Team:ULB-Brussels/Parts

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<li><a class="mainlink" href="/Team:ULB-Brussels/Project">Project</a></li>
<li><a class="mainlink" href="/Team:ULB-Brussels/Project">Project</a></li>
<li><a class="mainlink selection" href="/Team:ULB-Brussels/Parts">Parts</a></li>
<li><a class="mainlink selection" href="/Team:ULB-Brussels/Parts">Parts</a></li>
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<li><a class="mainlink" href="/Team:ULB-Brussels/Modeling">Modeling</a></li>
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<li><a class="mainlink" href="/Team:ULB-Brussels/Safety">Safety</a></li>
<li><a class="mainlink" href="/Team:ULB-Brussels/Notebook">Notebook</a></li>
<li><a class="mainlink" href="/Team:ULB-Brussels/Notebook">Notebook</a></li>
<li><a class="mainlink" href="/Team:ULB-Brussels/Sponsors">Sponsors</a></li>
<li><a class="mainlink" href="/Team:ULB-Brussels/Sponsors">Sponsors</a></li>
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=CcdA antidote with the mob promoter=
 
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<p>
 
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Stabilization system : Higher plasmid stability = More proteins Principle: In the StabyExpressTM system, the antidote gene (ccdA) is introduced in the plasmid DNA under the control of a weak constitutive promoter : the mob promoter, which doesn’t come from E. coli but from a broad host range plasmid (pBHR1) . On the other hand, the toxic gene (ccdB) is introduced in the chromosome of the bacteria, which can be furnished by DelphiGenetics. Expression of the poison gene is under the control of a promoter strongly repressed in the presence of the plasmid. When the plasmid is lost, the antidote is degraded and the production of the toxin is induced, causing cell death. Practically this means that when during the pre-induction phase bacteria are grown, 100% of the bacteria will carry the vector. If they lose the vector, they will not obtain a growth advantage, but will die. Upon induction 100% of the bacteria will start producing the recombinant protein leading to higher yields of the target protein and less background caused by unwanted proteins. For manufacturers of recombinant proteins this system offers a great benefit because it is an antibiotic free expression system. Therefore the manufactured protein will also be free of traces of antibiotics. </p>
 
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For more information, please visit the [http://www.delphigenetics.com Delphi Genetics's site]
 
