Team:VictoriaBC/Design

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Revision as of 07:58, 20 October 2009

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OVERVIEW

DESIGN

TESTING

RESULTS

CONCLUSIONS

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LAB NOTEBOOK

LAB PROTOCOLS

PARTS

DESIGN
The final project consisted of a simplified biothermometer design. Using the 32°C ribothermometer linked to either RFP or GFP would entail a system that responded visually to differences in temperature. There were two levels for this: K235034 and K235035 used a lactose promoter and repressor to regulate RFP and GFP respectively; and K235036 and K235037 were directly coupled to RFP or GFP respectively.
K235034

K235034 (Chl) const. promoter+32oC temp. sensing RBS	K235033(Kan) const. promoter+32oC temp. sensing RBS	J04450(Kan) Lac controlled RFP	P1010-Chl
K235035 (Chl) const. promoter+32oC temp. sensing RBS+ lactose promoter+RBS+GFP+stop	K235033(Kan) const. promoter+32oC temp. sensing RBS	K235032(Kan) lactose promoter+RBS+GFP+stop	P1010-Chl
K235036 (Kan) const. promoter+32oC temp. sensing RBS+RFP+stop	K235030(Chl) const. promoter+32oC temp. sensing RBS	K235005(Chl) RFP+stop	P1010-Kan
K235037 (Chl) const. promoter+32oC temp. sensing RBS+GFP+stop	K235030(Kan) const. promoter+32oC temp. sensing RBS	J04630(Amp/Kan) GFP+stop	P1010-Chl
K235038 (Chl) const. promoter+RBS	J23102(Amp) const. promoter	B0034 (Amp) RBS	P1010-Chl
K235038 (Kan) const. promoter+RBS	J23102(Amp) const. promoter	B0034 (Amp) RBS	P1010-Kan
K235039 (Kan) const. promoter+RBS+LacI repressor+LVA+stop	K235038 (Chl) const. promoter+RBS	K235031 (Chl) LacI repressor+LVA+stop	P1010-Kan
K235040 (Chl) const. promoter+RBS+Lac controlled RFP	K235039 (Kan) const. promoter+RBS	J04450(Kan) Lac controlled RFP	P1010-Chl
K235041 (Chl) const. promoter+RBS+lactose promoter+RBS+GFP+stop	K235039 (Kan) const. promoter+RBS	K235032(Kan) lactose promoter+RBS+GFP+stop	P1010-Chl
K235042 (Kan) const. promoter+RBS+RFP+stop	K235038 (Chl) const. promoter+RBS	K235005(Chl) RFP+stop	P1010-Kan
K235043 (Chl) const. promoter+RBS+GFP+stop	K235038 (Kan) const. promoter+RBS	J04630(Amp/Kan) GFP+stop	P1010-Chl

