our proteins will keep their characteristics of our interest after being placed inside the Escherichia coli. For example, VirG gene (promoter 1) after being introduced into E.coli cells, started to behave as if it were a member of phosphate regulon (note: PhoB function was necessary for virG induction by phosphate starvation)(6). Studies have suggested that the reason for this behavior was due to the fact that virG promoter 1 has two hexamer blocks which have the same positions as the pho genes (6).

            Studies have shown that ChvI probably activated Alkaline Phosphatase (AP) activity by activating transcription of PhoA (PhoA also gets activated by PhoB) (3). It was not proven that ChvI activates other PhoB-dependent promoters or recognizes the same DNA sequence as PhoB (3).But, there has been speculation that since PhoR mediates PhoB phosphorylation and dephosphorylation, it may do the same to ChvI(3).

            Therefore, the best option would be to find a promoter from Agrobacterium that gets activated by ChvG/ChvI, a two-component regulatory system only; and does not interact with any E. coli system.

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