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Team:UNICAMP-Brazil/Notebooks/October 4
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(New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' ColiGuard '''== ====finOP and Cre-Recombinase with pGEM strategy==== * Yestarday's transformed ...) |
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====finOP and Cre-Recombinase with pGEM strategy==== | ====finOP and Cre-Recombinase with pGEM strategy==== | ||
| - | * | + | * Yesterday's transformed and plated cells grew in the media! We found white and blue colonies in the plate just as expected (due to the beta-galactosidase gene contained in pGEM vector). |
* We selected 10 white colonies (those theorically contains our inserts in the correct position, since it interrupts the coding sequence of beta-galactosidase) from each plate and inoculated them into liquid LB-AMP media. | * We selected 10 white colonies (those theorically contains our inserts in the correct position, since it interrupts the coding sequence of beta-galactosidase) from each plate and inoculated them into liquid LB-AMP media. | ||
* Inocula were keep at 37ºC, under 250 rpm, for an O/N period. | * Inocula were keep at 37ºC, under 250 rpm, for an O/N period. | ||
Revision as of 20:13, 10 October 2009
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