@@ Line 38: / Line 38: @@
 <table cellspacing=0>
   <tr>
+  <td valign="top">
+  <table>
+  <tr>
    <td width="210" bgcolor="#990000" valign="top">
    <center>
    <a href="https://2009.igem.org/Team:VictoriaBC">HOME</a>
    </td>
-<td width="10" rowspan=10 bgcolor="#163216">
-<td width="750" rowspan=30 bgcolor="#e4e4fe" valign="top">
-DESIGN<br>
-The final project consisted of a simplified biothermometer design.  Using the 32&deg;C ribothermometer linked to either RFP or GFP would entail a system that responded visually to differences in temperature.  There were two levels for this:  K235034 and K235035 used a lactose promoter and repressor to regulate RFP and GFP respectively; and K235036 and K235037 were directly coupled to RFP or GFP respectively.<br>
-K235034<br>
-<table>
-<tr>
-            <td>K235034 (Chl) const. promoter+32oC temp. sensing RBS</td>
-            <td>K235033(Kan) const. promoter+32oC temp. sensing RBS</td>
-            <td><a href="http://partsregistry.org/Part:BBa_J04450">J04450</a>(Kan) Lac controlled RFP</td>
-            <td>P1010-Chl</td>
-        </tr>
-        <tr>
-            <td>K235035 (Chl) const. promoter+32oC temp. sensing RBS+ lactose promoter+RBS+GFP+stop</td>
-            <td>K235033(Kan) const. promoter+32oC temp. sensing RBS</td>
-            <td>K235032(Kan) lactose promoter+RBS+GFP+stop</td>
-            <td>P1010-Chl</td>
-        </tr>
-        <tr>
-            <td>K235036 (Kan) const. promoter+32oC temp. sensing RBS+RFP+stop</td>
-            <td>K235030(Chl) const. promoter+32oC temp. sensing RBS</td>
-            <td>K235005(Chl) RFP+stop</td>
-            <td>P1010-Kan</td>
-        </tr>
-        <tr>
-            <td>K235037 (Chl) const. promoter+32oC temp. sensing RBS+GFP+stop</td>
-            <td>K235030(Kan) const. promoter+32oC temp. sensing RBS</td>
-            <td><a href="http://partsregistry.org/Part:BBa_J04630">J04630</a>(Amp/Kan) GFP+stop</td>
-            <td>P1010-Chl</td>
-        </tr>
-        <tr>
-            <td>K235038 (Chl) const. promoter+RBS</td>
-            <td><a href="http://partsregistry.org/Part:BBa_J23102">J23102</a>(Amp) const. promoter</td>
-            <td><a href="http://partsregistry.org/Part:BBa_B0034">B0034</a> (Amp) RBS</td>
-            <td>P1010-Chl</td>
-        </tr>
-        <tr>
-            <td>K235038 (Kan) const. promoter+RBS</td>
-            <td><a href="http://partsregistry.org/Part:BBa_J23102">J23102</a>(Amp) const. promoter</td>
-            <td><a href="http://partsregistry.org/Part:BBa_B0034">B0034</a> (Amp) RBS</td>
-            <td>P1010-Kan</td>
-        </tr>
-        <tr>
-            <td>K235039 (Kan) const. promoter+RBS+LacI repressor+LVA+stop</td>
-            <td>K235038 (Chl) const. promoter+RBS</td>
-            <td>K235031 (Chl) LacI repressor+LVA+stop</td>
-            <td>P1010-Kan</td>
-        </tr>
-        <tr>
-            <td>K235040 (Chl) const. promoter+RBS+Lac controlled RFP</td>
-            <td>K235039 (Kan) const. promoter+RBS</td>
-            <td><a href="http://partsregistry.org/Part:BBa_J04450">J04450</a>(Kan) Lac controlled RFP</td>
-            <td>P1010-Chl</td>
-        </tr>
-        <tr>
-            <td>K235041 (Chl) const. promoter+RBS+lactose promoter+RBS+GFP+stop</td>
-            <td>K235039 (Kan) const. promoter+RBS</td>
-            <td>K235032(Kan) lactose promoter+RBS+GFP+stop</td>
-            <td>P1010-Chl</td>
-        </tr>
-        <tr>
-            <td>K235042 (Kan) const. promoter+RBS+RFP+stop</td>
-            <td>K235038 (Chl) const. promoter+RBS</td>
-            <td>K235005(Chl) RFP+stop</td>
-            <td>P1010-Kan</td>
-        </tr>
-        <tr>
-            <td>K235043 (Chl) const. promoter+RBS+GFP+stop</td>
-            <td>K235038 (Kan) const. promoter+RBS</td>
-            <td><a href="http://partsregistry.org/Part:BBa_J04630">J04630</a>(Amp/Kan) GFP+stop</td>
-            <td>P1010-Chl</td>
-        </tr>
-</table>
   <tr>
    <td width="210" height="10" bgcolor="#163216">
@@ Line 155: / Line 82: @@
