http://2009.igem.org/wiki/index.php?title=Team:Valencia/WetLab/YeastTeam/Experimental&feed=atom&action=historyTeam:Valencia/WetLab/YeastTeam/Experimental - Revision history2024-03-29T13:51:07ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:Valencia/WetLab/YeastTeam/Experimental&diff=159726&oldid=prevEmilio at 23:31, 21 October 20092009-10-21T23:31:53Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> height: <del class="diffchange diffchange-inline">8250px</del>;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> height: <ins class="diffchange diffchange-inline">8150px</ins>;</div></td></tr>
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</table>Emiliohttp://2009.igem.org/wiki/index.php?title=Team:Valencia/WetLab/YeastTeam/Experimental&diff=159717&oldid=prevEmilio at 23:31, 21 October 20092009-10-21T23:31:38Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> height: <del class="diffchange diffchange-inline">7750px</del>;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> height: <ins class="diffchange diffchange-inline">8250px</ins>;</div></td></tr>
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</table>Emiliohttp://2009.igem.org/wiki/index.php?title=Team:Valencia/WetLab/YeastTeam/Experimental&diff=159656&oldid=prevArnau at 23:29, 21 October 20092009-10-21T23:29:54Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Valencia_luminometer.JPG|left|300 px]]However, in order to make measurements with the continuous luminometer, we needed to prevent light entering inside the machine, as in all the other cases, just at the moment of the input. In that particular device, we had to be extremely accurate: our continuous luminometer is very sensitive and natural light disables it when the sensor gets too dilated. When that happens, the luminometer needs about a week to return to its proper state.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Valencia_luminometer.JPG|left|300 px]]However, in order to make measurements with the continuous luminometer, we needed to prevent light entering inside the machine, as in all the other cases, just at the moment of the input. In that particular device, we had to be extremely accurate: our continuous luminometer is very sensitive and natural light disables it when the sensor gets too dilated. When that happens, the luminometer needs about a week to return to its proper state.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We built a structure similar to a protective helmet made of termaflex, with a little hole where the cables cross it, and put that construction on the top of the measurement point, where our sample must be placed. Any entrance of light was prevented and we measured luminiscence without problems</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We built a structure similar to a protective helmet made of termaflex, with a little hole where the cables cross it, and put that construction on the top of the measurement point, where our sample must be placed. Any entrance of light was prevented and we measured luminiscence without problems</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br><br><br><br><br><br><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Protocols==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Protocols==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Measurement of citoplasmic Ca<sup>2+</sup> increase in <i>S. cerevisiae</i>===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Measurement of citoplasmic Ca<sup>2+</sup> increase in <i>S. cerevisiae</i>===</div></td></tr>
</table>Arnauhttp://2009.igem.org/wiki/index.php?title=Team:Valencia/WetLab/YeastTeam/Experimental&diff=159226&oldid=prevCristina VS: /* LUMINOMETER */2009-10-21T23:16:45Z<p><span class="autocomment">LUMINOMETER</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Each luminometer has its own protocol.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Each luminometer has its own protocol.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>First, we work with a discontinous luminometer. It measures every 30 seconds<del class="diffchange diffchange-inline">, what means that we have no information about what happens during this period of time</del>. We make a lot of measurements, trying to optimize the electrical input with the light generation. It's connected to a computer, where we see the value of luminescence. To measure luminescence, luminometer have a Elisa plate, where we put our yeasts. After, we introduce the Elisa plate into luminometer and click Start on computer. After 15 seconds we have the measure of luminosity.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>First, we work with a discontinous luminometer. It measures every 30 seconds. We make a lot of measurements, trying to optimize the electrical input with the light generation. It's connected to a computer, where we see the value of luminescence. To measure luminescence, luminometer have a Elisa plate, where we put our yeasts. After, we introduce the Elisa plate into luminometer and click Start on computer. After 15 seconds we have the measure of luminosity.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After, in the same department, we used a continuous luminometer. This is better because measures instantly, every second and the results obtained are more reliable. With this two luminometer we make the caracterization of ''Aequorin'' and obtained the results that demonstrate that we were right. WE CAN CREATE A CELL-BASED BIOSCREEN. Continuous luminometer have a cuvette where we put the cells. The cuvette must be very closed. We devise a system to applicate the electrical stimulus when the cuvette is closed. This luminometer also have a computer, but it was very old!