Team:Uppsala-Sweden/Parts

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{{Uppsala-Sweden Template}}
{{Uppsala-Sweden Template}}
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==Parts==
+
<html>
 +
<body>
 +
  <table id="toc" class="toc" summary="Contents"><tr><td><div id="toctitle">Contents</h2></div>
 +
  <ul>
 +
    <li class="toclevel-1"><a href="#reg"><span class="tocnumber">1</span> <span class="toctext">Regulatory Elements</span></a>
 +
    <ul>
 +
    <li class="toclevel-2"><a href="#pro"><span class="tocnumber">2.1</span> <span class="toctext">Promoter</span></a></li>
 +
  </ul>
 +
  </li>
 +
    <li class="toclevel-1"><a href="#prod"><span class="tocnumber">2</span> <span class="toctext">Alcohol Production</span></a>
 +
  <ul>
 +
    <li class="toclevel-2"><a href="#but"><span class="tocnumber">2.1</span> <span class="toctext">Butanol Parts, Intermediates and Devices</span></a></li>
 +
    <li class="toclevel-2"><a href="#eth1"><span class="tocnumber">2.2</span> <span class="toctext">Ethanol Parts, Intermediates and Devices</span></a></li>
 +
    <li class="toclevel-2"><a href="#eth2"><span class="tocnumber">2.3</span> <span class="toctext">Ethanol Parts - S. cerevisae</span></a></li>
 +
  </ul>
 +
    </li>
 +
    <li class="toclevel-1"><a href="#pyr"><span class="tocnumber">3</span> <span class="toctext">Pyruvate Level Increasing Approaches</span></a>
 +
  <ul>
 +
    <li class="toclevel-2"><a href="#pir"><span class="tocnumber">3.1</span> <span class="toctext">Pir Approach</span></a></li>
 +
    <li class="toclevel-2"><a href="#rna"><span class="tocnumber">3.2</span> <span class="toctext">Antisense RNA Approach</span></a></li>
 +
  </ul>
 +
  </li>
 +
  </ul>
 +
  </td></tr></table><script type="text/javascript"> if (window.showTocToggle) { var tocShowText = "show"; var tocHideText = "hide"; showTocToggle(); } </script></body></html>
 +
 
 +
 
 +
=Parts=
 +
Here we list all the BioBricks we designed, constructed and used during the project.
 +
 
 +
<html>
 +
<body>
 +
  <TABLE WIDTH="100%" BORDER="0" CELLPADDING="1">
 +
  <TR>
 +
    <TD colspan="4">
 +
    <a class="ah1" name="reg">Regulatory Elements</a><br><br>
 +
    </TD>
 +
  </TR>
 +
    <TR>
 +
    <TD colspan="4">
 +
    <a class="ah3" name="pro">Promoter</a><br>
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273019">BBa_K273019</a>
 +
    </TD>
 +
    <TD>
 +
    Regulatory
 +
    </TD>
 +
    <TD>
 +
    pPetE
 +
    </TD>
 +
    <TD>
 +
    429
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
  </TR>
 +
    <TR>
 +
    <TD colspan="4">
 +
    <br><a class="ah1" name="prod">Alcohol Production</a><br><br>
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD colspan="4">
 +
    <a class="ah3" name="but">Butanol Parts, Intermediates and Devices</a><br>
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273006">BBa_K273006</a>
 +
    </TD>
 +
    <TD>
 +
    Coding
 +
    </TD>
 +
    <TD>
 +
    a-ketoisovalerate decarboxylase (kivd) codon optimized
 +
    </TD>
 +
 
 +
    <TD>
 +
    1650
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273008">BBa_K273008</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    Intermediate
 +
    </TD>
 +
    <TD>
 +
    RBS - a-ketoisovalerate decarboxylase (kivd) codon optimized
 +
    </TD>
 +
    <TD>
 +
    1668
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273005">BBa_K273005</a>
 +
    </TD>
 +
    <TD>
 +
    Coding
 +
    </TD>
 +
    <TD>
 +
    Alcohol Dehydrogenase 2 (Yadh2)
 +
    </TD>
 +
 
 +
    <TD>
 +
    1050
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273007">BBa_K273007</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    Intermediate
 +
    </TD>
 +
    <TD>
 +
    RBS - Alcohol Dehydrogenase 2 (Yadh2)
 +
    </TD>
 +
    <TD>
 +
    1068
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273009">BBa_K273009</a>
 +
    </TD>
 +
    <TD>
 +
    Intermediate
 +
    </TD>
 +
    <TD>
 +
    RBS - adh2(Y) - Terminator
 +
    </TD>
 +
 
