Team:UNICAMP-Brazil/Notebooks/September 19
From 2009.igem.org
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''Marcelo'' | ''Marcelo'' | ||
+ | ====Cre-Recombinase + pSB1A3 ligation==== | ||
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+ | *<p style=”text-align:justify;”>Once we digested Cre-Recombinase's insert and the vector with XbaI and SpeI restriction enzymes, we could perform the ligation between them, according to protocol [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11].</p> | ||
+ | *<p style=”text-align:justify;”>Then, we transformed the ligation into electrocompetent 'E. coli' bacteria, strain DH10B, accordin to protocol [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporation Protocol 3].</p> | ||
+ | *<p style=”text-align:justify;”>After being incubated at 37ºC for 1 hour, transformed cells were plated into solid LB-AMP media, and were grown at 37ºC for an O/N period.</p> | ||
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+ | ''Víctor'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} |
Revision as of 06:21, 21 October 2009
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