Team:UQ-Australia/Notebook/Experiment1

From 2009.igem.org

(Difference between revisions)
(Miniprep Procedure)
(Gel Extraction for PCR product of AG43)
 
Line 415: Line 415:
Follow the procedure described in Quick Gel Extraction Kit Manual (Click HERE for more details)
Follow the procedure described in Quick Gel Extraction Kit Manual (Click HERE for more details)
Result: Nanodrop  of gel extraction ‘s final products:
Result: Nanodrop  of gel extraction ‘s final products:
-
Sample name -- Concentration (ng/uL) -- 260/280
+
*Sample name -- Concentration (ng/uL) -- 260/280
-
PCR1 -- 40.04 -- 1.99
+
*PCR1 -- 40.04 -- 1.99
-
PCR2 -- 27.05 -- 1.96
+
*PCR2 -- 27.05 -- 1.96

Latest revision as of 12:32, 20 October 2009

Gel Extraction for PCR product of AG43

Running PCR products of Ag43 collected from reaction1 (named PCR1) and 2 (named PCR2) (Click HERE for further details on 06/10/09 ) on 1% agarose gel. Click HERE for the gel image. (File 4)

Follow the procedure described in Quick Gel Extraction Kit Manual (Click HERE for more details) Result: Nanodrop of gel extraction ‘s final products:

  • Sample name -- Concentration (ng/uL) -- 260/280
  • PCR1 -- 40.04 -- 1.99
  • PCR2 -- 27.05 -- 1.96