Team:UNICAMP-Brazil/Yeastguard/Recognition
From 2009.igem.org
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==Recognition of lactate== | ==Recognition of lactate== | ||
- | <p style=”text-align:justify;”>The ideal device to sense the presence of lactate in the yeasts´ cytoplasm and activate the killing mechanism must respond to lactate and must not be repressed by glucose. Thus, two promoters were chosen as candidates (2,3): ''DLD'' (modified) and ''JEN1''; both found in the genome of the yeast ''Kluyveromyces lactis var. lactis''. These promoters were amplified by PCR from the genome of ''K. lactis'' and will include the sites upstream the ATG. The amplicons have approximately 1000bp (JEN1) and 471bp (DLD).</p> | + | <p style=”text-align:justify;”>The ideal device to sense the presence of lactate in the yeasts´ cytoplasm and activate the killing mechanism must respond to lactate and must not be repressed by glucose. Thus, two promoters were chosen as candidates (2,3): ''DLD'' (modified) and ''JEN1''; both found in the genome of the yeast ''Kluyveromyces lactis var. lactis''. These promoters were amplified by PCR from the genome of ''K. lactis'' and will include the sites upstream the ATG. The amplicons have approximately 1000bp (''JEN1'') and 471bp (''DLD'').</p> |
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<p style="text-align:justify;font-size:0.9em;">Figure 2: JEN1 and DLD promoters from ''Kluyveromyces lactis''.</p> | <p style="text-align:justify;font-size:0.9em;">Figure 2: JEN1 and DLD promoters from ''Kluyveromyces lactis''.</p> | ||
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==Caracterization of the Biobricks== | ==Caracterization of the Biobricks== |
Revision as of 00:21, 22 October 2009
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