Team:UQ-Australia/Notebook
From 2009.igem.org
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'''Digest Gel of BBa_I716101 with pGfa2cLac2 control'''<br/> | '''Digest Gel of BBa_I716101 with pGfa2cLac2 control'''<br/> | ||
- | A 1% agarose gel was run with 1kb ladder and EcoRI, XhoI, BglII and BamHI restriction enzymes. The gel visualisation (image here) indicates that only EcoRI was able to slice the plasmid; it is possible that the other restriction sites are not on the plasmid.<br/> | + | A 1% agarose gel was run with 1kb ladder and samples with EcoRI, XhoI, BglII and BamHI restriction enzymes. The gel visualisation (image here) indicates that only EcoRI was able to slice the plasmid; it is possible that the other restriction sites are not on the plasmid.<br/> |
- | '''25/08/09 - | + | '''25/08/09 - In-gel Extraction and Overnight Digests'''<br/> |
- | + | 20 chloroamphenicol plates were prepared with LB agar. BBa_I716101 and control plasmid pGfa2cLac2 were each digested with EcoRI, XhoI, BglII and BamHI enzymes (total of 8 samples). Digests have been left to run overnight in the 37C waterbath. | |
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Revision as of 05:28, 3 September 2009
Water Purification Project02/09/09 - Running Gel for Standard 23
EDIT (01/09/09): these tubes now in IGEM Green Box ("Fiona" freezer, bottom shelf).
27/08/09 - Colony Picking 26/08/09 - Transformation of Plasmid BBa_J63010 with part BBa_J04450 Digest Gel of BBa_I716101 with pGfa2cLac2 control 25/08/09 - In-gel Extraction and Overnight Digests 24/07/09 - DNA extraction and electrophoresis 23/07/09 - Transformations with AG43 plasmids Strain: MS427
Strain: MS427 17/07/09 - MG1655 Stock Preparation
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Bioprecipitation Project02/09/09 For the last couple of weeks we have been trying to design primers in order to put them into the iGEM standard plasmids. Since restriction sites are against us, we are using mutagenesis in order to bypass this problem. Primers were ordered TODAY! 19/08/09 Miniprep of bacterial cultures. For procedure click HERE. Plasmids were stored in -20'C freezer (FIONA). 18/08/09 Colonies were picked from transformed bacteria. Protocol can be found by clicking HERE. 14/08/09
Plates were sealed with parafilm and stored in the -4 fridge. 13/08/09 A second transformation was performed on the four plasmids. Procedure slightly changed, click HERE to see it. 11/08/09 No results were given from the transformation. Transformation will have to be repeated.
To see protocol, click HERE
LB media was poured on petri dishes and plasmid DNA was checked again using Nanodrop.
Plasmids were stored and transformation will be done on Monday.
Plasmids have arrived! We have put them into 150 mL of TE buffer and left overnight for the paper to dissolve and later extract the plasmid from the solution. A Nanodrop was used to see if the DNA was coming out of the paper. No DNA was detected, thus samples were left overnight. Click on the plasmid name to look at the vector |