Team:Uppsala-Sweden/Notebook

From 2009.igem.org

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{{Uppsala-Sweden Template}}
{{Uppsala-Sweden Template}}
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==Oligonucleotides used by the Uppsala iGEM Team 2009==
 
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All primers we used during the project can be found here: [[Media:iGEMUppsala09_Primer.xls]]<br>
 
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Annealing temperatures etc. can be found in the notebook (see below).
 
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==Protocols==
 
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===PCR Setup===
 
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[[Media:PCR MM and Primers.xls]]
 
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===Transformation Protocol===
 
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[[Media:Transformation workorder.pdf]]
 
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===Growth media for <i>Z. mobilis</i>===
 
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[[Media:Fullmedium mobilis.xls]]<br>
 
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[[Media:Fullmedium mobilis plus Agar.xls]]
 
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===Growth medium for <i>Synechocystis sp PCC 6803</i>===
 
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[[Media:BG11.pdf]]
 
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===Growth medium for <i>E. coli</i>===
 
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[[Media:LB.pdf]]
 
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===Various===
 
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General Glycerol Stock Protocol:<br>
 
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[[Media:Glycerol Stocks.xls]]
 
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== '''Notebook''' ==
 
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==Notebook==
{| align="center"
{| align="center"
|-valign="top"
|-valign="top"
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|}
|}
 +
 +
 +
==Oligonucleotides used by the Uppsala iGEM Team 2009==
 +
All primers we used during the project can be found here: [[Media:iGEMUppsala09_Primer.xls|iGEMUppsala09_Primer.xls]]<br>
 +
Annealing temperatures etc. can be found in the notebook.
 +
 +
==Protocols==
 +
 +
====PCR Setup====
 +
[[Media:PCR MM and Primers.xls|PCR MM and Primers.xls]]
 +
 +
====Transformation Protocol====
 +
[[Media:Transformation workorder.pdf|Transformation workorder.pdf]]
 +
 +
====Growth media for <i>Z. mobilis</i>====
 +
[[Media:Fullmedium mobilis.xls|Fullmedium mobilis.xls]]<br>
 +
[[Media:Fullmedium mobilis plus Agar.xls|Fullmedium mobilis plus Agar.xls]]
 +
 +
====Growth medium for <i>Synechocystis sp PCC 6803</i>====
 +
[[Media:BG11.pdf|BG11.pdf]]
 +
 +
====Growth medium for <i>E. coli</i>====
 +
[[Media:LB.pdf|LB.pdf]]
 +
 +
====Various====
 +
[[Media:Glycerol Stocks.xls|Glycerol Stocks.xls]]<br>
 +
[[Media:Triparental_Mating.pdf|Triparental_Mating.pdf]]
 +
 +
__NOTOC__
{{Uppsala-Sweden_Footer}}
{{Uppsala-Sweden_Footer}}

Latest revision as of 02:42, 22 October 2009




Notebook

June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31


Oligonucleotides used by the Uppsala iGEM Team 2009

All primers we used during the project can be found here: iGEMUppsala09_Primer.xls
Annealing temperatures etc. can be found in the notebook.

Protocols

PCR Setup

PCR MM and Primers.xls

Transformation Protocol

Transformation workorder.pdf

Growth media for Z. mobilis

Fullmedium mobilis.xls
Fullmedium mobilis plus Agar.xls

Growth medium for Synechocystis sp PCC 6803

BG11.pdf

Growth medium for E. coli

LB.pdf

Various

Glycerol Stocks.xls
Triparental_Mating.pdf






Bioneer Biolegio Clontech Uppsala Genome Center