 +
    <TD>
 +
    1205
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273010"><a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273012">BBa_K273012</a></a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    Intermediate
 +
    </TD>
 +
    <TD>
 +
    RBS - kivd - RBS - adh2 - Ter
 +
    </TD>
 +
    <TD>
 +
    2881
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273011">BBa_K273011</a>
 +
    </TD>
 +
    <TD>
 +
    Composite
 +
    </TD>
 +
    <TD>
 +
    PLlac - RBS - kivd - RBS - adh2(Y) - Ter
 +
    </TD>
 +
 
 +
    <TD>
 +
    2944
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273012">BBa_K273012</a>
 +
    </TD>
 +
    <TD>
 +
    Composite
 +
    </TD>
 +
    <TD>
 +
    pPetE - RBS - kivd - RBS - adh2(Y) - Ter
 +
    </TD>
 +
    <TD>
 +
    3318
 +
    </TD>
 +
    <TD>
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
  </TR>
 +
  <TR>
 +
    <TD colspan="4">
 +
    <br><a class="ah3" name="eth1">Ethanol Parts and Devices</a><br>
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273000">BBa_K273000</a>
 +
    </TD>
 +
    <TD>
 +
    Coding
 +
    </TD>
 +
    <TD>
 +
    Pyruvate decarboxylase (pdc)
 +
    </TD>
 +
 
 +
    <TD>
 +
    1710
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273001">BBa_K273001</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    Coding
 +
    </TD>
 +
    <TD>
 +
    Alcohol dehydrogenase (Zadh2)
 +
    </TD>
 +
    <TD>
 +
    1155
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273004">BBa_K273004</a>
 +
    </TD>
 +
    <TD>
 +
    Intermediate
 +
    </TD>
 +
    <TD>
 +
    RBS - Pyruvate decarboxylase (pdc)
 +
    </TD>
 +
 
 +
    <TD>
 +
    1728
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273017">BBa_K273017</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    Intermediate
 +
    </TD>
 +
    <TD>
 +
    RBS - Alcohol dehydrogenase (Zadh2)
 +
    </TD>
 +
    <TD>
 +
    1173
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273018">BBa_K273018</a>
 +
    </TD>
 +
    <TD>
 +
    Intermediate
 +
    </TD>
 +
    <TD>
 +
    RBS - adh2(Z) - Ter
 +
    </TD>
 +
 
 +
    <TD>
 +
    1310
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273013">BBa_K273013</a>
 +
    </TD>
 +
    <TD>
 +
    Intermediate
 +
    </TD>
 +
 
 +
    <TD>
 +
    RBS - pdc - RBS -adh2(Z) - Ter
 +
    </TD>
 +
    <TD>
 +
    3046
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273056">BBa_K273056</a>
 +
 
 +
    </TD>
 +
    <TD>
 +
    Composite
 +
    </TD>
 +
    <TD>
 +
    PLlac - RBS - pdc - RBS - adh2(Z) - Ter
 +
    </TD>
 +
    <TD>
 +
    3109
 +
    </TD>
 +
 
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273057">BBa_K273057</a>
 +
    </TD>
 +
    <TD>
 +
    Composite
 +
    </TD>
 +
    <TD>
 +
 
 +
    pPetE - RBS - pdc - RBS - adh2(Z) - Ter
 +
    </TD>
 +
    <TD>
 +
    3483
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
  </TR>
 +
  <TR>
 +
    <TD colspan="4">
 +
    <br><a class="ah3" name="eth2">Ethanol - <i>S. cerevisae</i></a><br>
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273014">BBa_K273014</a>
 +
    </TD>
 +
    <TD>
 +
    Intermediate
 +
    </TD>
 +
 
 +
    <TD>
 +
    RBS - pdc - RBS - adh2(Y) - Ter
 +
    </TD>
 +
    <TD>
 +
    2941
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273015">BBa_K273015</a>
 +
 
 +
    </TD>
 +
    <TD>
 +
    Composite
 +
    </TD>
 +
    <TD>
 +
    PLlac - RBS - pdc - RBS - adh2(Y) - Ter
 +
    </TD>
 +
    <TD>
 +
    3004
 +
    </TD>
 +
 
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273016">BBa_K273016</a>
 +
    </TD>
 +
    <TD>
 +
    Composite
 +
    </TD>
 +
    <TD>
 +
 
 +
    pPetE - RBS - pdc - RBS - adh2(Y) - Ter
 +
    </TD>
 +
    <TD>
 +
    3378
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
  </TR>
 +
  <TR>
 +
    <TD colspan="4">
 +
    <br><a class="ah1" name="pyr">Pyruvate Level Increasing Approaches</a><br><br>
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD colspan="4">
 +
    <a class="ah3" name="pir">Pir Approach</a><br>
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
 
