Team:UNICAMP-Brazil/Notebooks/October 6

From 2009.igem.org

Revision as of 21:22, 20 October 2009 by Marcos Moraes (Talk | contribs)

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

ColiGuard

Another Miniprep, another digestion, another ligation and once again, another transformation

Transformation of the BBa K112806 + BBa B0015 ligation

  • We did the miniprep of BBa B0015 and BBa K112806 according to Protocol 2

  • With the protduct of the miniprep we made the digestion of BBa B0015 with EcoRI and XbaI and with EcoRI and SpeI to BBa K112806. After 3 hours of digestion we purified BBa K112806 from agarose gel and BBa B0015 directly from the digestion reaction.

  • Using the purified digestions we did the ligation, Protocol 11, of BBa B0015 with BBa K112806 and to test the digestion of BBa B0015 we did a ligation only without BBa K112806, if colonies grow with this ligation it means BBa B0015 is recircularizing. We staterd to quantify our samples with the new lab's espectrophotometer.

  • We transformed E. coli with the ligation, we hope this time it works!!

Ane and Marcos

YeastGuard

New biobricks - New strategy

- We inoculated Jen1orf-pGEM and Jen1promoter-pGEM transformed colonies in liquid media to perform the screening tomorrow.

- We need more parts!! We permormed PCR to obtain the following parts: End, Jen1 orf and promoter Jen1. Unfortunately only Jen1 orf amplified.

GEL!!

Raíssa