http://2009.igem.org/wiki/index.php?title=Team:UNICAMP-Brazil/Coliguard/Results&feed=atom&action=historyTeam:UNICAMP-Brazil/Coliguard/Results - Revision history2024-03-28T20:59:31ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:UNICAMP-Brazil/Coliguard/Results&diff=169075&oldid=prevFabi at 03:56, 22 October 20092009-10-22T03:56:07Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:UNICAMP-Brazil/inc_topo}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:UNICAMP-Brazil/inc_topo}}</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">=='''The Coliguard - Results'''==</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>===<del class="diffchange diffchange-inline">The Coliguard - Results</del>=<del class="diffchange diffchange-inline">==</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==== Test and characterization of device''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284022 BBa_K284022] <ins class="diffchange diffchange-inline">====</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">'''</del>Test and characterization of device''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284022 BBa_K284022]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We concluded the construction of the device [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284022 BBa_K284022]. The results are shown next: </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We concluded the construction of the device [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284022 BBa_K284022]. The results are shown next: </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">'''</del>Cre-Recombinase without ATG's biobrick assemble<del class="diffchange diffchange-inline">'''</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">==== </ins>Cre-Recombinase without ATG's biobrick assemble <ins class="diffchange diffchange-inline">====</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The biobrick of Cre-Recombinase without the ATG start codon was successful assembled. You can check it on registry catalog under accession name [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284031 BBa_K284031]. This biobrick was also the only one we constructed according to the standard assembly strategy.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The biobrick of Cre-Recombinase without the ATG start codon was successful assembled. You can check it on registry catalog under accession name [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284031 BBa_K284031]. This biobrick was also the only one we constructed according to the standard assembly strategy.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As we can observe in the gel photo, all plasmids presented bands compatible with the size expected for the digestion.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As we can observe in the gel photo, all plasmids presented bands compatible with the size expected for the digestion.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We believe the other bands in the agarose gel represent the digested plasmid without our fragment and the circularized plasmid in different conformations.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We believe the other bands in the agarose gel represent the digested plasmid without our fragment and the circularized plasmid in different conformations.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">For more information see our lab notebook ([https://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/October_12 October 12] and [https://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/October_13 October 13])</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:UNICAMP-Brazil/inc_rodape}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:UNICAMP-Brazil/inc_rodape}}</div></td></tr>
</table>Fabihttp://2009.igem.org/wiki/index.php?title=Team:UNICAMP-Brazil/Coliguard/Results&diff=168977&oldid=prevLuige at 03:52, 22 October 20092009-10-22T03:52:27Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:New_biobrick.JPG|500px|center]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:New_biobrick.JPG|500px|center]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Agarose gel 1 %: PCR amplification and restriction enzyme analysis<del class="diffchange diffchange-inline">. </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><center></ins>Agarose gel 1 %: PCR amplification and restriction enzyme analysis <ins class="diffchange diffchange-inline"></center></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">We confirm the construction through PCR and restriction analysis. [https://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/October_14 Click here for details.]</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After the confirmation of the construction we characterized the part through the test that is described in the Notebook. [https://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/October_15#Testing_the_new_device Click here for details.]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After the confirmation of the construction we characterized the part through the test that is described in the Notebook. [https://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/October_15#Testing_the_new_device Click here for details.]</div></td></tr>