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{| class="wikitable " style="text-align:center; width:80%;"cellpadding="0" cellspacing="0" border="1"
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|+
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|- style="background:#5F91C1;  text-align: center"
 +
!scope=col | NAME
 +
!scope=col | FEATURE
 +
!scope=col | SEQUENCE
 +
!scope=col |LENGTH (bp)
 +
!scope=col | DNA SUBMITTED
 +
!scope=col | DESCRIPTION
 +
 +
|- style="text-align: center"
 +
 +
|<partinfo>BBa_K196000</partinfo>
 +
|Other
 +
|<partinfo>K196000 DeepComponents </partinfo>
 +
|481
 +
|Yes
 +
|CcdA antidote with the mob promoter (reverse)
 +
 +
|-style="text-align: center"
 +
|<partinfo>BBa_K196001</partinfo>
 +
|Other
 +
|<partinfo>K196001 DeepComponents </partinfo>
 +
|481
 +
|Yes
 +
|CcdA antidote with the mob promoter (forward)
 +
 +
|-style="text-align: center"
 +
|<partinfo>BBa_K196002</partinfo>
 +
|Coding
 +
|<partinfo>K196002 DeepComponents </partinfo>
 +
|933
 +
|Yes
 +
|HfsG protein from Caulobacter crescentus
 +
 +
|-style="text-align: center"
 +
|<partinfo>BBa_K196003</partinfo>
 +
|Coding
 +
|<partinfo>K196003 DeepComponents </partinfo>
 +
|777
 +
|Yes
 +
|HfsH protein from Caulobacter crescentus
 +
 +
|-style="text-align: center"
 +
|<partinfo>BBa_K196004</partinfo>
 +
|Coding
 +
|<partinfo>K196004 DeepComponents </partinfo>
 +
|1716
 +
|
 +
|HfsG + HfsH proteins from Caulobacter crescentus
 +
 +
|-style="text-align: center"
 +
|<partinfo>BBa_K196005</partinfo>
 +
|Intermediate
 +
|<partinfo>K196005 DeepComponents </partinfo>
 +
|1853
 +
|Yes
 +
|HfsG + HfsH proteins from Caulobacter crescentus + ter (BBa_B0015)
 +
 +
|-style="text-align: center"
 +
|<partinfo>BBa_K196006</partinfo>
 +
|Intermediate
 +
|<partinfo>K196006 SpecifiedComponents</partinfo>
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|75
 +
|
 +
|Promoter (lacI regulated, lambda pL hybrid) + RBS (Elowitz 1999)
 +
 +
|-style="text-align: center"
 +
|<partinfo>BBa_K196007</partinfo>
 +
|Intermediate
 +
|<partinfo>K196007 DeepComponents </partinfo>
 +
|787
 +
|Yes
 +
|Promoter (lacI regulated, lambda pL hybrid) + RFP with RBS
 +
 +
|-style="text-align: center"
 +
|<partinfo>BBa_K196008</partinfo>
 +
|Generator
 +
|<partinfo>K196008 DeepComponents </partinfo>
 +
|884
 +
|
 +
|Lux cassette + LacI promoter + cI434 + ter
 +
 +
|-style="text-align: center"
 +
|<partinfo>BBa_K196009</partinfo>
 +
|Generator
 +
|<partinfo>K196009 DeepComponents </partinfo>
 +
|886
 +
|
 +
|CI434 promoter + RBS + c2 P22 + ter
 +
 +
|-style="text-align: center"
 +
|<partinfo>BBa_K196010</partinfo>
 +
|Signalling
 +
|<partinfo>K196010 DeepComponents</partinfo>
 +
|838
 +
|
 +
|Lux cassette + c2 P22 promoter + RBS + LuxI + ter
 +
 +
|-style="text-align: center"
 +
|<partinfo>BBa_K196011</partinfo>
 +
|Signalling
 +
|<partinfo>K196011 DeepComponents</partinfo>
 +
|998
 +
|
 +
|c2 P22 promoter + RBS + LuxR + ter
-
==CcdA antidote with the mob promoter (reversed)==
+
|-style="text-align: center"
 +
|<partinfo>BBa_K196012</partinfo>
 +
|Device
 +
|<partinfo>K196012 DeepComponents</partinfo>
 +
|2252
 +
|
 +
|Glue synthesizer(inhibited replication)
-
<partinfo>BBa_K196000</partinfo>
+
|-style="text-align: center"
 +
|<partinfo>BBa_K196013</partinfo>
 +
|Signaling
 +
|<partinfo>K196013 DeepComponents</partinfo>
 +
|976
 +
|
 +
|Hybrid promoter (Lux cassette + c2 P22 promoter) + RBS + LuxR + ter
 +
|-style="text-align: center"
 +
|<partinfo>BBa_K196014</partinfo>
 +
|Generator
 +
|<partinfo>K196014 DeepComponents</partinfo>
 +
|2646
 +
|Yes
 +
|IPTG-induced glue synthetiser
-
==CcdA antidote with the mob promoter (forward)==
+
|}
-
<partinfo>BBa_K196001</partinfo>
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Latest revision as of 21:19, 21 October 2009

iGEM Team:ULB-Brussels Wiki

NAME FEATURE SEQUENCE LENGTH (bp) DNA SUBMITTED DESCRIPTION
Other 481 Yes CcdA antidote with the mob promoter (reverse)
Other 481 Yes CcdA antidote with the mob promoter (forward)
Coding 933 Yes HfsG protein from Caulobacter crescentus
Coding 777 Yes HfsH protein from Caulobacter crescentus
Coding 1716 HfsG + HfsH proteins from Caulobacter crescentus
Intermediate 1853 Yes HfsG + HfsH proteins from Caulobacter crescentus + ter (BBa_B0015)
Intermediate 75 Promoter (lacI regulated, lambda pL hybrid) + RBS (Elowitz 1999)
Intermediate 787 Yes Promoter (lacI regulated, lambda pL hybrid) + RFP with RBS
Generator 884 Lux cassette + LacI promoter + cI434 + ter
Generator 886 CI434 promoter + RBS + c2 P22 + ter
Signalling 838 Lux cassette + c2 P22 promoter + RBS + LuxI + ter
Signalling 998 c2 P22 promoter + RBS + LuxR + ter
Device 2252 Glue synthesizer(inhibited replication)
Signaling 976 Hybrid promoter (Lux cassette + c2 P22 promoter) + RBS + LuxR + ter
Generator 2646 Yes IPTG-induced glue synthetiser
Retrieved from "http://2009.igem.org/Team:ULB-Brussels/Parts"