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Team:Uppsala-Sweden/pir
From 2009.igem.org
The Theory
The protein Pirin has in Serratia marcescens been shown to regulate the pyruvate levels by means of inhibition of the PDC [1]. While the Pirin superfamily is diverse and has different functions across different organisms it has been linked to growth inhibition in Synechocystis sp. PCC 6803 [2]. One could assume that this is done in the same way as in S. marcescens, by inhibiting the PDC and thus choking the Krebs cycle. In S. marcescens this leads to induction of fermentation pathways.
As a side effect we have found data on the ethanol resilience of Synechocysti, obtained through a test of induction of Pirin by ethanol stress [2], it is here shown that ethanol up to 5% in the growth media has a positive growth effect while it is detrimental to growth at 7.5%. Inhibited growth may be accepteble to us as we would typically be at optimum population density during ethanol production in either case. This proposes that we could tolerate concentrations higher then 7.5%.
The Constructs
and : The desired Construct for Testing Purposes
References