</table>Luigehttp://2009.igem.org/wiki/index.php?title=Team:UNICAMP-Brazil/Coliguard/Results&diff=168288&oldid=prevLuige at 03:36, 22 October 20092009-10-22T03:36:46Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Test and characterization of device''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284022 BBa_K284022]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Test and characterization of device''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284022 BBa_K284022]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We concluded the construction of the device [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284022 BBa_K284022].</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We concluded the construction of the device [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284022 BBa_K284022]. <ins class="diffchange diffchange-inline">The results are shown next: </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We <del class="diffchange diffchange-inline">confirmed </del>and <del class="diffchange diffchange-inline">characterized </del>the <del class="diffchange diffchange-inline">device</del>. The <del class="diffchange diffchange-inline">results are shown next: </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We <ins class="diffchange diffchange-inline">made two confirmation tests. The first test consisted in a PCR with VF2 primer forward (general primer forward backbone plasmid) </ins>and <ins class="diffchange diffchange-inline">VR primer reverse (general primer reverse backbone plasmid). We noticed </ins>the <ins class="diffchange diffchange-inline">correct band size for this amplification (833 bp)</ins>. The <ins class="diffchange diffchange-inline">second test consisted in enzyme restriction analysis with EcoRI and PstI. We noticed the right band size again (670 bp). These two tests confirmed undoubtedly the correct insertion and position of our new device. </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:New_biobrick.JPG|500px|center]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:New_biobrick.JPG|500px|center]]</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Agarose gel 1 %: PCR amplification and restriction enzyme analysis. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We confirm the construction through PCR and restriction analysis. [https://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/October_14 Click here for details.]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We confirm the construction through PCR and restriction analysis. [https://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/October_14 Click here for details.]</div></td></tr>
</table>Luigehttp://2009.igem.org/wiki/index.php?title=Team:UNICAMP-Brazil/Coliguard/Results&diff=168055&oldid=prevFabi at 03:31, 22 October 20092009-10-22T03:31:21Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Although we assembled this part’s biobrick, we didn’t have enough time to assemble the entire device, i.e., the construction with the AGTC repetition and the constitutive promoter. Therefore, we didn’t performed any experiments in order to characterize this part.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Although we assembled this part’s biobrick, we didn’t have enough time to assemble the entire device, i.e., the construction with the AGTC repetition and the constitutive promoter. Therefore, we didn’t performed any experiments in order to characterize this part.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==== PY Promoter + RFP - Construction of the device ====</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">We have confirmed the construction of our biobrick [http://partsregistry.org/Part:BBa_K284008 BBa_K284008]. This biobrick consists in the PY promoter regulating a RFP reporter.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">We designed this device to perform a conjugation experiment to characterize PY promoter and analyze its activity data in comparison with conjugation events. We want to analyze if the PY promoter activity and the conjugation begin at the same time to know if it is possible to use this promoter as a recognition mechanism.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">To confirm our part we digested it with XbaI and PstI to analyze the size of the resulting fragments in an agarose gel:</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:py_gel_10.png|300px|center]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The expected size for the fragment excised with XbaI and SpeI from the plasmid is 1028 bp.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">As we can observe in the gel photo, all plasmids presented bands compatible with the size expected for the digestion.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">We believe the other bands in the agarose gel represent the digested plasmid without our fragment and the circularized plasmid in different conformations.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:UNICAMP-Brazil/inc_rodape}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:UNICAMP-Brazil/inc_rodape}}</div></td></tr>
</table>Fabihttp://2009.igem.org/wiki/index.php?title=Team:UNICAMP-Brazil/Coliguard/Results&diff=167887&oldid=prevMbassalo: /* The Coliguard - Results */2009-10-22T03:23:39Z<p><span class="autocomment">The Coliguard - Results</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 03:23, 22 October 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The biobrick of Cre-Recombinase without the ATG start codon was successful assembled. You can check it on registry catalog under accession name [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284031 BBa_K284031]. This biobrick was also the only one we constructed according to the standard assembly strategy.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The biobrick of Cre-Recombinase without the ATG start codon was successful assembled. You can check it on registry catalog under accession name [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284031 BBa_K284031]. This biobrick was also the only one we constructed according to the standard assembly strategy.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The confirmation of it's assemble can be checked on our notebook, on [https://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/October_9 October 9th] and [https://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/October_10 October 10th].</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The confirmation of it's assemble can be checked on our notebook, on [https://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/October_9 October 9th] and [https://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/October_10 October 10th].</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Although we assembled this part’s biobrick, we didn’t have enough time to assemble the entire device, i.e., the construction with the AGTC repetition and the constitutive promoter. Therefore, we didn’t performed any experiments in order to characterize this part.