</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After, in the same department, we used a continuous luminometer. This is better because measures instantly, every second and the results obtained are more reliable. With this two luminometer we make the caracterization of ''Aequorin'' and obtained the results that demonstrate that we were right. WE CAN CREATE A CELL-BASED BIOSCREEN. Continuous luminometer have a cuvette where we put the cells. The cuvette must be very closed. We devise a system to applicate the electrical stimulus when the cuvette is closed. This luminometer also have a computer, but it was very old!</div></td></tr>
</table>Cristina VShttp://2009.igem.org/wiki/index.php?title=Team:Valencia/WetLab/YeastTeam/Experimental&diff=158781&oldid=prevCristina VS: /* LUMINOMETER */2009-10-21T23:02:38Z<p><span class="autocomment">LUMINOMETER</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>At last, we found a luminometer at Instituto de química molecular aplicada at UPV. Luminometers are more sensible and have more precision than espectrophotometer and espectrofluorimeter. Luminometer is ideal to work with aequorin, but was difficult to us to find one (we were looking for one and found two ^^).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>At last, we found a luminometer at Instituto de química molecular aplicada at UPV. Luminometers are more sensible and have more precision than espectrophotometer and espectrofluorimeter. Luminometer is ideal to work with aequorin, but was difficult to us to find one (we were looking for one and found two ^^).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>There are two types of luminometers: continuous and discontinuous. The discontinuous make punctual measures, in our case every 30 seconds. The continuous measures continuously, every second. Is important to note that we use two different luminometer, provided by different manufacturers. For this reasons we can't compare directly the results obtained with one luminometer with the results of the other. According this, <del class="diffchange diffchange-inline">when </del>we only compare results in the same graph if they were obtained with the same luminometer. However, an increase (or not) in the luminosity, means the same at two luminometers and the experiments are complementary and reaffirms our conclusions. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>There are two types of luminometers: continuous and discontinuous. The discontinuous make punctual measures, in our case every 30 seconds. The continuous measures continuously, every second. Is important to note that we use two different luminometer, provided by different manufacturers. For this reasons we can't compare directly the results obtained with one luminometer with the results of the other. According this, we only compare results in the same graph if they were obtained with the same luminometer. However, an increase (or not) in the luminosity, means the same at two luminometers and the experiments are complementary and reaffirms our conclusions. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Each luminometer has its own protocol.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Each luminometer has its own protocol.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>First, we work with a discontinous luminometer. It measures every 30 seconds. We make a lot of measurements, trying to optimize the electrical input with the light generation. It's connected to a computer, where we see the value of luminescence. To measure luminescence, luminometer have a Elisa plate, where we put our yeasts. After, we introduce the Elisa plate into luminometer and click Start on computer. After 15 seconds we have the measure of luminosity.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>First, we work with a discontinous luminometer. It measures every 30 seconds<ins class="diffchange diffchange-inline">, what means that we have no information about what happens during this period of time</ins>. We make a lot of measurements, trying to optimize the electrical input with the light generation. It's connected to a computer, where we see the value of luminescence. To measure luminescence, luminometer have a Elisa plate, where we put our yeasts. After, we introduce the Elisa plate into luminometer and click Start on computer. After 15 seconds we have the measure of luminosity.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After, in the same department, we used a continuous luminometer. This is better because measures instantly, every second and the results obtained are more reliable. With this two luminometer we make the caracterization of ''Aequorin'' and obtained the results that demonstrate that we were right. WE CAN CREATE A CELL-BASED BIOSCREEN. Continuous luminometer have a cuvette where we put the cells. The cuvette must be very closed. We devise a system to applicate the electrical stimulus when the cuvette is closed. This luminometer also have a computer, but it was very old!</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After, in the same department, we used a continuous luminometer. This is better because measures instantly, every second and the results obtained are more reliable. With this two luminometer we make the caracterization of ''Aequorin'' and obtained the results that demonstrate that we were right. WE CAN CREATE A CELL-BASED BIOSCREEN. Continuous luminometer have a cuvette where we put the cells. The cuvette must be very closed. We devise a system to applicate the electrical stimulus when the cuvette is closed. This luminometer also have a computer, but it was very old!</div></td></tr>
