Team:UC Davis/wiring pH sensor

From 2009.igem.org

(Difference between revisions)
 
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Th pH sensor: </span></big><br>
Th pH sensor: </span></big><br>
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; ChvG/ChvI is a
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; ChvG/ChvI is a
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two-component system and a suspected pH sensor. ChvG is the histidine kinase and ChvI is its
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two-component system and a suspected pH sensor. ChvG is the histidine
 +
kinase and ChvI is its
cognate response regulator(2). Together they are believed to sense
cognate response regulator(2). Together they are believed to sense
extracellular pH between 5.5 to 7 and relay this signal to regulate
extracellular pH between 5.5 to 7 and relay this signal to regulate
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Cross-Talk:</span></big><br>
Cross-Talk:</span></big><br>
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; Bacteria have
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; Bacteria have
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numerous related two-component systems based on kinase/response-regulation(5). For instance, the <i>A. tumefaciens</i>
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numerous related two-component systems based on
 +
kinase/response-regulation(5). For instance, the <i>A. tumefaciens</i>
genome is known to contain at least 25 two-component pathways
genome is known to contain at least 25 two-component pathways
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(2). Therefore, there are possibilities of cross-talk between some genes
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(2). Therefore, there are possibilities of cross-talk between some
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present in <i>E. coli</i> that have the same mechanism as the ChvI/ChvG
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genes
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present in <i>E. coli</i> that have the same mechanism as the
 +
ChvI/ChvG
system. In fact, there is evidence supporting that ChvI can complement
system. In fact, there is evidence supporting that ChvI can complement
a PhoB deletion in <i>E. coli</i>. (3) Therefore it is highly likely
a PhoB deletion in <i>E. coli</i>. (3) Therefore it is highly likely
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<br>
<br>
<hr style="width: 100%; height: 2px;">
<hr style="width: 100%; height: 2px;">
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<div style="text-align: center;"><span style="font-style: italic;"></span><br>
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<div style="text-align: center;"><span style="font-style: italic;"></span><img
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style="width: 809px; height: 464px;" alt=""
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src="https://static.igem.org/mediawiki/2009/e/ea/UCDAVIS_pH_sensor1.jpg"><br>
<span style="font-style: italic;"></span></div>
<span style="font-style: italic;"></span></div>
<span style="font-style: italic;">Click on the specific part or
<span style="font-style: italic;">Click on the specific part or

Latest revision as of 02:41, 22 October 2009

 

Wiring the pH sensor into our system:

Th pH sensor:

            ChvG/ChvI is a two-component system and a suspected pH sensor. ChvG is the histidine kinase and ChvI is its cognate response regulator(2). Together they are believed to sense extracellular pH between 5.5 to 7 and relay this signal to regulate the expression of key virulence-related genes(2).
In our design, we used this two-component system from Agrobacterium tumefaciens to regulate the expression of select target genes.

Possibility of Cross-Talk:
            Bacteria have numerous related two-component systems based on kinase/response-regulation(5). For instance, the A. tumefaciens genome is known to contain at least 25 two-component pathways (2). Therefore, there are possibilities of cross-talk between some genes present in E. coli that have the same mechanism as the ChvI/ChvG system. In fact, there is evidence supporting that ChvI can complement a PhoB deletion in E. coli. (3) Therefore it is highly likely that there is a possibility of cross-talk between our new sensory systems elements, present in E. coli. Many protein-protein interactions are not fully understood (5), which makes this project more challenging but very interesting.


Click on the specific part or interaction for more information.
Parts: ChvG, ChvI, PhoR, PhoB
Interactions: ChvG---> ChvI , PhoR---> ChvI , PhoR--->PhoB ,ChvI--->PhoA