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273002">BBa_K273002</a>
 +
    </TD>
 +
    <TD>
 +
    Coding
 +
    </TD>
 +
    <TD>
 +
    Pirin like protein (pirA)
 +
    </TD>
 +
    <TD>
 +
 
 +
    702
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273003">BBa_K273003</a>
 +
    </TD>
 +
    <TD>
 +
    Coding
 +
    </TD>
 +
 
 +
    <TD>
 +
    Pirin like protein (pirB)
 +
    </TD>
 +
    <TD>
 +
    240
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
  </TR>
 +
  <TR>
 +
    <TD colspan="4">
 +
    <br><a class="ah3" name="rna">Antisense RNA Approach</a><br>
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273020">BBa_K273020</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    RNA
 +
    </TD>
 +
    <TD>
 +
    E1A1
 +
    </TD>
 +
    <TD>
 +
    20
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273021">BBa_K273021</a>
 +
    </TD>
 +
    <TD>
 +
    RNA
 +
    </TD>
 +
    <TD>
 +
    E1A2
 +
    </TD>
 +
 
 +
    <TD>
 +
    30
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273022">BBa_K273022</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    RNA
 +
    </TD>
 +
    <TD>
 +
    E1A3
 +
    </TD>
 +
    <TD>
 +
    50
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273023">BBa_K273023</a>
 +
    </TD>
 +
    <TD>
 +
    RNA
 +
    </TD>
 +
    <TD>
 +
    E1A4
 +
    </TD>
 +
 
 +
    <TD>
 +
    30
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273024">BBa_K273024</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    RNA
 +
    </TD>
 +
    <TD>
 +
    E1B1
 +
    </TD>
 +
    <TD>
 +
    30
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273025">BBa_K273025</a>
 +
    </TD>
 +
    <TD>
 +
    RNA
 +
    </TD>
 +
    <TD>
 +
    E1B2
 +
    </TD>
 +
 
 +
    <TD>
 +
    30
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273026">BBa_K273026</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    RNA
 +
    </TD>
 +
    <TD>
 +
    E1B3
 +
    </TD>
 +
    <TD>
 +
    20
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273027">BBa_K273027</a>
 +
    </TD>
 +
    <TD>
 +
    RNA
 +
    </TD>
 +
    <TD>
 +
    E1B4
 +
    </TD>
 +
 
 +
    <TD>
 +
    37
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273028">BBa_K273028</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    RNA
 +
    </TD>
 +
    <TD>
 +
    E21
 +
    </TD>
 +
    <TD>
 +
    30
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273029">BBa_K273029</a>
 +
    </TD>
 +
    <TD>
 +
    RNA
 +
    </TD>
 +
    <TD>
 +
    E22
 +
    </TD>
 +
 
 +
    <TD>
 +
    30
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273030">BBa_K273030</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    RNA
 +
    </TD>
 +
    <TD>
 +
    E23
 +
    </TD>
 +
    <TD>
 +
    20
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273031">BBa_K273031</a>
 +
    </TD>
 +
    <TD>
 +
    RNA
 +
    </TD>
 +
    <TD>
 +
    E24
 +
    </TD>
 +
 
 +
    <TD>
 +
    50
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273032">BBa_K273032</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    Intermediate
 +
    </TD>
 +
    <TD>
 +
    E1A1 - Ter
 +
    </TD>
 +
    <TD>
 +
    157
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273033">BBa_K273033</a>
 +
    </TD>
 +
    <TD>
 +
    Intermediate
 +
    </TD>
 +
    <TD>
 +
    E1A2 - Ter
 +
    </TD>
 +
 
 +
    <TD>
 +
    167
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273034">BBa_K273034</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    Intermediate
 +
    </TD>
 +
    <TD>
 +
    E1A3 - Ter
 +
    </TD>
 +
    <TD>
 +
    187
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273035">BBa_K273035</a>
 +
    </TD>
 +
    <TD>
 +
    Intermediate
 +
    </TD>
 +
    <TD>
 +
    E1A4 - Ter
 +
    </TD>
 +
 
 +
    <TD>
 +
    167
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273036">BBa_K273036</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    Intermediate
 +
    </TD>
 +
    <TD>
 +
    E1B1 - Ter
 +
    </TD>
 +
    <TD>
 +
    167
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273037">BBa_K273037</a>
 +
    </TD>
 +
    <TD>
 +
    Intermediate
 +
    </TD>
 +
    <TD>
 +
    E1B2 - Ter
 +
    </TD>
 +
 
