Team:UNICAMP-Brazil/Notebooks/August 17

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YeastGuard: Trying to purify the RBS digested fragment

- The RBS band purification performed on August 15 didn't work, so we digested the plasmid again. Curiously the digested band was a little bigger than we expected, so we decided to check over it's size on Registry and we found that we ressuspended the wrong biobrick!!! Fortunatly it's purification didn't work before! =)

- After this finding we checked the other biobricks and ressuspended the correct RBS one. We transformed it in E. coli, plated and grew it in liquid media.

Raíssa




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