Team:UNICAMP-Brazil/Notebooks/July 24

From 2009.igem.org

Revision as of 20:22, 10 October 2009 by Victor.negri (Talk | contribs)

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

ColiGuard

R Plasmid Minipreps

  • Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process).
  • We performed minipreps from R plasmid-containing cultures, according to Protocol 2.

As we did for F plasmid, we were also able to recover the R plasmid.

Gabriel, Marcelo and Victor.