After the O/N period, today we transformed the ligation finO+pSB1A3 into electrocompetent E. coli bacteria, strain DH10B. We followed protocol 3, without modifications (see Protocols section).
We then plated the transfomed cells in LB-AMP media, and let them grow at 37ºC for an O/N period.
finP+pSB1A3
We followed the same produce for the finP+pSB1A3 ligation.
Marcelo
YeastGuard
==New biobricks in biobrick format==
We performed miniprep to get purified the plasmids containing these parts. (Protocol 2).
We then digest the plasmids obtained by the miniprep with XbaI to confirm the correct ligation of the parts following the biobrick format (Protocolo x). However, the electrophoresis gel (down) showed no success in the ligations.
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