Team:Utah State

From 2009.igem.org

(Difference between revisions)
Line 68: Line 68:
     <td>
     <td>
       <font size="6" face="Century Gothic, Arial, San Serif" color =#000033>
       <font size="6" face="Century Gothic, Arial, San Serif" color =#000033>
-
               <b><i>Welcome!</b></i>
+
               <b><i>BioBricks without Borders:</b></i></font>
-
      </font>
+
              <p><font face="Helvetica, Arial, San Serif" color =green>Investigating a multi-host BioBrick vector and secretion of cellular products</font></p><HR>
-
            <p> <font size="2" face="Helvetica, Arial, San Serif" color =#222222></br>You are looking at the 2009 Utah State University iGEM team wiki-page. </br></P>
+
<p> <font face="Helvetica, Arial, San Serif" color =#000000>The aim of the Utah State University iGEM project is to develop improved upstream and downstream processing strategies for manufacturing cellular products using the standardized BioBrick system. First, we altered the broad-host range vector pRL1383a to comply with BioBrick standards and enable use of BioBrick constructs in organisms like Pseudomonas putida, Rhodobacter sphaeroides, and Synechocystis PCC6803. This vector will facilitate exploitation of advantageous characteristics of these organisms, such as photosynthetic carbon assimilation.  Following expression, product recovery poses a difficult and expensive challenge. Downstream processing of cellular compounds, like polyhydroxyalkanoates (PHAs), commonly represents more than half of the total production expenseTo counter this problem, secretion-promoting BioBrick devices were constructed through genetic fusion of signal peptides with protein-coding regions.  To demonstrate this, the secretion of PHA granule-associated proteins and their affinity to PHA was investigated. Project success will facilitate expression and recovery of BioBrick-coded products in multiple organisms.</p>
-
            <p> <font size="2" face="Helvetica, Arial, San Serif" color =#222222>In the interest of creating a universal BioBrick vector, the goal of our project is to improve, modify, and test the broad-host vector pRL 1383a. After ensuring that the vector functions properly, we will test in the cyanobacterium <i>Synechocystis</i>, <i>Pseudomonas putida</i>, and <i>Escherichia coli</i>We will then develop expression systems in Synechocystis to produce various proteins. </font></p>
+
          </font>
-
      </font>
+
             <p>  <font size="4" face="Helvetica, Arial, San Serif" color =green>OUR SITE IS STILL UNDER CONSTRUCTION AND OUR INFORMATION IS BEING ADDED.  PLEASE COME BACK IN A FEW WEEKS TO SEE OUR PROJECT!</font></p>
             <p>  <font size="4" face="Helvetica, Arial, San Serif" color =green>OUR SITE IS STILL UNDER CONSTRUCTION AND OUR INFORMATION IS BEING ADDED.  PLEASE COME BACK IN A FEW WEEKS TO SEE OUR PROJECT!</font></p>
     </td>
     </td>

Revision as of 21:51, 9 October 2009

USU iGem Untitled Document


BioBricks without Borders:

Investigating a multi-host BioBrick vector and secretion of cellular products


The aim of the Utah State University iGEM project is to develop improved upstream and downstream processing strategies for manufacturing cellular products using the standardized BioBrick system. First, we altered the broad-host range vector pRL1383a to comply with BioBrick standards and enable use of BioBrick constructs in organisms like Pseudomonas putida, Rhodobacter sphaeroides, and Synechocystis PCC6803. This vector will facilitate exploitation of advantageous characteristics of these organisms, such as photosynthetic carbon assimilation. Following expression, product recovery poses a difficult and expensive challenge. Downstream processing of cellular compounds, like polyhydroxyalkanoates (PHAs), commonly represents more than half of the total production expense. To counter this problem, secretion-promoting BioBrick devices were constructed through genetic fusion of signal peptides with protein-coding regions. To demonstrate this, the secretion of PHA granule-associated proteins and their affinity to PHA was investigated. Project success will facilitate expression and recovery of BioBrick-coded products in multiple organisms.

OUR SITE IS STILL UNDER CONSTRUCTION AND OUR INFORMATION IS BEING ADDED. PLEASE COME BACK IN A FEW WEEKS TO SEE OUR PROJECT!


This is where more text can go