Team:Uppsala-Sweden/Modelling
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{{Uppsala-Sweden Template}} | {{Uppsala-Sweden Template}} | ||
- | ==Modelling of | + | ==Modelling of Advanced Ethanol Production== |
For our modell we have some basic assumptions that are | For our modell we have some basic assumptions that are | ||
- | + | # Co-evolution of the of the ethanol producing pathway, | |
making km and kcat being highly similar for pdc as well as | making km and kcat being highly similar for pdc as well as | ||
ADH | ADH | ||
- | + | # Assuming quasi steady state (Michaelis Menten Kinetics) | |
[[Image:ek_0.png]] | [[Image:ek_0.png]] | ||
Line 23: | Line 23: | ||
- | + | # Having e0 and letting kt go towards zero, (kt here represents the rate at which our substrate pyruvate "disappears" into the Krebbs cycle), gives ks | |
[[Image:ek_4.png]] | [[Image:ek_4.png]] | ||
- | + | # Assuming Ks to be constant within the interval and assuming steady-state | |
[[Image:ek_5.png]] | [[Image:ek_5.png]] |
Revision as of 18:19, 20 October 2009
Modelling of Advanced Ethanol Production
For our modell we have some basic assumptions that are
- Co-evolution of the of the ethanol producing pathway,
making km and kcat being highly similar for pdc as well as ADH
- Assuming quasi steady state (Michaelis Menten Kinetics)
Having the Km and Kcat values gives e0 from level of product formation.
- Having e0 and letting kt go towards zero, (kt here represents the rate at which our substrate pyruvate "disappears" into the Krebbs cycle), gives ks
- Assuming Ks to be constant within the interval and assuming steady-state
S is a function of f(x,y) where x is proportional to e0 and y is proportional to kt
Measure for different x and y and fit the function