Team:UC Davis/assay for measuring protein localization and activity
From 2009.igem.org
(Difference between revisions)
(New page: <html> <head> <title></title> </head> <body> <div style="text-align: center;"><b style="color: rgb(255, 255, 153);"><span style="font-size: 13.5pt; font-family: "Times New Roman"...) |
|||
Line 48: | Line 48: | ||
<div style="text-align: left;"><br> | <div style="text-align: left;"><br> | ||
We will utilize the Western | We will utilize the Western | ||
- | blotting technique to identify and quantify our proteins | + | blotting technique to identify and quantify our proteins, GFP and Luciferase, and also use a plate reader to measure the amount of fluorescence. |
- | use a plate reader to | + | <br> |
- | + | ||
<hr style="width: 100%; height: 2px;"><br> | <hr style="width: 100%; height: 2px;"><br> | ||
</div> | </div> |
Revision as of 07:55, 24 September 2009
Assay for
measuring protein localization and activity:
We will utilize the Western blotting technique to identify and quantify our proteins, GFP and Luciferase, and also use a plate reader to measure the amount of fluorescence.
We will utilize the Western blotting technique to identify and quantify our proteins, GFP and Luciferase, and also use a plate reader to measure the amount of fluorescence.