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Team:UNICAMP-Brazil/Notebooks/August 15
From 2009.igem.org
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(New page: - Today we started the construction of our first biobrick. After quantificate the mini-prep's products we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP re...) |
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- Today we started the construction of our first biobrick. After quantificate the mini-prep's products we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter. | - Today we started the construction of our first biobrick. After quantificate the mini-prep's products we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter. | ||
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- The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today. | - The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today. | ||
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| + | ''Raíssa'' | ||
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| + | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Revision as of 16:52, 5 September 2009
YeastGuard: Digestion of CFP and RBS
- Today we started the construction of our first biobrick. After quantificate the mini-prep's products we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter.
- After the digestion we isolated the target bands from the agarose gel, according to the protocol X.
- The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today.
Raíssa