    <td width="210" bgcolor="#996600" valign="top">
    <center>
-<a href="https://2009.igem.org/Team:VictoriaBC/Testing">TESTING</a>
+<a href="https://2009.igem.org/Team:VictoriaBC/Tetsting">TESTING</a>
    </td>
   </tr>
@@ Line 222: / Line 149: @@
    </td>
   </tr>
+</table>
+</td>
+<td width="10" rowspan=10 bgcolor="#163216">
+<td width="750" rowspan=30 bgcolor="#e4e4fe" valign="top">
+DESIGN<br>
+The final project consisted of a simplified biothermometer design.  Using the 32&deg;C ribothermometer linked to either RFP or GFP would entail a system that responded visually to differences in temperature.  There were two levels for this:  K235034 and K235035 used a lactose promoter and repressor to regulate RFP and GFP respectively; and K235036 and K235037 were directly coupled to RFP or GFP respectively.<br>
+K235034<br>
+<table>
+<tr>
+            <td>K235034 (Chl) const. promoter+32oC temp. sensing RBS</td>
+            <td>K235033(Kan) const. promoter+32oC temp. sensing RBS</td>
+            <td><a href="http://partsregistry.org/Part:BBa_J04450">J04450</a>(Kan) Lac controlled RFP</td>
+            <td>P1010-Chl</td>
+        </tr>
+        <tr>
+            <td>K235035 (Chl) const. promoter+32oC temp. sensing RBS+ lactose promoter+RBS+GFP+stop</td>
+            <td>K235033(Kan) const. promoter+32oC temp. sensing RBS</td>
+            <td>K235032(Kan) lactose promoter+RBS+GFP+stop</td>
+            <td>P1010-Chl</td>
+        </tr>
+        <tr>
+            <td>K235036 (Kan) const. promoter+32oC temp. sensing RBS+RFP+stop</td>
+            <td>K235030(Chl) const. promoter+32oC temp. sensing RBS</td>
+            <td>K235005(Chl) RFP+stop</td>
+            <td>P1010-Kan</td>
+        </tr>
+        <tr>
+            <td>K235037 (Chl) const. promoter+32oC temp. sensing RBS+GFP+stop</td>
+            <td>K235030(Kan) const. promoter+32oC temp. sensing RBS</td>
+            <td><a href="http://partsregistry.org/Part:BBa_J04630">J04630</a>(Amp/Kan) GFP+stop</td>
+            <td>P1010-Chl</td>
+        </tr>
+        <tr>
+            <td>K235038 (Chl) const. promoter+RBS</td>
+            <td><a href="http://partsregistry.org/Part:BBa_J23102">J23102</a>(Amp) const. promoter</td>
+            <td><a href="http://partsregistry.org/Part:BBa_B0034">B0034</a> (Amp) RBS</td>
+            <td>P1010-Chl</td>
+        </tr>
+        <tr>
+            <td>K235038 (Kan) const. promoter+RBS</td>
+            <td><a href="http://partsregistry.org/Part:BBa_J23102">J23102</a>(Amp) const. promoter</td>
+            <td><a href="http://partsregistry.org/Part:BBa_B0034">B0034</a> (Amp) RBS</td>
+            <td>P1010-Kan</td>
+        </tr>
+        <tr>
+            <td>K235039 (Kan) const. promoter+RBS+LacI repressor+LVA+stop</td>
+            <td>K235038 (Chl) const. promoter+RBS</td>
+            <td>K235031 (Chl) LacI repressor+LVA+stop</td>
+            <td>P1010-Kan</td>
+        </tr>
+        <tr>
+            <td>K235040 (Chl) const. promoter+RBS+Lac controlled RFP</td>
+            <td>K235039 (Kan) const. promoter+RBS</td>
+            <td><a href="http://partsregistry.org/Part:BBa_J04450">J04450</a>(Kan) Lac controlled RFP</td>
+            <td>P1010-Chl</td>
+        </tr>
+        <tr>
+            <td>K235041 (Chl) const. promoter+RBS+lactose promoter+RBS+GFP+stop</td>
+            <td>K235039 (Kan) const. promoter+RBS</td>
+            <td>K235032(Kan) lactose promoter+RBS+GFP+stop</td>
+            <td>P1010-Chl</td>
+        </tr>
+        <tr>
+            <td>K235042 (Kan) const. promoter+RBS+RFP+stop</td>
+            <td>K235038 (Chl) const. promoter+RBS</td>
+            <td>K235005(Chl) RFP+stop</td>
+            <td>P1010-Kan</td>
+        </tr>
+        <tr>
+            <td>K235043 (Chl) const. promoter+RBS+GFP+stop</td>
+            <td>K235038 (Kan) const. promoter+RBS</td>
+            <td><a href="http://partsregistry.org/Part:BBa_J04630">J04630</a>(Amp/Kan) GFP+stop</td>
+            <td>P1010-Chl</td>
+        </tr>
+</table>
+</td>
+</tr>