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Team:UNICAMP-Brazil/Notebooks/October 20
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*<p style=”text-align:justify;”>Today we began the tests with the yeasts transformed with the following constructions: YEP+pDLD-Lysozyme and YEP+Adh1-Lysozyme.</p> | *<p style=”text-align:justify;”>Today we began the tests with the yeasts transformed with the following constructions: YEP+pDLD-Lysozyme and YEP+Adh1-Lysozyme.</p> | ||
| - | * | + | * The pre inocula made yesterday grew. We inoculated it in 50mL of YNB Glucose Ura- or YNB Ethanol Ura-. The wild type yeast is Ura- so we grew it on YNB Glucose Ura+. The inocula grew for 4 hours in 30ºC and 150rpm until the OD 1,3 before the induction with lactic acid. |
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| + | * We performed the induction 5 hours long. We mesured the OD every one hour to construct a growth curve. At the end of the 5 hours we pelleted the culture, colected the supernatant and freezed the pellet to analyze in SDS-PAGE tomorrow. | ||
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| + | * We also dripped drops of the supernatant in filter paper discs and placed them in a ''Lactococcus lactis'' plate. We hope to see inhibition zones surrounding some of the filters. | ||
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| + | * The Yeasts inoculated in YNB Ethanol media didn't grow enough to begin the induction until the end of the day. | ||
Revision as of 22:23, 21 October 2009
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