</table>Cristina VShttp://2009.igem.org/wiki/index.php?title=Team:Valencia/WetLab/YeastTeam/Experimental&diff=158389&oldid=prevArnau at 22:52, 21 October 20092009-10-21T22:52:28Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>With the discontinuous luminometer, we couldn't measure live, so we didn't desinged any kind of natural-light-protector for the sample while we were measuring.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>With the discontinuous luminometer, we couldn't measure live, so we didn't desinged any kind of natural-light-protector for the sample while we were measuring.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Valencia_luminometer.JPG|300 px]]However, in order to make measurements with the continuous luminometer, we needed to prevent light entering inside the machine, as in all the other cases, just at the moment of the input. In that particular device, we had to be extremely accurate: our continuous luminometer is very sensitive and natural light disables it when the sensor gets too dilated. When that happens, the luminometer needs about a week to return to its proper state.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Valencia_luminometer.JPG<ins class="diffchange diffchange-inline">|left</ins>|300 px]]However, in order to make measurements with the continuous luminometer, we needed to prevent light entering inside the machine, as in all the other cases, just at the moment of the input. In that particular device, we had to be extremely accurate: our continuous luminometer is very sensitive and natural light disables it when the sensor gets too dilated. When that happens, the luminometer needs about a week to return to its proper state.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We built a structure similar to a protective helmet made of termaflex, with a little hole where the cables cross it, and put that construction on the top of the measurement point, where our sample must be placed. Any entrance of light was prevented and we measured luminiscence without problems</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We built a structure similar to a protective helmet made of termaflex, with a little hole where the cables cross it, and put that construction on the top of the measurement point, where our sample must be placed. Any entrance of light was prevented and we measured luminiscence without problems</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Arnauhttp://2009.igem.org/wiki/index.php?title=Team:Valencia/WetLab/YeastTeam/Experimental&diff=158023&oldid=prevArnau: /* LUMINOMETER */2009-10-21T22:43:36Z<p><span class="autocomment">LUMINOMETER</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>With the discontinuous luminometer, we couldn't measure live, so we didn't desinged any kind of natural-light-protector for the sample while we were measuring.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>With the discontinuous luminometer, we couldn't measure live, so we didn't desinged any kind of natural-light-protector for the sample while we were measuring.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">But </del>in order to make measurements with the continuous luminometer, we needed to prevent light entering inside the machine, as in all the other cases, just at the moment of the input. In that particular device, we had to be extremely accurate: our continuous luminometer is very sensitive and natural light disables it when the sensor gets too dilated. When that happens, the luminometer needs about a week to return to its proper state.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[Image:Valencia_luminometer.JPG|300 px]]However, </ins>in order to make measurements with the continuous luminometer, we needed to prevent light entering inside the machine, as in all the other cases, just at the moment of the input. In that particular device, we had to be extremely accurate: our continuous luminometer is very sensitive and natural light disables it when the sensor gets too dilated. When that happens, the luminometer needs about a week to return to its proper state.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We built a structure similar to a protective helmet made of termaflex, with a little hole where the cables cross it, and put that construction on the top of the measurement point, where our sample must be placed. Any entrance of light was prevented and we measured luminiscence without problems</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We built a structure similar to a protective helmet made of termaflex, with a little hole where the cables cross it, and put that construction on the top of the measurement point, where our sample must be placed. Any entrance of light was prevented and we measured luminiscence without problems</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[Image:Valencia_luminometer.JPG|300 px]]</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Protocols==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Protocols==</div></td></tr>
