http://2009.igem.org/wiki/index.php?title=Team:Valencia/proves8&feed=atom&action=historyTeam:Valencia/proves8 - Revision history2024-03-28T14:17:00ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:Valencia/proves8&diff=94041&oldid=prevGuimar3: New page: __NOTOC__ {{Template:Valencia09iGEM6}} <div align="justify" style="position:relative; top:-5px; left:70px; width:600px"> == '''WetLab Overview''' == <span style="color:black; align:justi...2009-10-12T16:31:07Z<p>New page: __NOTOC__ {{Template:Valencia09iGEM6}} <div align="justify" style="position:relative; top:-5px; left:70px; width:600px"> == '''WetLab Overview''' == <span style="color:black; align:justi...</p>
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== '''WetLab Overview''' ==<br />
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At WetLab we are working with '''yeasts''' in order to create each and everyone of the cell-pixels of our bioscreen. Since we want to make yeasts shine, we are using an aequorin-transformed yeast strains.<br />
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'''Aequorin''' is a luminiscent protein that originally was isolated from luminescent jellyfish ''Aequorea''. It can also find in other species of ''Aequorea'' and in many other marine organisms. For more information about de discovery of aequorin, read [https://2009.igem.org/Team:Valencia/A_short_story '''A short story of Aequorin'''], written by Osamu Shimomura. This is an interesting and entertaining text. You should read it. No doubt!<br />
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To make light, Aequorin uses '''coelenterazine''' as its cofactor. Aequorin also needs the binding of Ca<SUP>2+</SUP> to produce light. You can see the complete reaction in the next picture:<br />
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[[Image:Aquorin reaction.gif|500px]]<br />
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<b>[https://static.igem.org/mediawiki/2009/3/35/Aequorin.GIF/ Aequorin]</b> + <b>[https://static.igem.org/mediawiki/2009/6/60/Coelenterazine.png/ Coelenterazine]</b> + Ca<SUP>2+</SUP> --> [[Image:Aquorin in action.jpg|350px]]<br />
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We will use a '''chemical input''' like KOH (alkali shock) to open '''Ca<SUP>2+</SUP> channels''' in the yeasts' membrane (Viladevall L, et al. J Biol Chem. (2004) 279 43614–43624), then Ca<SUP>2+</SUP> will enter into the cells and we will get '''light''' as an '''output'''.<br />
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If we succed in this, we will try to reproduce the same response with an '''electrical stimulus''', using some hardware built by ourselves. We will use an electrical input to open the Ca<SUP>2+</SUP> channels. We hope that we see light by this metod.<br />
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