Team:UNICAMP-Brazil/Notebooks/August 15

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Revision as of 16:53, 5 September 2009

YeastGuard: RBS and CFP plasmid's digestion

- Today we started the construction of our first biobrick. After quantificate the mini-prep's products we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter.

- After the digestion we isolated the target bands from the agarose gel, according to the protocol X.

- The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today.

Raíssa

Revision as of 16:53, 5 September 2009 (view source) Raissa (Talk \| contribs) ← Older edit		Revision as of 16:53, 5 September 2009 (view source) Raissa (Talk \| contribs) (→YeastGuard: recovering BBa_J63002) Newer edit →
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-	== YeastGuard: ~~recovering BBa_J63002~~==	+	== YeastGuard: RBS and CFP plasmid's digestion ==

	- Today we started the construction of our first biobrick. After quantificate the mini-prep's products we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter.		- Today we started the construction of our first biobrick. After quantificate the mini-prep's products we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter.