Team:UNICAMP-Brazil/Notebooks/October 20

From 2009.igem.org

(Difference between revisions)
(Yeast experiments: Lysozyme)
(Yeast experiments: Lysozyme)
Line 9: Line 9:
====Yeast experiments: Lysozyme====
====Yeast experiments: Lysozyme====
-
*<p style=”text-align:justify;”>Today we began to test the yeasts transformed with the following constructions: YEP+pDLD-Lysozyme and YEP+Adh1-Lysozyme.</p>
+
*<p style=”text-align:justify;”>Today we began the tests with the yeasts transformed with the following constructions: YEP+pDLD-Lysozyme and YEP+Adh1-Lysozyme.</p>
*<p style=”text-align:justify;”>We inoculated the yeast pre inocula in 3 types of media: YNB Glucose Ura-, YNB Ethanol Ura- and YNB Glucose Ura+. The inocula grew for 4 hours in 30ºC and 150rpm before the induction with lactic acid. Four concentrations of lactic acid were used in the induction.</p>
*<p style=”text-align:justify;”>We inoculated the yeast pre inocula in 3 types of media: YNB Glucose Ura-, YNB Ethanol Ura- and YNB Glucose Ura+. The inocula grew for 4 hours in 30ºC and 150rpm before the induction with lactic acid. Four concentrations of lactic acid were used in the induction.</p>

Revision as of 22:07, 21 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

YeastGuard

Yeast experiments: Lysozyme

  • Today we began the tests with the yeasts transformed with the following constructions: YEP+pDLD-Lysozyme and YEP+Adh1-Lysozyme.

  • We inoculated the yeast pre inocula in 3 types of media: YNB Glucose Ura-, YNB Ethanol Ura- and YNB Glucose Ura+. The inocula grew for 4 hours in 30ºC and 150rpm before the induction with lactic acid. Four concentrations of lactic acid were used in the induction.


Raíssa and Taís