 +
    <TD>
 +
    167
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273038">BBa_K273038</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    Intermediate
 +
    </TD>
 +
    <TD>
 +
    E1B3 - Ter
 +
    </TD>
 +
    <TD>
 +
    157
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273039">BBa_K273039</a>
 +
    </TD>
 +
    <TD>
 +
    Intermediate
 +
    </TD>
 +
    <TD>
 +
    E1B4 - Ter
 +
    </TD>
 +
 
 +
    <TD>
 +
    174
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273040">BBa_K273040</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    Intermediate
 +
    </TD>
 +
    <TD>
 +
    E21 - Ter
 +
    </TD>
 +
    <TD>
 +
    167
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273041">BBa_K273041</a>
 +
    </TD>
 +
    <TD>
 +
    Intermediate
 +
    </TD>
 +
    <TD>
 +
    E22 - Ter
 +
    </TD>
 +
 
 +
    <TD>
 +
    167
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273042">BBa_K273042</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    Intermediate
 +
    </TD>
 +
    <TD>
 +
    E23 - Ter
 +
    </TD>
 +
    <TD>
 +
    157
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273043">BBa_K273043</a>
 +
    </TD>
 +
    <TD>
 +
    Intermediate
 +
    </TD>
 +
    <TD>
 +
    E24 - Ter
 +
    </TD>
 +
 
 +
    <TD>
 +
    187
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273044">BBa_K273044</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    Composite
 +
    </TD>
 +
    <TD>
 +
    pPetE - E1A1 - Ter
 +
    </TD>
 +
    <TD>
 +
    594
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273045">BBa_K273045</a>
 +
    </TD>
 +
    <TD>
 +
    Composite
 +
    </TD>
 +
    <TD>
 +
    pPetE - E1A2 - Ter
 +
    </TD>
 +
 
 +
    <TD>
 +
    604
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273046">BBa_K273046</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    Composite
 +
    </TD>
 +
    <TD>
 +
    pPetE - E1A3 - Ter
 +
    </TD>
 +
    <TD>
 +
    624
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273047">BBa_K273047</a>
 +
    </TD>
 +
    <TD>
 +
    Composite
 +
    </TD>
 +
    <TD>
 +
    pPetE - E1A4 - Ter
 +
    </TD>
 +
 
 +
    <TD>
 +
    604
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273048">BBa_K273048</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    Composite
 +
    </TD>
 +
    <TD>
 +
    pPetE - E1B1 - Ter
 +
    </TD>
 +
    <TD>
 +
    604
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273049">BBa_K273049</a>
 +
    </TD>
 +
    <TD>
 +
    Composite
 +
    </TD>
 +
    <TD>
 +
    pPetE - E1B2 - Ter
 +
    </TD>
 +
 
 +
    <TD>
 +
    604
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273050">BBa_K273050</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    Composite
 +
    </TD>
 +
    <TD>
 +
    pPetE - E1B3 - Ter
 +
    </TD>
 +
    <TD>
 +
    594
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273051">BBa_K273051</a>
 +
    </TD>
 +
    <TD>
 +
    Composite
 +
    </TD>
 +
    <TD>
 +
    pPetE - E1B4 - Ter
 +
    </TD>
 +
 
 +
    <TD>
 +
    611
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273052">BBa_K273052</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    Composite
 +
    </TD>
 +
    <TD>
 +
    pPetE - E21 - Ter
 +
    </TD>
 +
    <TD>
 +
    604
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273053">BBa_K273053</a>
 +
    </TD>
 +
    <TD>
 +
    Composite
 +
    </TD>
 +
    <TD>
 +
    pPetE - E22 - Ter
 +
    </TD>
 +
 
 +
    <TD>
 +
    604
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273054">BBa_K273054</a>
 +
    </TD>
 +
    <TD>
 +
 
 +
    Composite
 +
    </TD>
 +
    <TD>
 +
    pPetE - E23 - Ter
 +
    </TD>
 +
    <TD>
 +
    594
 +
    </TD>
 +
  </TR>
 +
  <TR>
 +
 
 +
    <TD>
 +
    <a class="UBBlink" href="http://partsregistry.org/Part:BBa_K273055">BBa_K273055</a>
 +
    </TD>
 +
    <TD>
 +
    Composite
 +
    </TD>
 +
    <TD>
 +
    pPetE - E24 - Ter
 +
    </TD>
 +
 
 +
    <TD>
 +
    624
 +
    </TD>
 +
  </TR>
 +
</TABLE>
 +
</body>
 +
</html>
 +
 
{{Uppsala-Sweden_Footer}}
{{Uppsala-Sweden_Footer}}

Latest revision as of 17:34, 19 October 2009




Contents


Parts

Here we list all the BioBricks we designed, constructed and used during the project.

