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Team:UNICAMP-Brazil/Notebooks/August 17
From 2009.igem.org
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(New page: == YeastGuard: Trying to purify the RBS digested fragment == - The RBS band purification performed on August 15 didn't work, so we digested the plasmid again. Curiously the digested band ...) |
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- After this finding we checked the other biobricks and ressuspended the correct RBS one. We transformed it in ''E. coli'', plated and grew it in liquid media. | - After this finding we checked the other biobricks and ressuspended the correct RBS one. We transformed it in ''E. coli'', plated and grew it in liquid media. | ||
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Revision as of 17:09, 5 September 2009
YeastGuard: Trying to purify the RBS digested fragment
- The RBS band purification performed on August 15 didn't work, so we digested the plasmid again. Curiously the digested band was a little bigger than we expected, so we decided to check over it's size on Registry and we found that we ressuspended the wrong biobrick!!! Fortunatly it's purification didn't work before! =)
- After this finding we checked the other biobricks and ressuspended the correct RBS one. We transformed it in E. coli, plated and grew it in liquid media.
Raíssa
