Team:UC Davis/Project1/Finding ph sensor

From 2009.igem.org

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Revision as of 21:02, 15 October 2009

Finding an appropriate biological pH sensor:

Why is there a need for a pH sensor?

We would like our probiotic organism to confine itself to the stomach. Since low pH is a hallmark of the stomach, it seems reasonable to assume that linking the production of a suicide gene to a dramatic increase in pH (to values found in the intestine) should allow us to sequester our organism to the stomach. There are no pH sensors available on the registry of biological parts. So, we will engineer a device to sense extracellular pH changes and design it so that it can be used to regulate the expression of any target gene in confinement by pH levels, and then add it to the registry!

Finding an appropriate biological pH sensor:

We have decided to design our pH sensor by adopting one of the pH sensors of Agrobacterium Tumefaciens. The ChvG/ChvI two component system of Agrobacterium is suspected to sense extracellular pH changes (2) and relay this environmental signal to numerous virulence related promoters (2).

For more information regarding ChvG/ChvI two component system, go to ChvG, ChvI, ChvG--->ChvI

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-href="https://2009.igem.org/Team:UC_Davis/Contacts_References"><img alt=""
+href="https://2009.igem.org/Team:UC_Davis/Contacts_References"><img
-src="https://static.igem.org/mediawiki/2009/1/1d/UCDAVIS_PIC7.png"
+alt="" src="https://static.igem.org/mediawiki/2009/1/1d/UCDAVIS_PIC7.png"
 style="border: 0px solid ; width: 83px; height: 37px;"></a></big></big></big></big></span></b><b
 style="color: rgb(0, 0, 0);"><span
-style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><small></small></big></big></big></big></span><br>
+style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span><br>
 </b>
 <hr style="width: 100%; height: 2px;">
 <p class="MsoNormal"
-style="background: white none repeat scroll 0%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left; text-decoration: underline;"><big><big><b><span
+style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left; text-decoration: underline;"><big><big><b><span
 style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">Finding
 an appropriate biological pH sensor:</span></b></big></big></p>
 <p class="MsoNormal"
-style="background: white none repeat scroll 0%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><b><span
+style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><b><span
 style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">Why
 is there a need for a pH sensor?<o:p></o:p></span></b></p>
-<div style="text-align: left;"></div>
 <p class="MsoNormal"
-style="background: white none repeat scroll 0%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><span
+style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><span
 style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We
 would like our probiotic organism to confine itself to the stomach.
@@ Line 69: / Line 68: @@
 confinement by pH
 levels, and then add it to the registry!<o:p></o:p></span></p>
-<div style="text-align: left;"></div>
 <p class="MsoNormal"
-style="background: white none repeat scroll 0%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><b><span
+style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><b><span
 style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">Finding
 an appropriate biological pH sensor:</span></b><b><span
 style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></b></p>
-<div style="text-align: left;"></div>
 <p class="MsoNormal"
-style="background: white none repeat scroll 0%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><span
+style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><span
 style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 We have decided to design our pH sensor by adopting one of the pH
@@ Line 86: / Line 83: @@
 environmental
 signal to numerous virulence related promoters (2). <o:p></o:p></span></p>
-<div style="text-align: left;"></div>
 <p class="MsoNormal"
-style="background: white none repeat scroll 0%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><span
+style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><span
 style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
-<i>For more information regarding ChvG, go to </i></span><span
+<i>For more information regarding </i></span><span
-style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><a
+style="font-style: italic;">ChvG/ChvI two component system</span><span
-href="ChvG.html"><i><span style="color: blue;">ChvG</span></i></a><o:p></o:p></span></p>
+style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;"><i>,
-<div style="text-align: left;"></div>
+go to <a href="https://2009.igem.org/Team:UC_Davis/Project1/ChvG.html">ChvG</a>,
+<a href="https://2009.igem.org/Team:UC_Davis/ChvI1">ChvI</a>, <a
+href="https://2009.igem.org/Team:UC_Davis/ChvG_ChvI">ChvG---&gt;ChvI</a><br>
+</i></span></p>
+<hr style="width: 100%; height: 2px;">
 <p class="MsoNormal"
-style="background: white none repeat scroll 0%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><span
+style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><br>
-style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">Once
+<span
-our pH sensor is built, we will need to test its behavior in
+style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;"></span><span
-response to changes to external pH.<o:p></o:p></span></p>
+style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><a
-<div style="text-align: left;"></div>
+href="https://2009.igem.org/Team:UC_Davis/ChvG_ChvI"><i><span
+style="color: blue;"></span></i></a><o:p></o:p></span></p>
 <p class="MsoNormal"
-style="background: white none repeat scroll 0%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><span
+style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><i><u><span
-style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<i>For
+style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: blue;"></span></u></i><span
-more information go to </i></span><i><u><span
-style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: blue;">assay
-for ph sensor</span></u></i><span
 style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;"><o:p></o:p></span></p>
 </div>
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