Regulatory Elements

Promoter
BBa_K273019 Regulatory pPetE 429

Alcohol Production

Butanol Parts, Intermediates and Devices
BBa_K273006 Coding a-ketoisovalerate decarboxylase (kivd) codon optimized 1650
BBa_K273008 Intermediate RBS - a-ketoisovalerate decarboxylase (kivd) codon optimized 1668
BBa_K273005 Coding Alcohol Dehydrogenase 2 (Yadh2) 1050
BBa_K273007 Intermediate RBS - Alcohol Dehydrogenase 2 (Yadh2) 1068
BBa_K273009 Intermediate RBS - adh2(Y) - Terminator 1205
BBa_K273012 Intermediate RBS - kivd - RBS - adh2 - Ter 2881
BBa_K273011 Composite PLlac - RBS - kivd - RBS - adh2(Y) - Ter 2944
BBa_K273012 Composite pPetE - RBS - kivd - RBS - adh2(Y) - Ter 3318

Ethanol Parts and Devices
BBa_K273000 Coding Pyruvate decarboxylase (pdc) 1710
BBa_K273001 Coding Alcohol dehydrogenase (Zadh2) 1155
BBa_K273004 Intermediate RBS - Pyruvate decarboxylase (pdc) 1728
BBa_K273017 Intermediate RBS - Alcohol dehydrogenase (Zadh2) 1173
BBa_K273018 Intermediate RBS - adh2(Z) - Ter 1310
BBa_K273013 Intermediate RBS - pdc - RBS -adh2(Z) - Ter 3046
BBa_K273056 Composite PLlac - RBS - pdc - RBS - adh2(Z) - Ter 3109
BBa_K273057 Composite pPetE - RBS - pdc - RBS - adh2(Z) - Ter 3483

Ethanol - S. cerevisae
BBa_K273014 Intermediate RBS - pdc - RBS - adh2(Y) - Ter 2941
BBa_K273015 Composite PLlac - RBS - pdc - RBS - adh2(Y) - Ter 3004
BBa_K273016 Composite pPetE - RBS - pdc - RBS - adh2(Y) - Ter 3378

Pyruvate Level Increasing Approaches

Pir Approach
BBa_K273002 Coding Pirin like protein (pirA) 702
BBa_K273003 Coding Pirin like protein (pirB) 240

Antisense RNA Approach
BBa_K273020 RNA E1A1 20
BBa_K273021 RNA E1A2 30
BBa_K273022 RNA E1A3 50
BBa_K273023 RNA E1A4 30
BBa_K273024 RNA E1B1 30
BBa_K273025 RNA E1B2 30
BBa_K273026 RNA E1B3 20
BBa_K273027 RNA E1B4 37
BBa_K273028 RNA E21 30
BBa_K273029 RNA E22 30
BBa_K273030 RNA E23 20
BBa_K273031 RNA E24 50
BBa_K273032 Intermediate E1A1 - Ter 157
BBa_K273033 Intermediate E1A2 - Ter 167
BBa_K273034 Intermediate E1A3 - Ter 187
BBa_K273035 Intermediate E1A4 - Ter 167
BBa_K273036 Intermediate E1B1 - Ter 167
BBa_K273037 Intermediate E1B2 - Ter 167
BBa_K273038 Intermediate E1B3 - Ter 157
BBa_K273039 Intermediate E1B4 - Ter 174
BBa_K273040 Intermediate E21 - Ter 167
BBa_K273041 Intermediate E22 - Ter 167
BBa_K273042 Intermediate E23 - Ter 157
BBa_K273043 Intermediate E24 - Ter 187
BBa_K273044 Composite pPetE - E1A1 - Ter 594
BBa_K273045 Composite pPetE - E1A2 - Ter 604
BBa_K273046 Composite pPetE - E1A3 - Ter 624
BBa_K273047 Composite pPetE - E1A4 - Ter 604
BBa_K273048 Composite pPetE - E1B1 - Ter 604
BBa_K273049 Composite pPetE - E1B2 - Ter 604
BBa_K273050 Composite pPetE - E1B3 - Ter 594
BBa_K273051 Composite pPetE - E1B4 - Ter 611
BBa_K273052 Composite pPetE - E21 - Ter 604
BBa_K273053 Composite pPetE - E22 - Ter 604
BBa_K273054 Composite pPetE - E23 - Ter 594
BBa_K273055 Composite pPetE - E24 - Ter 624







Bioneer Biolegio Clontech Uppsala Genome Center