Team:UNICAMP-Brazil/Notebooks/August 15

From 2009.igem.org

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(YeastGuard: recovering BBa_J63002)
(YeastGuard: RBS and CFP plasmid's digestion)
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== YeastGuard: RBS and CFP plasmid's digestion ==
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== YeastGuard: RBS and CFP plasmid digestion ==
- Today we started the construction of our first biobrick. After quantificate the mini-prep's products we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter.  
- Today we started the construction of our first biobrick. After quantificate the mini-prep's products we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter.  

Revision as of 16:54, 5 September 2009

YeastGuard: RBS and CFP plasmid digestion

- Today we started the construction of our first biobrick. After quantificate the mini-prep's products we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter.

- After the digestion we isolated the target bands from the agarose gel, according to the protocol X.

- The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today.

Raíssa