</table>Arnauhttp://2009.igem.org/wiki/index.php?title=Team:Valencia/WetLab/YeastTeam/Experimental&diff=157918&oldid=prevArnau: /* LUMINOMETER */2009-10-21T22:40:25Z<p><span class="autocomment">LUMINOMETER</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After, in the same department, we used a continuous luminometer. This is better because measures instantly, every second and the results obtained are more reliable. With this two luminometer we make the caracterization of ''Aequorin'' and obtained the results that demonstrate that we were right. WE CAN CREATE A CELL-BASED BIOSCREEN. Continuous luminometer have a cuvette where we put the cells. The cuvette must be very closed. We devise a system to applicate the electrical stimulus when the cuvette is closed. This luminometer also have a computer, but it was very old!</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After, in the same department, we used a continuous luminometer. This is better because measures instantly, every second and the results obtained are more reliable. With this two luminometer we make the caracterization of ''Aequorin'' and obtained the results that demonstrate that we were right. WE CAN CREATE A CELL-BASED BIOSCREEN. Continuous luminometer have a cuvette where we put the cells. The cuvette must be very closed. We devise a system to applicate the electrical stimulus when the cuvette is closed. This luminometer also have a computer, but it was very old!</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:HiTech! val.jpg|center|315 px|]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:HiTech! val.jpg|center|315 px<ins class="diffchange diffchange-inline">]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">With the discontinuous luminometer, we couldn't measure live, so we didn't desinged any kind of natural-light-protector for the sample while we were measuring.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">But in order to make measurements with the continuous luminometer, we needed to prevent light entering inside the machine, as in all the other cases, just at the moment of the input. In that particular device, we had to be extremely accurate: our continuous luminometer is very sensitive and natural light disables it when the sensor gets too dilated. When that happens, the luminometer needs about a week to return to its proper state.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">We built a structure similar to a protective helmet made of termaflex, with a little hole where the cables cross it, and put that construction on the top of the measurement point, where our sample must be placed. Any entrance of light was prevented and we measured luminiscence without problems</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[Image:Valencia_luminometer.JPG</ins>|<ins class="diffchange diffchange-inline">300 px</ins>]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Protocols==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Protocols==</div></td></tr>
</table>Arnauhttp://2009.igem.org/wiki/index.php?title=Team:Valencia/WetLab/YeastTeam/Experimental&diff=149549&oldid=prevCristina VS: /* Preparing vectors */2009-10-21T17:36:11Z<p><span class="autocomment">Preparing vectors</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Preparing vectors===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Preparing vectors===</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Competent cells were transformed with pSB1A3 with the J04450 insert (present in the kit plate 1, hole 1K from the 2009 plasmid backbone distribution kit). We used the transformation protocol of <del class="diffchange diffchange-inline">XL1</del>-Gold Ultracompetent Cells of<del class="diffchange diffchange-inline">....</del>. We selected transformed cells in a LB + ampicillin medium plaques. <br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Competent cells were transformed with pSB1A3 with the J04450 insert (present in the kit plate 1, hole 1K from the 2009 plasmid backbone distribution kit). We used the transformation protocol of <ins class="diffchange diffchange-inline">XL10</ins>-Gold Ultracompetent Cells of <ins class="diffchange diffchange-inline">Stratagene</ins>. We selected transformed cells in a LB + ampicillin medium plaques. <br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The following day, we selected red colonies, those that had the plasmid, and plasmids were extracted with the High pure miniprep plasmid isolation kit (ROCHE) <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The following day, we selected red colonies, those that had the plasmid, and plasmids were extracted with the High pure miniprep plasmid isolation kit (ROCHE) <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Plasmid were digested with EcoRI and XbaI, in the same way we digested PCR result.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Plasmid were digested with EcoRI and XbaI, in the same way we digested PCR result.<br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Ligating BioBricks into plasmids===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Ligating BioBricks into plasmids===</div></td></tr>
</table>Cristina VShttp://2009.igem.org/wiki/index.php?title=Team:Valencia/WetLab/YeastTeam/Experimental&diff=146875&oldid=prevLalo at 15:36, 21 October 20092009-10-21T15:36:22Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">__NOTOC__</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{| </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{| </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>!Methods</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>!Methods</div></td></tr>
</table>Lalo