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Although we assembled this part’s biobrick, we didn’t have enough time to assemble the entire device, i.e., the construction with the AGTC repetition and the constitutive promoter. Therefore, we didn’t performed any experiments in order to characterize this part.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Mbassalohttp://2009.igem.org/wiki/index.php?title=Team:UNICAMP-Brazil/Coliguard/Results&diff=167826&oldid=prevMbassalo at 03:21, 22 October 20092009-10-22T03:21:41Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Legenda.png|300px|center]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Legenda.png|300px|center]]</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">'''Cre-Recombinase without ATG's biobrick assemble'''</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The biobrick of Cre-Recombinase without the ATG start codon was successful assembled. You can check it on registry catalog under accession name [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284031 BBa_K284031]. This biobrick was also the only one we constructed according to the standard assembly strategy.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The confirmation of it's assemble can be checked on our notebook, on [https://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/October_9 October 9th] and [https://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/October_10 October 10th].</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Although we assembled this part’s biobrick, we didn’t have enough time to assemble the entire device, i.e., the construction with the AGTC repetition and the constitutive promoter. Therefore, we didn’t performed any experiments in order to characterize this part.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:UNICAMP-Brazil/inc_rodape}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:UNICAMP-Brazil/inc_rodape}}</div></td></tr>
</table>Mbassalohttp://2009.igem.org/wiki/index.php?title=Team:UNICAMP-Brazil/Coliguard/Results&diff=167317&oldid=prevAnezeidler: /* The Coliguard - Results */2009-10-22T03:07:26Z<p><span class="autocomment">The Coliguard - Results</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Graph 1. Growth cellular after ITPG <del class="diffchange diffchange-inline">induction</del>.JPG|500px|center]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Graph 1. Growth cellular after ITPG <ins class="diffchange diffchange-inline">induction1</ins>.JPG|500px|center]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Besides that, the death of the induced cells capable of expressing the endolysin was confirmed by the absence of growing colonies on the plates. On the other hand, the cultures that weren't expressing the endolysin grew normally. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Besides that, the death of the induced cells capable of expressing the endolysin was confirmed by the absence of growing colonies on the plates. On the other hand, the cultures that weren't expressing the endolysin grew normally. </div></td></tr>
</table>Anezeidlerhttp://2009.igem.org/wiki/index.php?title=Team:UNICAMP-Brazil/Coliguard/Results&diff=167292&oldid=prevAnezeidler: /* The Coliguard - Results */2009-10-22T03:06:49Z<p><span class="autocomment">The Coliguard - Results</span></p>
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</table>Anezeidlerhttp://2009.igem.org/wiki/index.php?title=Team:UNICAMP-Brazil/Coliguard/Results&diff=167029&oldid=prevAnezeidler: /* The Coliguard - Results */2009-10-22T02:59:55Z<p><span class="autocomment">The Coliguard - Results</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We confirmed that our part really works as expected!!!!</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We confirmed that our part really works as expected!!!!</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The OD measurements showed a decrease on the growth of induced strains transformed with BBa_K284022, in comparsion with induced strains transformed with BBa_K112806 (without the promoter) and the non-induced ones. The lysis of the cells expressing T4 endolysin can be visualized in the picture below. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The OD measurements showed a decrease on the growth of induced strains transformed with BBa_K284022, in comparsion with induced strains transformed with BBa_K112806 (without the promoter) and the non-induced ones. The lysis of the cells expressing T4 endolysin can be visualized in the picture below<ins class="diffchange diffchange-inline">. A graphic comparing the growing of induced and non-induced cultures after the induction is also shown</ins>. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:cell-lysis.png|500px|center]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:cell-lysis.png|500px|center]]</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:Graph 1. Growth cellular after ITPG induction.JPG|500px|center]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Besides that, the death of the induced cells capable of expressing the endolysin was confirmed by the absence of growing colonies on the plates. On the other hand, the cultures that weren't expressing the endolysin grew normally. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Besides that, the death of the induced cells capable of expressing the endolysin was confirmed by the absence of growing colonies on the plates. On the other hand, the cultures that weren't expressing the endolysin grew normally. </div></td></tr>
</table>Anezeidlerhttp://2009.igem.org/wiki/index.php?title=Team:UNICAMP-Brazil/Coliguard/Results&diff=166787&oldid=prevAnezeidler: /* The Coliguard - Results */2009-10-22T02:55:04Z<p><span class="autocomment">The Coliguard - Results</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>It was also possible to observe the overexpression of T4 endolysin through an SDS-PAGE.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>It was also possible to observe the overexpression of T4 endolysin through an SDS-PAGE.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:UNICAMP-Brazil/inc_rodape}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:UNICAMP-Brazil/inc_rodape}}</div></td></tr>
</table>Anezeidler